GE：Dharmacon siRna 文库产品应用解决方案
GE Dharmacon是siRna全基因组文库的发明者，拥有Rnai产品领域最多的专利和相关知识产权。Dharmacon pre-defined siRna文库是目前应用最广泛、客户最值得信赖的siRna文库。同时，Dharmacon牵头成立的Rnai全球协议组织（RGI）的60多个知名科研院所成员使用Dharmacon siRna文库取得了良好的效果。siRna文库筛选加速了基因功能的研究，加深了疾病发生机理的研究，并获得了更多新的药物作用机理和潜在药物靶点，已经成为获得高质量研究数据和加速研究进程的必备工具。
Protein synthesis: mRna surveillance by the ribosome
Rachel Green (Johns Hopkins U., HHMI) 2: Protein synthesis: mRna surveillance by the ribosome
Talk Overview:
In her first talk, Green provides a detailed look at protein synthesis, or translation. Translation is the process by which nucleotides, the “language” of Dna and Rna, are translated into amino acids, the “language” of proteins. Green begins by describing the components needed for translation; mRna, tRna, ribosomes, and the initiation, elongation, and termination factors. She then explains the roles of these players in ensuring accuracy during the initiation, elongation, termination and recycling steps of the translation process. By comparing translation in bacteria and eukaryotes, Green explains that it is possible to determine which components and steps are highly conserved and predate the divergence of different kingdoms on the tree of life, and which are more recent adaptations.
Green’s second talk focuses on work from her lab investigating how ribosomes detect defective mRnas and trigger events leading to the degradation of the bad Rna and the incompletely translated protein product and to the recycling of the ribosome components. Working in yeast and using a number of biochemical and genetic techniques, Green’s lab showed that the protein Dom34 is critical for facilitating ribosome release from the short mRnas that result from mRna cleavage. Experiments showed that Dom34-mediated rescue of ribosomes from short mRnas is an essential process for cell survival in higher eukaryotes.
Speaker Biography:
Rachel Green received her BS in chemistry from the University of Michigan. She then moved to Harvard to pursue her PhD in the lab of Jack Szostak where she worked on designing catalytic Rna molecules and investigating their implications for the evolution of life. As a post-doctoral fellow at the University of California, Santa Cruz, Green began to study how the ribosome translates mRna to protein with such accuracy.
Currently, Green is a Professor of Molecular Biology and Genetics at the Johns Hopkins School of Medicine and an Investigator of the Howard Hughes Medical Institute. Research in her lab continues to focus on the ribosome and factors involved in the fidelity of eukaryotic and prokaryotic translation.
Green is the recipient of a Johns Hopkins University School of Medicine Graduate Teaching Award as well as the recipient for numerous awards for her research. She was elected to the national Academy of Sciences in 2012.
Dna聚合酶链式反应
What are Dna polymerases? Dna polymerases are enzymes responsible for assembling nucleotides to create new Dna molecules. These Dna polymerases are indispensable to cell divisions as they duplicate the genetic information that would be passed to the next generation of daughter cells. Throughout the years, scientists have utilized different variants of Dna polymerases for different applications.
从实验到分析——深度解析基于二代测序的LncRna研究及应用
非编码Rna起初被认为是基因组转录的“垃圾”，而近年来，多个高水平的杂志以封面形式报道长链非编码Rna（long non-coding Rna，LncRna）的重要性，由此LncRnas引起了人们广泛的关注，已成为各研究领域的一颗新星。
本次讲座主要将从以下几个方面展开：
①LncRna的方案设计及研究思路
②LncRna的实验及分析流程
③LncRna的案例解析
④LncRna+mRna+miRna联合分析方案解析
⑤一类特殊的ncRna——circular Rna
支原体检测：赫斯特Dna染色
This AbVideo tells you how to detect Mycoplasma in vero cell samples through the “Hoechst Dna staining method”, since one of the most important cell culture procedures is to use microtest to identify whether mycoplasmal contaminant is present or not. More videos at Abnova http://www.abnova.com
mircoRna是什么
戴维·巴特尔通过分别讲述决定一个microRna是什么、miRna在动物、C.elegans从线虫小Rna测序、注释的标准、小鼠miRna、实验性检验、试图在未编序的miRna的表达、在人类miRna的表达、试图在秀丽隐杆线虫miRna表达、miRna基因的预测、识别决定因素的方法、pri-miRna残留富集活性变体、中核集团其他一些miRna的重要主题、人体pri-miRna特征、试图在秀丽隐杆线虫miRna表达、增加一个人体pri-miRna特点蜗杆pri-miRna等阐明microRna是什么。
Snapshots of Metalloproteins in Action
1、金属蛋白定义 2、某些惊人的反应：固碳与氧的演化、无氧碳固定术、固氮（作用）、初级代谢的分子转变、核酸代谢的分子变换、建筑分子支架 、剪裁分子支架。 3、金属反应性的生物危害性 4、金属蛋白的应用研究 5、研究金属蛋白的技术挑战和益处 6、以快照的金属蛋白
将Rnascope技术应用于药物安全性评价
该视频由美国Advanced Cell Diagnostics公司的应用科学家讲解如何将Rnascope®技术应用于药物安全性评价。临床前药物安全性评价和毒性研究需要检测组织中的生物标志物。通过使用基于Leica BOND RX的全自动Rnascope® 2.5 LS检测试剂盒，对来自大鼠、猴、犬的FFPE样本进行Rna原位杂交检测。Rnascope® 2.5 LS检测在每种物种的25中不用组织中均展现了稳健地检测，具有高信噪比，并能够良好地维持组织形态。在这些检测的基础上，我们为各种类型组织的样本预处理条件以及阳性对照探针的选择提供建议。另外，对于特定的Rna标志物，如CD31、CD68、细胞增殖表达Ki-67和细胞周期标志Cyclin E1以及凋亡相关分子Puma、Fas、DR5均进行了有效检测。这项研究表明，Rnascope®2.5 LS检测是临床前安全性评价和动物研究中生物标志物分析的一个有力平台。 详细信息请访问ACD官网www.acdbio.com。更多中文资料请关注中国官方微信号（ACD_China）咨询。
将Rnascope技术应用于免疫治疗研究
该视频由美国Advanced Cell Diagnostics公司的应用科学家讲解如何将Rnascope®技术应用于免疫治疗研究。近年来，随着免疫检查点抑制剂和其他治疗方法的进行，肿瘤免疫治疗领域获得广泛关注。为了更好地对患者进行分层以及更好地研究肿瘤的微环境对肿瘤免疫的影响，需要对肿瘤免疫周期的生物学事件和生物标记物有更深入的了解，在单细胞水平对肿瘤及肿瘤微环境下的细胞、组织之间的相互作用深入理解。Rnascope®技术能够在保证组织形态的同时做到单细胞单分子水平的Rna原位检测。我们选择了50个免疫检查点和功能标记物。通过使用Rnascope®双染技术，展示了卵巢癌和肺癌中PD-L1和几个免疫标记物的定位。使用Rnascope®可见光单色棕染分析，我们检测了多种人类肿瘤中的12中免疫检查点标记物、14种免疫细胞标记物和24种免疫功能标记物，如细胞因子、趋化因子等。 详细信息请访问ACD官网www.acdbio.com。更多中文资料请关注中国官方微信号（ACD_China）咨询。
Genetic Analysis of Mammalian Cricadian Clocks
昼夜节律是一个适应的24小时的一天，我们的经验。一个历史性的概述，Takahashi开始他的演讲如何控制生物钟的基因在drosophi首次发现和克隆旅游所需的力量，以确定在小鼠时钟基因。他还介绍了实验，导致实现人体内的所有细胞都有一个生物钟，而不仅仅是在大脑中的细胞。在这部分讲座中，Takahashi解释说，视交叉上核（SCN）在大脑中产生一种昼夜节律的体温波动的体温反过来，信号到外周组织。热休克因子1是负责的信号分子之一通信温度信息和复位外周时钟。