从古希腊到21世纪，对多细胞生物中的再生能力的研究
Alejandro Sanchez-Alvarado (Stowers Institute for Medical Research) Part 1: History of Regeneration
Regeneration has fascinated philosophers and scientists since the beginning of history. The wide but uneven distribution of regenerative capacities among multicellular organisms is puzzling, and the permissive/inhibitory mechanisms regulating this attribute in animals remain a mystery. In the first part of this lecture, I will provide a general history of regeneration research from ancient Greece to the beginning of the 20th century. Key concepts will be introduced in their appropriate historical context, and many of the unanswered questions put forward by the problem of regeneration will be discussed.
Alejandro Sánchez Alvarado moved from the University of Utah to the Stowers Institute for Medical Research in 2011.
细胞囊泡萌芽与人类健康
Randy Schekman (Berkeley) Part 3: Human Diseases of Vesicle Budding
Human COPII genes are duplicated and some may have evolved specialized functions. Two rare human diseases affect the activity of one of two copies of Sar1 and the Sec23A subunit of the COPII coat. Anderson's disease results in the failure of enterocytes of the absorptive epithelium to secrete large lipoprotein particles called chylomicrons. Point mutations in one of two copies of Sar1 results in the accumulation of chylomicrons in the ER. CLSD, a rare craniofacial disorder likely due to the selective failure of secretion of certain connective tissue proteins such as collagen, is caused by a conservative amino acid substitution in Sec23A that blocks completion of COPII coat assembly. See more athttp://www.ibioseminars.org
GE：抗体药物开发中细胞培养基的筛选、优化与补料策略
在抗体药物的开发过程中如何选择合适的无血清细胞培养基，在实验结果未达到预期要求时，如何做培养基的优化，及feed-batch培养模式下，如何优化补料策略，来提升细胞状态、延长表达周期、提高抗体产量。
肌动蛋白与细胞运动
Illustration of the role of actin polymers in cell movement and its conservation across species. Movies of moving neutrophils, amoeba and fish keratinocytes or skin cells. (3 movies)
2016第二弹之细胞治疗中病毒载体的下游纯化
近年来，以CAR-T为代表的的特异性细胞免疫治疗为攻克癌症带来了一丝曙光，越来越多的创业公司及科研单位纷纷投身于该领域，方兴未艾！在细胞治疗整个流程中涉及细胞分选，培养，质粒及病毒包装纯化等，高品质的病毒载体在CAR-T疗法中至关重要。病毒载体的纯化方式可分为：超滤法，超高速离心法以及层析法等。其中，层析法对设备要求低，具有时间短，通量高，纯度高和易于放大的特点，是一种非常重要的病毒载体纯化手段。在本次报告中，我们将讨论如何利用不同的层析介质以及合理的衔接步骤对病毒载体进行纯化。
介绍干细胞及其来源的故事
What are stem cells, where do they come from, and what do we really know about them? An award-winning introduction to the world of stem cell research. Innovative hand-drawn animation, beautiful cell photography and documentary interviews capture the fascination and complexity of this cutting-edge area of science.
荧光免疫细胞化学检测法（ICC）- 完整
In this video, you will learn how to coat coverships, prepare cell culture plats, plate cells for ICC, fix, permeabilize, block and add a primary antibody to your plated cells, add a secondary antibody, wash the plated cells, double label and mount your coverslips for analysis.
荧光免疫细胞化学检测法（ICC）- 第1部分
In this video you will learn how to clean and coat coverslips, prepare cell culture plates, and plate cells for ICC.
荧光免疫细胞化学检测法（ICC）- 第2部分
In this video you will learn how to fix, permeabilize, block, and add primary antibody to your plated cells.