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CellSearch检测CTC--陈巍学基因(30)

欢迎来到【陈巍学基因】,我们这个节目,主要是为大家介绍基因组学,和临床分子诊断的最新技术进展。

今天,会和大家谈一下Jassen公司(强生公司)出品的CellSearch系统。它的主要应用是:检测循环肿瘤细胞,并对癌症给出预后信息。以下是课程内容概括:

1.什么是循环肿瘤细胞(CTC)及其“液体活检”的难点。

2. CellSearch系统的检测原理:(1)用微磁珠对CTC细胞进行富集;(2)用针对DNA的荧光染色剂“DAPI”进行染色,以排除红细胞;(3)区分白细胞和CTC细胞。

3.CellSearch系统实际操作的演示。

4.CTC检测,在癌症诊疗方面所起到的作用。

综上所述:CellSearch系统,是第一个标准化的、半自动化的,循环肿瘤细胞检测系统。它通过快速、精确地确定血液样本中的CTC细胞数量。可以帮助医生在整个治疗过程当中,提供准确的预后评估手段。

2015-12-14 课时:10分钟

RNAscope原位杂交技术助力新冠病毒检测及初步结果展示

RNAscope原位杂交技术助理新冠病毒检测及初步结果展示 1. RNAscope技术原理介绍 2. 在Zika,MERs-Cov,HIV等病毒研究中的应用实例 3. RNAscope应对新冠病毒研究的相关产品及结果展示

2019-04-30 课时:59分钟

石蜡包埋切片样本的RNAscope®检测操作步骤:棕色显色方法

对于石蜡包埋切片(FFPE)类型的样本,使用RNAscope®技术进行RNA原位定量检测分析,结果以棕色化学显色法显示。本视频对该实验进行了分步详细描述,供大家在实验开始时参考学习。

RNAscope®®原位定量专利技术由美国ACD公司(Advanced Cell Diagnostics, Inc., California, USA)开发,通过专利的双“Z”探针设计,使RNA原位杂交具有高度特异性、RNA单分子检测的敏感性并兼容自动化高通量分析,能够在单细胞水平同时定量多个RNA的表达。该视频由ACD公司制作,详细信息请访问ACD官网www.acdbio.com。更多中文资料请关注中国官方微信号(ACD_China)咨询。

2018-03-15 课时:2分钟

GE:常温稳定的核酸储存与检测体系

核酸样品越来越多冰箱不够用?核酸样品与检测试剂冷链运输费用昂贵?常温稳定的核酸储存与检测体系也许可以帮助您解决类似问题。核酸的常温采集,运输和储存方案可以帮您节约成本,简化流程。而采用稳定化保存的检测试剂,可无需冷链运输即可保持试剂活性,节约运输成本。

2016-04-05 课时:38分钟

如何做一个完美的Western Blot检测

In the western blot visual protocol video, you will learn how to prepare your samples before loading them into a gel, load a gel and separate the proteins through electrophoresis, transfer your proteins from the SDS-PAGE gel onto a PVDF or nitrocellulose membrane, block the membrane, stain in Ponceau red, add the primary and secondary antibodies, and visualize your protein of interest. Additional help can be found in the support section of http://www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.

2016-05-12 课时:15分钟

Western Blot 第5阶段:检测

Novus Biologicals Visual Protocols: In phase 5 of the western blot procedure, you will learn how to visualize your protein of interest that was probed with specific antibodies in the previous step. Here we utilize the electrochemiluminescent (ECL) to produce light where our antibodies are bound. This light is collected by film or a camera for later analysis. Additional help can be found in the support section of http://www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.

2016-05-13 课时:3分钟

荧光免疫细胞化学检测法(ICC)- 完整

In this video, you will learn how to coat coverships, prepare cell culture plats, plate cells for ICC, fix, permeabilize, block and add a primary antibody to your plated cells, add a secondary antibody, wash the plated cells, double label and mount your coverslips for analysis.

2016-05-18 课时:9分钟

荧光免疫细胞化学检测法(ICC)- 第1部分

In this video you will learn how to clean and coat coverslips, prepare cell culture plates, and plate cells for ICC.

2016-05-18 课时:3分钟

荧光免疫细胞化学检测法(ICC)- 第2部分

In this video you will learn how to fix, permeabilize, block, and add primary antibody to your plated cells.

2016-05-18 课时:4分钟

荧光免疫细胞化学检测法(ICC)- 第3部分

In this video you will learn how to add your secondary antibody, wash the plated cells, double label, and mount your coverslips onto slides for analysis.

2016-05-18 课时:3分钟