在实验室控制温度：加热
尽管很多实验分析都是在常温下进行的，但是对于某些实验或者实验的一部分经常需要进行温度的调控也是比较常见的。本短片讨论了不同的原因以及研究人员希望保持物品温暖所需要的温度。例如，有些时候，细胞需要在环境温度接近于体温的时候进行培养（37度）；蛋白质结构需要被修饰（高于56度）或者试剂和溶液需要被加热（约100度）。还将讨论如何采用合适的方式去操作温度计来测量搅动溶液的温度。因为在实验室中如何调节温度并非总是很清楚，本视频将会阐述在每种条件下会选择哪一种仪器装备。
对头足纲动物视觉感知机制的探索 - Roger Hanlon P2
本视频由科普中国和生物医学大讲堂出品
Roger Hanlon (MBL) Part 2: Exploring Mechanisms of Visual Perception
Hanlon introduces the amazing adaptive coloration of cephalopods. He uses video and still photography to showcase their ability to rapidly change color, pattern and skin texture with fine control and a diversity of appearances, to produce camouflage or to send signals. He argues that all camouflage patterns in nature can be grouped into three types. In part 2, Hanlon shows us results from his lab that make a convincing case that the rapid adaptive coloration of cephalopods is controlled by their visual system; quite impressive for a color-blind animal! Part 3 focuses on the unique skin of cephalopods including the system of pigments and reflectors that allows it to quickly change to any hue and contrast, and the papillae musculature that allows the skin to deform and create multiple 3D textures.
控制声乐学习行为的大脑通路 - Erich Jarvis P1
本视频由科普中国和生物医学大讲堂出品
Erich Jarvis (Duke/HHMI) Part 1: Convergent behavior and brain pathways
In Part 1, Jarvis explains that vocal learning is the ability to hear a sound and repeat it. Only 5 groups of mammals (including humans) and 3 groups of birds (parrots, hummingbirds and songbirds) are capable of vocal learning. Jarvis and his lab members imaged changes in gene expression in bird's brains after singing. They found that hummingbirds, songbirds and parrots each have pathways in specific areas of the brain that are not found in non-vocal learning birds. Interestingly, analogous networks exist in the human brain but not in non-vocal learning monkeys.
In Part 2, Jarvis proposes a mechanism by which vocal learning may have evolved. He suggests that the brain areas that control vocal learning are the result of a duplication of a pre-existing neural circuit that controls motor movement. A similar duplication event may have occurred during the evolution of humans with the result that both humans and Snowball, a cockatoo, can sing and dance to a beat!
In Jarvis' third talk, he demonstrates that the brain pathways necessary for vocal learning are associated with the expression of particular axonal guidance genes. He also proposes that the evolutionary events responsible for the development of vocal learning may be a general mechanism for the development of other complex behavioral traits.
控制老化的基因 - Cynthia Kenyon P1
本视频由科普中国和生物医学大讲堂出品
Cynthia Kenyon (UCSF) Part 1: Genes that Control Aging
Once it was thought that aging was just a random and haphazard process. Instead, the rate of aging turns out to be subject to regulation by transcription factors that respond to hormones and other signals. In the nematode C. elegans, in which many key discoveries about aging were first made, the aging process is subject to regulation by food intake, sensory perception, and signals from the reproductive system. Changing genes and cells that affect aging can lengthen lifespan by six fold, and can also delay age-related disease, such as the growth of tumors.
限制性内切酶消化
限制性酶或内切酶会识别并切开DNA上的某个特定序列。这些酶在细菌中天然存在，用来抵御感染细菌的病毒-噬菌体的攻击。细菌中的限制性内切酶会切开入侵噬菌体的DNA，而细菌本身的DNA由于有甲基化，将不会受到影响。
本短片讲述了限制性内切酶的基本原理：内切酶是如何命名的，已有的酶切位点和突出端类型。并将讲解常用的限制性内切酶消化的逐步操作过程，包括所需组分，添加各组分到混合物中的顺序，以及常用的消化温度和时间。还将提及灭活限制性内切酶来抑制非特异性活性的重要性。短片还给出了操作多酶消化及酶切中使用对照的小技巧。
控制衰老的基因电路
In the early 1990s, most scientists did not think that aging was subject to active regulation by the genes. Exciting results from Dr. Kenyon's lab, however, showed that a single mutation in the daf-2 gene caused the tiny roundworm C. elegans to live twice as long as normal. This gene encodes a hormone receptor that regulates lifespan not only in worms, but in flies, mammals and possibly humans as well.
寻找控制生长发育的基因
Eric Wieschaus and Christiane Nusslein-Volhard wanted to understand which genes regulated embryonic development in Drosophila. It took them 2 years to design their experiment and only a few months to execute. A combination of good design and good luck allowed them to start with 40,000 flies and identify just 139 genes necessary for embryonic development.
DNA的半保守复制
1953年，Watson和Crick提出了，DNA的双螺旋结构和它的半保守复制方式。在当时，这种复制方式并没有被普遍接受。本短片中，梅塞尔森回忆了，他是如何遇见Frank Stahl,为什么要计划和执行这个著名的实验，去证明DNA的半保留复制方式。
GE：2016第一弹之蛋白样品制备
蛋白样品制备是进行样品分析前的第一步，制备的质量对于成功进行蛋白分析来说至关重要。本次内容主要从蛋白收集提取、亲和富集、准确定量到脱盐除杂进行讲解，努力为在蛋白质分析研究领域入门或探索阶段的科研人员提供完整的解决方案。
Western Blot 第1阶段：样品制备
Novus Biologicals Visual Protocols: In phase 1 of the western blot procedure, you will learn how to prepare your samples before loading them into a gel. Here we isolate protein from cultured cells, quantify total protein concentrations with a BCA assay, add loading buffer to the sample, and heat the sample. Additional help can be found in the support section of http://www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.