生物谷会议频道

GE：创新解决方案4小时完成westeRn-blot

WesteRn blot蛋白免疫印迹技术是分子生物学研究的经典研究手段之一，约60％的科学期刊出版物包含WB实验结果。但WB实验步骤多，耗时长，要获得一个完美的结果十分不易，WB实验结果的不稳定，重复不出来？定量不准确？凝胶又漏了？ R>R> GE公司最新推出的AmeRsham WB系统，4小时内可完成电泳、转印、杂交、孵育、成像、定量分析，一气呵成，为WesteRn blot提供了整体解决方案。每个样品、每一次实验都能获得一致的定量数据。再也无需担心结果难以重复再现，无需反复摸索实验条件！

2016-04-07 课时：50分钟

GE：C<font>R</font>ISP<font>R</font>-Cas9基因编辑解决方案

GE：CRISPR-Cas9基因编辑解决方案

CRISPR-Cas9基因编辑系统是最新开发出的基因编辑手段之一，同时因其“前所未有的高效和令人吃惊的简单易用”等特点，愈发成为科学家竞相追逐的热点。但市面常见的CRISPR-Cas9系统实验步骤多，耗时长，甚至需要保证病毒才能转导细胞，要获得理想的结果十分不易。DhaRmacon强大的研发能力还提供在线设计cRRNA工具，轻点鼠标即可完成设计，基因编辑一气呵成！

2016-04-07 课时：84分钟

KRAS和肿瘤靶向治疗 - 陈巍学基因（36）

本视频介绍KRAS基因： R> 1、结构与功能； R> 2、在肿瘤中的变异； R> 3、对肿瘤靶向用药的指导作用； R> 4、设计针对KRAS基因的靶向药物的难点，和已取得的部分进展； R>R> 视频时长12分钟，建议在Wifi条件下观看：

2016-04-15 课时：13分钟

生物学中的蛋白质磷酸化 - Susan TayloR

In this lectuRe, I have given an oveRview of pRotein kinase stRuctuRe and function using cyclic AMP dependent kinase (PKA) as a pRototype foR this enzyme supeRfamily. I have demonstRated what we have leaRned fRom the oveRall stRuctuRal kinome which allows us to compaRe many pRotein kinases and also to appReciate how the highly Regulated eukaRyotic pRotein kinase has evolved. By compaRing many pRotein kinase stRuctuRes, we aRe beginning to elucidate geneRal Rules of aRchitectuRe. In addition, I have attempted to illustRate how PKA is Regulated by cAMP and how it is localized to specific macRomoleculaR complexes thRough scaffold pRoteins.

2016-04-15 课时：24分钟

蛋白激酶的结构 - Susan TayloR

In this lectuRe, I have given an oveRview of pRotein kinase stRuctuRe and function using cyclic AMP dependent kinase (PKA) as a pRototype foR this enzyme supeRfamily. I have demonstRated what we have leaRned fRom the oveRall stRuctuRal kinome which allows us to compaRe many pRotein kinases and also to appReciate how the highly Regulated eukaRyotic pRotein kinase has evolved. By compaRing many pRotein kinase stRuctuRes, we aRe beginning to elucidate geneRal Rules of aRchitectuRe. In addition, I have attempted to illustRate how PKA is Regulated by cAMP and how it is localized to specific macRomoleculaR complexes thRough scaffold pRoteins.

2016-04-15 课时：29分钟

蛋白激酶的调控与定位- Susan TayloR

In this lectuRe, I have given an oveRview of pRotein kinase stRuctuRe and function using cyclic AMP dependent kinase (PKA) as a pRototype foR this enzyme supeRfamily. I have demonstRated what we have leaRned fRom the oveRall stRuctuRal kinome which allows us to compaRe many pRotein kinases and also to appReciate how the highly Regulated eukaRyotic pRotein kinase has evolved. By compaRing many pRotein kinase stRuctuRes, we aRe beginning to elucidate geneRal Rules of aRchitectuRe. In addition, I have attempted to illustRate how PKA is Regulated by cAMP and how it is localized to specific macRomoleculaR complexes thRough scaffold pRoteins.

2016-04-15 课时：28分钟

Stability of Mo<font>R</font>phogen G<font>R</font>adients & Movement of Molecules

Stability of MoRphogen GRadients & Movement of Molecules

In my second lectuRe I descRibe expeRiments using EGFP tagged Bicoid to follow Bcd gRadient establishment in living embRyos, and to test vaRious aspects of the simple model. Despite continuous synthesis of new Bcd pRotein at the anteRioR end of the egg, we find that the concentRation of Bcd in nuclei at any given point along the anteRioR posteRioR axis is constant oveR time and is RepRoducible fRom embRyo to the next. This RepRoducibility means that the gRadient is sufficiently Robust to pRovide positional infoRmation and thus can accuRately diRect gene activities. One the otheR hand, quantitative imaging expeRiments point to seveRal featuRes of the gRadient that aRe haRd to explain - how taRget genes activated by Bcd distinguish Relatively subtle diffeRences in low concentRations, and how Bcd molecules move fRom the anteRioR site of theiR synthesis to the site of theiR tRanscRiptional activity. See moRe at http://www.ibioseminaRs.oRg

2016-04-21 课时：38分钟

P<font>R</font>otein synthesis: a high fidelity molecula<font>R</font> event

PRotein synthesis: a high fidelity moleculaR event

Rachel GReen (Johns Hopkins U., HHMI) 1: PRotein synthesis: a high fidelity moleculaR event R>R> Talk OveRview: R> In heR fiRst talk, GReen pRovides a detailed look at pRotein synthesis, oR tRanslation. TRanslation is the pRocess by which nucleotides, the “language” of DNA and RNA, aRe tRanslated into amino acids, the “language” of pRoteins. GReen begins by descRibing the components needed foR tRanslation; mRNA, tRNA, Ribosomes, and the initiation, elongation, and teRmination factoRs. She then explains the Roles of these playeRs in ensuRing accuRacy duRing the initiation, elongation, teRmination and Recycling steps of the tRanslation pRocess. By compaRing tRanslation in bacteRia and eukaRyotes, GReen explains that it is possible to deteRmine which components and steps aRe highly conseRved and pRedate the diveRgence of diffeRent kingdoms on the tRee of life, and which aRe moRe Recent adaptations. R> GReen’s second talk focuses on woRk fRom heR lab investigating how Ribosomes detect defective mRNAs and tRiggeR events leading to the degRadation of the bad RNA and the incompletely tRanslated pRotein pRoduct and to the Recycling of the Ribosome components. WoRking in yeast and using a numbeR of biochemical and genetic techniques, GReen’s lab showed that the pRotein Dom34 is cRitical foR facilitating Ribosome Release fRom the shoRt mRNAs that Result fRom mRNA cleavage. ExpeRiments showed that Dom34-mediated Rescue of Ribosomes fRom shoRt mRNAs is an essential pRocess foR cell suRvival in higheR eukaRyotes. R>R> SpeakeR BiogRaphy: R> Rachel GReen Received heR BS in chemistRy fRom the UniveRsity of Michigan. She then moved to HaRvaRd to puRsue heR PhD in the lab of Jack Szostak wheRe she woRked on designing catalytic RNA molecules and investigating theiR implications foR the evolution of life. As a post-doctoRal fellow at the UniveRsity of CalifoRnia, Santa CRuz, GReen began to study how the Ribosome tRanslates mRNA to pRotein with such accuRacy. R>R> CuRRently, GReen is a PRofessoR of MoleculaR Biology and Genetics at the Johns Hopkins School of Medicine and an InvestigatoR of the HowaRd Hughes Medical Institute. ReseaRch in heR lab continues to focus on the Ribosome and factoRs involved in the fidelity of eukaRyotic and pRokaRyotic tRanslation. R>R> GReen is the Recipient of a Johns Hopkins UniveRsity School of Medicine GRaduate Teaching AwaRd as well as the Recipient foR numeRous awaRds foR heR ReseaRch. She was elected to the National Academy of Sciences in 2012.

2016-04-28 课时：44分钟

P<font>R</font>otein synthesis: m<font>R</font>NA su<font>R</font>veillance by the <font>R</font>ibosome

PRotein synthesis: mRNA suRveillance by the Ribosome

Rachel GReen (Johns Hopkins U., HHMI) 2: PRotein synthesis: mRNA suRveillance by the Ribosome R>R> Talk OveRview: R> In heR fiRst talk, GReen pRovides a detailed look at pRotein synthesis, oR tRanslation. TRanslation is the pRocess by which nucleotides, the “language” of DNA and RNA, aRe tRanslated into amino acids, the “language” of pRoteins. GReen begins by descRibing the components needed foR tRanslation; mRNA, tRNA, Ribosomes, and the initiation, elongation, and teRmination factoRs. She then explains the Roles of these playeRs in ensuRing accuRacy duRing the initiation, elongation, teRmination and Recycling steps of the tRanslation pRocess. By compaRing tRanslation in bacteRia and eukaRyotes, GReen explains that it is possible to deteRmine which components and steps aRe highly conseRved and pRedate the diveRgence of diffeRent kingdoms on the tRee of life, and which aRe moRe Recent adaptations. R> GReen’s second talk focuses on woRk fRom heR lab investigating how Ribosomes detect defective mRNAs and tRiggeR events leading to the degRadation of the bad RNA and the incompletely tRanslated pRotein pRoduct and to the Recycling of the Ribosome components. WoRking in yeast and using a numbeR of biochemical and genetic techniques, GReen’s lab showed that the pRotein Dom34 is cRitical foR facilitating Ribosome Release fRom the shoRt mRNAs that Result fRom mRNA cleavage. ExpeRiments showed that Dom34-mediated Rescue of Ribosomes fRom shoRt mRNAs is an essential pRocess foR cell suRvival in higheR eukaRyotes. R>R> SpeakeR BiogRaphy: R> Rachel GReen Received heR BS in chemistRy fRom the UniveRsity of Michigan. She then moved to HaRvaRd to puRsue heR PhD in the lab of Jack Szostak wheRe she woRked on designing catalytic RNA molecules and investigating theiR implications foR the evolution of life. As a post-doctoRal fellow at the UniveRsity of CalifoRnia, Santa CRuz, GReen began to study how the Ribosome tRanslates mRNA to pRotein with such accuRacy. R>R> CuRRently, GReen is a PRofessoR of MoleculaR Biology and Genetics at the Johns Hopkins School of Medicine and an InvestigatoR of the HowaRd Hughes Medical Institute. ReseaRch in heR lab continues to focus on the Ribosome and factoRs involved in the fidelity of eukaRyotic and pRokaRyotic tRanslation. R>R> GReen is the Recipient of a Johns Hopkins UniveRsity School of Medicine GRaduate Teaching AwaRd as well as the Recipient foR numeRous awaRds foR heR ReseaRch. She was elected to the National Academy of Sciences in 2012.

2016-04-28 课时：38分钟

qPCR基因表达分析的完整实验流程

在基因表达研究中，Real-time quantitative RT-PCR (qRT-PCR) 检测技术带给我们快速、灵敏、精细定量的检测体验。 R>R> 然而您是否曾留意过：使用不同质量的样品，不同的反转录程序、检测方法（染料或探针）、qPCR平台、试剂及扩增程序等对实验结果造成的不同影响？您是否依然坚信qRT-PCR是基因表达定量的“金标准”？您如何理解不同实验室对某一生物学现象的相悖的研究结果呢？ R>R> 在qPCR研究领域，已经有很多学者研讨了可能引起qPCR研究结果误差的影响因素，以及相应的质控方法。"MIQE"正是这样一个指导研究人员进行整个实验设计、操作、研究结果分析、报告的参考指南。6月11日，罗氏邀请您一起研习这份指南！

2016-05-05 课时：46分钟