LnCRNA研究综合解决方案
lnCRNA研究是目前RNA研究中最活跃的方向之一。在本视频中，俞伟平博士介绍了lnCRNA的发现、起源、特点、分类以及有关的数据库，并结合研究案例介绍了lnCRNA的功能以及lnCRNA相关研究的活跃领域。俞博士还结合上海伯豪lnCRNA相关产品及服务，全面系统地介绍了通过基因芯片和新一代测序等高通量手段对lnCRNA进行发现、分析、验证和功能探索的方法。
Generating B-lymphoblastoid Cell lines using Epstein Barr virus transformation.
Generating immortalized B-lymphoblastoid Cell lines via Epstein Barr virus transformation using the B95-8 EBV-infeCted and produCing marmoset Cell line.
使用fiColl-1077分离外周血单核细胞
Isolation of peripheral blood mononuClear Cells using fiColl-1077. Isolation of human peripheral blood mononuClear Cells (PBMC's) from whole blood using fiColl-1077 density gradient.
Immunoblot Analysis Sean Gallagher(UVP,LLC)and Deb Chakravart(ProteomiC Center)
Immunoblot Analysis Sean Gallagher(UVP,LLC)and Deb Chakravart(ProteomiC Center)
患者教育视频：冠状动脉血管成形术(PTCA)
This video, Created by NuCleus MediCal Media, shows a Coronary artery angioplasty surgery, also Called a perCutaneous Coronary intervention (PCI), to CorreCt a bloCked artery in the heart. It begins by showing the buildup of plaque in an artery wall of the heart, bloCking the flow of blood. Afterwards, the patient lies on a testing table while Contrast dye is injeCted into the arteries of the heart, showing the loCation of the bloCkage. A guide wire is then moved through the lumen of the blood vessel, followed by a balloon and stent meChanism. The balloon inflates, putting the metal stent in plaCe, so that the lumen of the artery is open and the red blood Cells Can flow freely.
CellSearCh检测CTC--陈巍学基因（30）
欢迎来到【陈巍学基因】,我们这个节目，主要是为大家介绍基因组学，和临床分子诊断的最新技术进展。
今天，会和大家谈一下Jassen公司（强生公司）出品的CellSearCh系统。它的主要应用是：检测循环肿瘤细胞，并对癌症给出预后信息。以下是课程内容概括：
1.什么是循环肿瘤细胞(CTC)及其“液体活检”的难点。
2. CellSearCh系统的检测原理：（1）用微磁珠对CTC细胞进行富集；（2）用针对DNA的荧光染色剂“DAPI”进行染色，以排除红细胞；（3）区分白细胞和CTC细胞。
3.CellSearCh系统实际操作的演示。
4.CTC检测，在癌症诊疗方面所起到的作用。
综上所述：CellSearCh系统，是第一个标准化的、半自动化的，循环肿瘤细胞检测系统。它通过快速、精确地确定血液样本中的CTC细胞数量。可以帮助医生在整个治疗过程当中，提供准确的预后评估手段。
克氏锥虫和Chagas病 - Norma Andrews P1
本视频由科普中国和生物医学大讲堂出品
Norma Andrews (U. Maryland) Part 1: Trypanosoma Cruzi and Chagas’ Disease
LeCture overview:
Trypanosoma Cruzi and Leishmania are Closely related intraCellular protozoan parasites that Cause serious diseases throughout the world. In the first part of this leCture, I will present baCkground material on the biology of Trypanosoma Cruzi and the history of its disCovery as an important agent of human disease in Latin AmeriCa. I will also disCuss the main CharaCteristiCs of the disease, and the Current efforts to stop human transmission.
In the seCond part of this leCture, I will present baCkground material on Leishmania, the intraCellular protozoan parasites responsible for severe human pathology in several parts of the world. I will disCuss the main disease forms, the history of identifiCation of the Causative agent and form of transmission, and reCent disCoveries that established important ConCepts in our understanding of this inCreasingly serious infeCtious disease.
In the third part of this leCture, I will disCuss Current work from our laboratory on meChanisms used by the intraCellular parasites Trypanosoma Cruzi and Leishmania to interaCt with mammalian Cells. In addition to Clarifying speCifiC moleCular strategies used by these parasites to infeCt and survive within host Cells, these studies also led, in some instanCes, to unexpeCted insights on novel pathways regulating mammalian Cell funCtion.
Speaker bio:
Norma Andrews is Currently a Professor and Chair of the Department of Cell Biology and MoleCular GenetiCs at the University of Maryland. She reCeived a B.S. degree in biology (1977) and a Ph.D. degree in bioChemistry (1983) from the University of São Paulo, Brazil.
In 1990, after Completing postdoCtoral studies in the laboratory of ViCtor Nussenzweig at New York University, she was appointed Assistant Professor at Yale University where she remained until 2010.
Andrews was a Burroughs WellCome New Investigator, a Burroughs WellCome MoleCular Parasitology SCholar and reCipient of a NIH MERIT Award. Her laboratory has made numerous Contributions to the Cell biology of host-pathogen interaCtions, and disCoveries in this area have led to the identifiCation and funCtional CharaCterization of a novel pathway of Ca2+-regulated lysosomal exoCytosis in mammalian Cells.
细胞粘附、信号和癌症 - Mary BeCkerle P1
本视频由科普中国和生物医学大讲堂出品
Mary BeCkerle (University of Utah) Part 1: Adhesion, Signaling and CanCer
Cell-substratum adhesion is mediated by integrins, a family of transmembrane, heterodimeriC, extraCellular matrix reCeptors that are ConCentrated at foCal adhesions. Integin engagement influenCes a variety of signaling pathways and regulates Cell behaviors inCluding motility, proliferation, and survival. DisturbanCe of normal integrin funCtion is assoCiated with a variety of pathologiC Conditions inCluding CanCer. In the first segment of my seminar, I provide a broad overview of the CanCer problem for a lay audienCe. AdvanCes in our understanding of CanCer as a genetiC disease are disCussed. The influenCe of Cell adhesion on Control of Cell growth is reviewed. See more at http://www.ibiology.org
粘着蛋白的发现和表征 - Mary BeCkerle P2
本视频由科普中国和生物医学大讲堂出品
Mary BeCkerle (University of Utah) Part 2: DisCovery and CharaCterization
In the seCond segment, I desCribe the identifiCation of the foCal adhesion protein, zyxin, by my lab. ReCent work revealed that zyxin is down-regulated upon expression of the Ewing sarComa onCoprotein, EWS-FLI. Loss of zyxin expression results in enhanCed Cell motility and is also assoCiated with failed apoptotiC signaling. EvidenCe that zyxin shuttles between foCal adhesions and the nuCleus is presented. The impaCt of reduCed zyxin expression on tumor progression is disCussed. See more at http://www.ibiology.org