如何解冻被冷冻保存的外周血单核细胞或细胞系。
Thawing of cryopreserved PBMC's or cell lines. Thawing procedure for peripheral blood mononuclear cells (PBMC's) or cell lines cryopreserved in liquid nitrogen (LN2).
2015-12-08 课时：3分钟
使用ficoll-1077分离外周血单核细胞
Isolation of peripheral blood mononuclear cells using ficoll-1077. Isolation of human peripheral blood mononuclear cells (PBMC's) from whole blood using ficoll-1077 density gradient.
2015-12-08 课时：10分钟
建立EB病毒转化B淋巴母细胞系
Generating B-lymphoblastoid cell lines using Epstein Barr virus transformation. Generating immortalized B-lymphoblastoid cell lines via Epstein Barr virus transformation using the B95-8 EBV-infected and producing marmoset cell line.
2015-12-08 课时：7分钟
