说服选民通过保护干细胞研究的提案
The story of how Morrison and his colleagues convinced Michigan voters to pass Proposal 2 and protect stem cell research in the state.
将RNAscope®技术应用于病毒学的研究
Advanced Cell Diagnostics邀请了美国加利福尼亚大学（Davis）微生物、免疫及病理学系教授Patricia Pesavento，为大家介绍应用RNAscope®技术在研究新发现的病毒及致病病因、病理方面的研究。引起疾病的病毒可以跨越种族感染，通过测序发现新型病毒，利用RNAscope®进行病毒病理学分析，以准确判断疾病及复杂的宿主病毒相互影响。在这一讲座中，Pesavento教授以乳头瘤病毒等举例，对病毒的潜伏、致瘤和急性裂解细胞分析，分享了病毒学的研究进展。
RNAscope®技术可以针对任一病毒（或亚型）序列设计特异性探针，通过原位定量检测获得病毒感染、潜伏及与宿主反应的研究信息。详细信息请访问ACD官网www.acdbio.com。更多中文资料请关注中国官方微信号（ACD_China）咨询。
如何在斑马鱼完整胚胎样本中使用RNAscope®技术进行研究
整胚原位杂交是在完整胚胎和组织中研究基因时空表达模式的有力工具。但现有的实验方法无法准确的直接检测RNA，而且操作耗时，结果和蛋白表达水平不一致。新一代原位定量杂交技术RNAscope®可以在斑马鱼整胚上实现快速高效、精准定量、特异性的多重RNA原位检测。该webinar由美国ACD公司(Advanced Cell Diagnostics, Inc., California, USA)邀请德国Münster大学细胞生物学研究所Erez Raz教授实验室两位研究员Azadeh Paksa 和 Theresa Gros介绍他们利用RNAscope®技术实现斑马鱼整胚上同时原位检测3个RNA分子进行三维荧光分析。详细介绍了实验操作过程，如何优化条件，降低信噪比以及RNAscope®技术相比传统原位杂交技术的绝对优势。
ACD公司提供RNAscope®原位定量杂交专利技术和产品，详细信息请访问ACD官网www.acdbio.com。更多中文资料请关注中国官方微信号（ACD_China）咨询。
文章题目: Simultaneous high-resolution detection of multiple transcripts combined with localization of proteins in whole-mount embryos. Gross-Thebing T, Paksa A, Raz E. BMC Biol. 2014 Aug 15;12(1):55.
人类彩色视觉及其变异性的研究
Jeremy Nathans (Johns Hopkins) Part 2: Human Color Vision and its Variations
In this set of lectures, Jeremy Nathans explores the molecular mechanisms within the retina that mediate the first steps in vision. The second lecture focuses on the photoreceptors that mediate human color vision and the molecular basis for the common inherited anomalies of color vision. See more at http://www.ibioseminars.org
细胞大小究竟如何调控细胞增殖
Martin Raff (UCL) Part 1: Regulation of Cell Size
The size of an organ or organism depends mainly on the sizes and numbers of the cells it contains. In the first segment of my talk, I describe our work on cell size control in cultures of purified rat Schwann cells. Most proliferating cells grow before they divide, but it is not known how growth and division are co-ordinated to ensure that cells divide at an appropriate size. We have found that extracellular signals can control cell growth and cell-cycle progression separately and that the size of Schwann cells at division depends on the signalled rates of both cell growth and cell-cycle progression, rather than on a cell-size checkpoint that monitors cell size.
细胞数目究竟如何调控细胞增殖
Martin Raff (UCL) Part 2: Cell Number Control
In the second segment of my talk, I describe our work on cell number control in the rat oligodendrocyte cell lineage. Cell numbers depend on controls on both cell death and cell proliferation. We have found that oligodendrocytes are normally overproduced and kill themselves in large numbers in a competition for survival signals on the surface of the axons that the oligodendrocytes myelinate. Most differentiated cells, including oligodendrocytes, develop from dividing precursor cells that divide a limited number of times before they terminally differentiate, but it is not known what stops cell division and initiates differentiation. We have found that oligodendrocyte precursor cells have an intrinsic timing mechanism that helps determine when they stop dividing and differentiate. See more at http://www.ibioseminars.org
研究寄生虫的质体，提供药物开发的新靶点
David Roos (U Penn) Part 2: The apicomplexan plastid
Antibiotics are effective because they kill bacteria without harming humans and other eukaryotes (organisms with cells that contain nuclei). So why are the eukaryotic parasites responsible for malaria and toxoplasmosis killed by drugs like clindamycin? Multidisciplinary studies integrating molecular genetics, cell biology, biochemistry, pharmacology and computational genomics reveal that such drugs target an unusual organelle. The "apicoplast" was acquired when an ancestral organism 'ate' a eukaryotic alga, and retained the algal plastid -- a relative of plant chloroplasts derived from a bacterial ancestor. Although no longer photosynthetic, the apicoplast is essential for parasite survival, providing new targets for drug development. See more at http://www.ibioseminars.org
从古希腊到21世纪，对多细胞生物中的再生能力的研究
Alejandro Sanchez-Alvarado (Stowers Institute for Medical Research) Part 1: History of Regeneration
Regeneration has fascinated philosophers and scientists since the beginning of history. The wide but uneven distribution of regenerative capacities among multicellular organisms is puzzling, and the permissive/inhibitory mechanisms regulating this attribute in animals remain a mystery. In the first part of this lecture, I will provide a general history of regeneration research from ancient Greece to the beginning of the 20th century. Key concepts will be introduced in their appropriate historical context, and many of the unanswered questions put forward by the problem of regeneration will be discussed.
Alejandro Sánchez Alvarado moved from the University of Utah to the Stowers Institute for Medical Research in 2011.
酵母蛋白分泌途径的研究
Randy Schekman (Berkeley) Part 1: Studying Protein Secretion in Yeast
Protein secretion is executed by a cellular pathway involving the delivery of membrane and soluble secretory proteins in vesicles that capture newly-synthesized proteins assembled in the endoplasmic reticulum (ER) and sorted in the Golgi apparatus. Vesicles fuse with the plasma membrane resulting in the discharge of soluble molecules to the cell exterior and integration of vesicle membrane proteins and lipids in the cell surface. Baker's yeast cells grow by vesicle fusion and secretion at the tip of the daughter bud. A genetic dissection of this process was performed with temperature sensitive conditional mutants blocked at one of several stations in the secretory pathway. See more athttp://www.ibioseminars.org
单分子分析方法研究运动蛋白
Molecular motor proteins are fascinating enzymes that power much of the movement performed by living organisms. In the first part of this lecture, I will provide an overview of the motors that move along cytoskeletal tracks (kinesin and dynein which move along microtubules and myosin which moves along actin). The main focus of this lecture is on how motor proteins work. How does a nanoscale protein convert energy from ATP hydrolysis into unidirectional motion and force production? What tools do we have at our disposal to study them? The first part of the lecture will focus on these questions for kinesin (a microtubule-based motor) and myosin (an actin-based motor), since they have been the subject of extensive studies and good models for their mechanisms have emerged. I conclude by discussing the importance of understanding motor proteins for human disease, in particular illustrating a recent biotechnology effort from Cytokinetics, Inc. to develop drugs that activate cardiac myosins to improve cardiac contractility in patients suffering from heart failure. The first part of the lecture is directed to a general audience or a beginning graduate class.
In the second part of this lecture, I will discuss our laboratories current work on the mechanism of movement by dynein, a motor protein about which we still know very little. This is a research story in progress, where some advances have been made. However, much remains to be done in order to understand how this motor works.
The third (last) part of the lecture is on mitosis, the process by which chromosomes are aligned and then segregated during cell division. I will describe our efforts to find new proteins that are important for mitosis through a high throughput RNAi screen. I will discuss how we technically executed the screen and then focus on new proteins that are we discovered that are involved in generating the microtubules that compose the mitotic spindle. I also discuss the medical importance of studying mitosis, including the development of drugs targeted to mitotic motor proteins, which are currently undergoing testing in clinical trials.