微滴式数字PCR检测血浆中非细胞游离dNA的EGFR突变
2013年作为PCR技术诞生30周年，在美丽的敦煌，2013第一届数字PCR前沿应用研讨会围绕PCR技术以及新一代的微滴式数字PCR在肿瘤标记分子领域的应用展开研讨，海内外知名的相关领域卓有建树的科学家采用大会报告以及专题讨论的形式，结合国际上转化医学研究的前沿动态与发展趋势，为大家带来一场生命科学的学术盛宴。
三个不同dPCR平台用于dNA拷贝数测量的比较研究
2013第一届数字PCR前沿应用研讨会于2013年8月20日（20日注册）~8月23日在敦煌（敦煌山庄）召开，2013年作为PCR技术诞生30周年，在美丽的敦煌，本次论坛将围绕PCR技术以及新一代的微滴式数字PCR在肿瘤标记分子领域的应用展开研讨，海内外知名的相关领域卓有建树的科学家采用大会报告以及专题讨论的形式，结合国际上转化医学研究的前沿动态与发展趋势，为大家带来一场生命科学的学术盛宴。
PHd2沉默提高脂肪来源干细胞移植...
心肌梗死是一种严重的心血管疾病，发病率和病死率高，严重威胁人类健康。大量心肌细胞的不断丢失是心肌梗死发展为心力衰竭并最终导致死亡的重要病理机制。由于成年心肌自我再生能力极弱，挽救和修复受损的心脏组织是治疗心肌梗死的目标。干细胞治疗心肌梗死成为近年的研究热点和未来的发展方向。目前全球已有上百个干细胞治疗心肌梗死的临床试验，这些结果虽然证明干细胞移植有一定疗效，但其效果并不理想。其中一个重要原因是干细胞在缺血缺氧、超氧应激的移植微环境下的存活率及功能低下，这也是本领域亟待解决的难题。
Regenerative medicine for brain and nerve repair
We isolated and propagated neural stem cells from the exposed brain tissue of the patients with open brain trauma, and then implanted neural stem cells with MRI-guided stereotactic device for the patients. Within 2-years follow-ups, the patients were investigated for functional recovery. Contrast to the case control group, implantation of neural stem cells was associated with a significant improvement in patient's neurological function. Investigations of stem cell therapy have required analysis of the fate and migration of implanted neural stem cells. Here, We demonstrate the feasibility of labeling human neural stem cells and retinal stem cells with nanoparticle and tracking of implanted cells in monkey and human central nervous system (CNS). This data demonstrates the possibility of stem cell therapy in CNS and collectively provide necessary foundation for overcoming challenges to the enhancement of translational regenerative medicine of brain and optic nerve injury.
Study the pathological features of diseases using induced pluripotent stem cells derived form patient's somatic cells
The limited experimental access to disease-affected human tissues has severely impeded the elucidating of molecular mechanisms underlying disease development. Generation of induced pluripotent stem cells (iPSCs) by over-expression of defined transcription factors in somatic cells, in particular in those from patient somatic cells, presents an attractive and promising approach to model the early stages of diseases in vitro and to screen novel biomarkers as well as therapeutic medicines. Recently, many research groups have independently reported that patient-specific iPSC-derived cells recapitulated multiple features of pathological events of a particular disease, offering experimental evidence of utilizing patient-specific iPSCs to model diseases and reevaluate the current therapies. We have derived iPSC lines using somatic cells of patients suffering from Klinefelter's Syndrome (KS) and Alzheimer's disease (Ad) and explored the possibility to use these iPSC lines to recapitulate the pathological features of the diseases. Our results show that patient's specific iPSC lines provide good opportunity to study the development and treatment of diseases.
sRNA Induces the Large-scale Transdetermination of Mesenchymal Stem Cells into Hematopoietic Stem Cells in Human.
Mesenchymal stem cells (MSCs) can differentiate into cells of bone, endothelium, adipose tissue, cartilage, muscle, and brain. However, whether they can transdeterminate into hematopoietic stem cells (HSCs) remains unsolved. We report here that a subpopulation of human MSCs that are Cd44+,Cd29+, Cd105+, Cd166+,Cd133-,Cd34- could differentiate into hematopoietic stem cells (Cd150+/Cd133+/Cd34+) and their descending blood cells in vitro, when transfected with new endogenous shRNAs The sRNA was high-effectively delivered into MSCs by a novel peptide means. These induced MSC-HSCs could form different types of hematopoietic colonies as nature-occurring HSCs did. Upon transplantation into sublethally irradiated NOd/SCId mice, these MSC-HSCs engrafted and differentiated into all hematopoietic lineages such as erythrocytes, lymphocytes, myelocytes and thrombocyte. More importantly, these induced HSCs could successfully engraft and effectively function in patients with severe aplastic anemia. Furthermore, we demonstrated the first evidence that the transdetermination of MSCs was induced by acetylation of histone proteins and activation of many transcriptional factors. Together, our findings identify the sRNAs that dictates a directed differentiation of MSCs toward HSCs and open up a new source for HSCs used for the treatment of blood diseases and artificial stem cell-made blood.
质粒dNA的提取、纯化与鉴定
本课程是由南方医科大学基因工程研究所生物化学与分子生物学实验教学中心提供的，内容主要讲述了质粒dNA的提取、纯化与鉴定的实验原理及实验材料方法和实验步骤
GE公司His标签蛋白纯化预装柱HisTrap™ FF Crude使用技巧
主要介绍了His标签蛋白纯化预装柱HisTrap™ FF Crude的实验过程，原理，说明，一般事项，样品制备，纯化操作， 按比例放大， 柱的清洗及保存等。
SIMdOL健康云服务及可穿戴医疗设备
自主知识产权、技术领先、专业门槛；创新的医疗健康管理模式：团队二三十年行业经验，稀缺资源；开放的服务平台；适用亿万级用户市场规模；持续增值的专业管理方法（模型）。中国至少有3~5亿适用人群，若以20%的市场购买率作为目标市场预测，将会有上亿规模的客户市场。本项目保守预测增速在60%以上，达产后的营收可达数亿元。
4d心电＆4G手机移动医疗（4d＆4G MI）
四维心电技术目前有两种产品形式：四维心电图仪（专用诊断仪器）和4d＆4G手机医疗（大众专业版）。两种产品利润可达上千万亿元（不含国际市场）.