Western Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel System(Aubin Penna.Ph.D)
Western Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel System(Aubin Penna.Ph.D)
Immunoblot Analysis Sean Gallagher(UVP,LLC)and Deb Chakravart(Proteomic Center)
Immunoblot Analysis Sean Gallagher(UVP,LLC)and Deb Chakravart(Proteomic Center)
病毒结构的一般原则 - Stephen Harrison P1
本视频由科普中国和生物医学大讲堂出品
Stephen Harrison (Harvard) Part 1: Virus structures: General principles
Harrison begins his talk by asking why most non-enveloped viruses and some enveloped viruses are symmetrical in shape. He proceeds to show us lovely images of the structures obtained by x-ray crystallography of numerous viral coat proteins. Deciphering these structures allowed scientists to understand that viral coat proteins form multimers, such as dimers and pentamers, which in turn interact with a scaffold that ensures that the coat proteins are correctly placed. This arrangement results in symmetrically shaped viruses.
In Part 1, Harrison also explains that enveloped viruses infect cells by inducing the fusion of the viral and host cell membranes. He delves deeper into the molecular mechanism of membrane fusion driven by the hemagglutinin or HA protein of the influenza virus in Part 2 of his talk.
Non-enveloped viruses, on the other hand, must enter cells by a mechanism other than membrane fusion. This is the focus of Part 3. Using rotavirus as a model, Harrison and his colleagues have used a combination of Xray crystallography and electron cryomicroscopy to decipher how the spike protein on the viral surface changes its conformation and perforates the cell membrane allowing the virus to enter the cell.
病毒的膜融合 - Stephen Harrison P2
本视频由科普中国和生物医学大讲堂出品
Stephen Harrison (Harvard) Part 2: Viral membrane fusion
Harrison begins his talk by asking why most non-enveloped viruses and some enveloped viruses are symmetrical in shape. He proceeds to show us lovely images of the structures obtained by x-ray crystallography of numerous viral coat proteins. Deciphering these structures allowed scientists to understand that viral coat proteins form multimers, such as dimers and pentamers, which in turn interact with a scaffold that ensures that the coat proteins are correctly placed. This arrangement results in symmetrically shaped viruses.
In Part 1, Harrison also explains that enveloped viruses infect cells by inducing the fusion of the viral and host cell membranes. He delves deeper into the molecular mechanism of membrane fusion driven by the hemagglutinin or HA protein of the influenza virus in Part 2 of his talk.
Non-enveloped viruses, on the other hand, must enter cells by a mechanism other than membrane fusion. This is the focus of Part 3. Using rotavirus as a model, Harrison and his colleagues have used a combination of Xray crystallography and electron cryomicroscopy to decipher how the spike protein on the viral surface changes its conformation and perforates the cell membrane allowing the virus to enter the cell.
非包膜病毒如何侵入细胞 - Stephen Harrison P3
本视频由科普中国和生物医学大讲堂出品
Stephen Harrison (Harvard) Part 3: Non-enveloped virus entry
Harrison begins his talk by asking why most non-enveloped viruses and some enveloped viruses are symmetrical in shape. He proceeds to show us lovely images of the structures obtained by x-ray crystallography of numerous viral coat proteins. Deciphering these structures allowed scientists to understand that viral coat proteins form multimers, such as dimers and pentamers, which in turn interact with a scaffold that ensures that the coat proteins are correctly placed. This arrangement results in symmetrically shaped viruses.
In Part 1, Harrison also explains that enveloped viruses infect cells by inducing the fusion of the viral and host cell membranes. He delves deeper into the molecular mechanism of membrane fusion driven by the hemagglutinin or HA protein of the influenza virus in Part 2 of his talk.
Non-enveloped viruses, on the other hand, must enter cells by a mechanism other than membrane fusion. This is the focus of Part 3. Using rotavirus as a model, Harrison and his colleagues have used a combination of Xray crystallography and electron cryomicroscopy to decipher how the spike protein on the viral surface changes its conformation and perforates the cell membrane allowing the virus to enter the cell.
Controlling the Cell Cycle: Cdk Substrates - David O. Morgan
本视频由科普中国和生物医学大讲堂出品
David O. Morgan (UCSF) Part 2: Controlling the Cell Cycle: Cdk Substrates
Cyclin-dependent kinases (Cdks) are the central components of the control system that initiates the events of the cell cycle. In the second part of this lecture, I discuss my laboratory's efforts to address the problem of how the Cdks trigger cell-cycle events. I describe our methods for identifying the protein substrates of the Cdks, and I discuss how these studies have led to important clues about how Cdks find their correct targets in the cell and how phosphorylation of those targets governs their function.
GE：Western blot 整体解决方案—— Amersham WB与您携手实现完美WB
重复性差、实验周期长、过程繁琐——作为一种最常见蛋白质分析技术，western blot 在您心中的印象是否依旧如此？western blot技术于1981年被发明，时至今日，您是否想要了解一下这项技术的最新进展呢？GE公司倾情推出AWB一体化免疫印迹系统，最快4小时完成实验，采用双通道荧光标记技术，并引入总蛋白归一化功能，标准化、自动化流程专注决重复性难题，为您的蛋白质研究保驾护航。