Cell and chemical biology of mitosis
Cell and chemical biology of mitosis
纳米生物效应与安全性实验室在2004年首次发现内含Gd原子的金属富勒烯三明治纳米结构颗粒可以直接作为肿瘤的高效低毒化疗药物以来，已经从分子免疫、神经调控、干细胞分化、血管生成等诸多方面对纳米颗粒直接作为高效低毒化疗药物的药效和机制，进行了长达6年多的研究，在国际学术刊物上连续发表了一系列的研究成果，逐渐形成较大的国际影响力。
simultaneous quantification of 47 gene expression in FFPE samples by a novel PCR-free approach
基因表达(gene expression)是指细胞在生命过程中，把储存在DNA顺序中遗传信息经过转录和翻译，转变成具有生物活性的蛋白质分子。生物体内的各种功能蛋白质和酶都是同相应的结构基因编码的。差别基因表达(differential gene expression)指细胞分化过程中，奢侈基因按一定顺序表达，表达的基因数约占基因总数的5%～10%。
李于：siRT1 Regulation of Energy Metabolism: Attenuation of Hepatic Steatosis and Obesity
Fibroblast growth factor 21 (FGF21) is the hepatocyte-derived hormone that regulates fatty acid metabolism and has potential to treat obesity and diabetes. We recently indicate that hepatic overexpression of siRT1 in diabetic mice attenuates hepatic steatosis and insulin resistance. However, the in vivo long-term consequence of hepatic siRT1 ablation in liver physiology remains unknown.
We showed that hepatocyte-specific siRT1 knockout (siRT1 LKO) mice with the albumin Cre-loxP system exhibited a striking phenotype with greater propensity for obesity on a chow diet, characterized by increased whole body mass and fat mass, reduced energy expenditure, and unaltered food intake and physical activity. The obese phenotypes of siRT1 LKO mice were associated with reduced hepatic and circulating levels of fasting FGF21.
Hepatic impairment of FGF21 repressed expression of key enzymes involving fatty acid oxidation such as CPT1α and MCAD, and inhibited expression of ketogenic enzymes including ACAT1, HMGCS2, HMGCL, and BDH1, thereby reducing plasma β–hydroxybutyrate levels in siRT1 LKO mice. Moreover, transcriptional activity of a FGF21 promoter-driven luciferase reporter was stimulated by siRT1 activators, resveratrol and SRT1720, in siRT1+/+ MEFs, but not in siRT1-/- MEFs.
The ability of resveratrol and SRT1720 to stimulate FGF21 protein was abolished by siRT1 H335A inactive mutant or by nicotinamide and splitomicin in HepG2 cells. Induction of FGF21 by siRT1 activators enhanced expression of key enzymes for fatty acid oxidation and ketogenesis.
These in vivo and in vitro findings characterize 1) hepatic siRT1 as a master regulator of FGF21; 2) siRT1-dependent activation of FGF21 in liver as a component for adaptive fasting response; and 3) defective hepatic siRT1 and FGF21 signaling as a key pathological determinant of energy metabolic abnormality and obesity susceptibility.
Generating B-lymphoblastoid cell lines using Epstein Barr virus transformation.
Generating immortalized B-lymphoblastoid cell lines via Epstein Barr virus transformation using the B95-8 EBV-infected and producing marmoset cell line.
Western Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel System(Aubin Penna.Ph.D)
Western Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel System(Aubin Penna.Ph.D)
Immunoblot Analysis Sean Gallagher(UVP,LLC)and Deb Chakravart(Proteomic Center)
Immunoblot Analysis Sean Gallagher(UVP,LLC)and Deb Chakravart(Proteomic Center)
枯草芽孢杆菌中芽孢的形成 - Richard Losick P1
本视频由科普中国和生物医学大讲堂出品
Richard Losick (Harvard) Part 1: Spore Formation in Bacillus Subtilis
How do simple cells differentiate, assemble into communities, and cope with change? Losick's seminar addresses these questions in the spore-forming bacterium Bacillus subtilis. Part I is an overview of how B. subtilis makes a spore.
多细胞生物的新研究 - Richard Losick P2
本视频由科普中国和生物医学大讲堂出品
Richard Losick (Harvard) Part 2: New Research on Multicellularity
Part II presents research on the capacity of B. subtilis cells to form architecturally complex communities.
枯草芽孢杆菌的不确定性和细胞结局 - Richard Losick P3
本视频由科普中国和生物医学大讲堂出品
Richard Losick (Harvard) Part 3: Stochasticity and Cell Fate
Part III presents research showing that B. subtilis uses a bet hedging strategy for coping with uncertainty.