LncrnA研究综合解决方案
lncrnA研究是目前rnA研究中最活跃的方向之一。在本视频中，俞伟平博士介绍了lncrnA的发现、起源、特点、分类以及有关的数据库，并结合研究案例介绍了lncrnA的功能以及lncrnA相关研究的活跃领域。俞博士还结合上海伯豪lncrnA相关产品及服务，全面系统地介绍了通过基因芯片和新一代测序等高通量手段对lncrnA进行发现、分析、验证和功能探索的方法。
Western Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel System(Aubin Penna.Ph.D)
Western Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel System(Aubin Penna.Ph.D)
端粒和端粒酶的作用 - Elizabeth Blackburn P1
本视频由科普中国和生物医学大讲堂出品
Elizabeth Blackburn (UCSF) Part 1: The Roles of Telomeres and Telomerase
Lecture Overview
Telomerase, a specialized ribonucleprotein reverse transcriptase, is important for long-term eukaryotic cell proliferation and genomic stability, because it replenishes the DNA at telomeres. Thus depending on cell type telomerase partially or completely (depending on cell type) counteracts the progressive shortening of telomeres that otherwise occurs. Telomerase is highly active in many human malignancies, and a potential target for anti-cancer approaches. Furthermore, recent collaborative studies have shown the relationship between accelerated telomere shortening and life stress and that low telomerase levels are associated with six prominent risk factors for cardiovascular disease.
端粒和端粒酶在人类干细胞和癌症中的作用 - Elizabeth Blackburn P2
本视频由科普中国和生物医学大讲堂出品
Elizabeth Blackburn (UCSF) Part 2: Telomeres and Telomerase in Human Stem Cells and in Cancer
Telomerase, a specialized ribonucleprotein reverse transcriptase, is important for long-term eukaryotic cell proliferation and genomic stability, because it replenishes the DNA at telomeres. Thus depending on cell type telomerase partially or completely (depending on cell type) counteracts the progressive shortening of telomeres that otherwise occurs. Telomerase is highly active in many human malignancies, and a potential target for anti-cancer approaches. Furthermore, recent collaborative studies have shown the relationship between accelerated telomere shortening and life stress and that low telomerase levels are associated with six prominent risk factors for cardiovascular disease.
基因分裂和rnA剪接 - Melissa Moore P1
本视频由科普中国和生物医学大讲堂出品
Melissa Moore (U. Mass/HHMI) Part 1: Split Genes and rnA Splicing
In the first part of her talk, Dr. Moore explains that eukaryotic pre-mrnA contains long stretches of non-protein coding sequences interspersed with protein coding regions. By recognizing specific sequences, cellular machinery splices out the non-coding introns leaving just the protein-coding exons in mrnA. Although at first glance this may seem like a wasteful process, it is splicing that facilitates the evolution of new genes, and alternative splicing that allows a limited number of genes to produce a large number of proteins.
线虫rnA干扰的简介
rnA干扰是一种应用广泛，将外源双链rnA引入到生物体内从而导致目的基因的表达下调的技术。在线虫中应用rnA干扰技术极为简便高 效，因仅需用表达与目的基因互补双链rnA的大肠杆菌喂食线虫即可实现。
首先，本短片将介绍rnA干扰的概念和解释它如何导致目的基因表达下调。然后我们 将演示在线虫中应用rnA干扰的步骤。包括大肠杆菌和rnAi线虫平板的制备，线虫培养以及如何评估线虫中rnA干扰的效果。rnA干扰经常用于反向遗传 学筛选以揭示哪些基因对于执行特定的生物过程至关重要。另外，自动化反向遗传筛选能使表达下调更高效和并可用于大规模基因分析。最后，rnA干扰经常用于 研究线虫的发育生物学。自它发明以来，科学家利用rnA干扰技术在解释许多生物学现象上取得重大进展。
【视频教程】揭开exornA的神秘面纱
rnA曾被认为只存在于细胞之中，在转录与翻译中起桥梁作用，但最新的研究发现，胞外囊泡外泌体中的rnA（exornA）可以经胞外囊泡外泌体转运到其他细胞中并发挥调节作用。让我们一起来透过视频介绍，揭开exornA的神秘面纱吧！
Unlocking the Mysteries of Extracellular rnA Communication
Once thought to exist only inside cells, rnA is now known to travel outside of cells and play a role in newly discovered mechanisms of cell-to-cell communication. Are these extracelluar rnAs (exrnAs) involved in diseases like cancer, heart disease or neurological diseases like Alzheimer's? Could scientists learn how to use exrnAs to diagnose diseases earlier or even to treat these diseases? The NIH Common Fund's Extracellular rnA Communication program aims to unlock the mysteries of exrnAs, uncovering their roles in human health and disease.