牛痘病毒如何进入细胞 - Ari HeleNIus P3
本视频由科普中国和生物医学大讲堂出品
Ari HeleNIus (ETH Zurich) Part 3: Open Sesame: Cell Entry and VacciNIa Virus
Part 3 focuses on a single virus, the VacciNIa virus, as a model for cell binding, signaling and endocytosis. Fluorescently labeled VacciNIa viruses bind to and surf along host cell filopodia. HeleNIus lab members noticed that when VacciNIa, unlike other viruses, reached the surface of the cell body it caused the plasma membrane to form blebs. Further experiments showed that the virus tricks the cell into thinking it is apoptotic debris. This induces blebbing and subsequent uptake of the virus by macropinocytosis. Additionally, automated high throughput siRNA screeNIng was used to screen a large number of infected cells for host genes required for VacciNIa virus uptake. Analysis of the genes identified allowed host factors and processes critical to viral infection to be identified. Expansion of this techNIque may provide a new source of information on pathogen-host interactions.
病毒包膜的内吞和渗透作用 - Ari HeleNIus P2
本视频由科普中国和生物医学大讲堂出品
Ari HeleNIus (ETH Zurich) Part 2: Endocytosis and Penetration
In the second lecture, the next steps in viral infection are described. Endocytosis of plasma membrane bound viruses can occur via a number of mechaNIsms including caveolar, clathrin, non-clathrin, or lipid raft mediated pathways. The internalized virus is enclosed in an endosome that may undergo increasing acidification resulting in acid mediated fusion between the viral envelope and the vesicle membrane. Following membrane penetration, the virus, once again, makes use of cellular machinery such as microtubules and their motors, to transfer its genome to the nucleus. HeleNIus describes experiments from his lab and others that have deciphered these complex processes.
布鲁斯·艾伯茨：LearNIng from Failure
Alberts declares "Success doesn't really teach you much, failure teaches you a lot." Speaking from his personal experience, Alberts asserts that all scientists make mistakes and suffer setbacks but learNIng from those failures is what allows one ultimately to succeed.
Pre-CliNIcal In Vivo Imaging Solutions from PerkinElmer
PerkinElmer offers a comprehensive portfolio of pre-cliNIcal in vivo imaging systems and reagents. Find out more at http://bit.ly/1mnyvwF. Our pre-cliNIcal imaging instruments include our highly published (over 4000 citations) IVIS Optical imaging systems, Quantum FX microCT which delivers high quality images at an x-ray dose low enough for longitudinal studies, and x-ray systems. We also offer a large portfolio of in vivo imaging reagents for most areas of research including cancer, infectious disease, stem cell, inflammation, toxicology/drug safety and more.
Introducing the Opera PheNIx™ High Content ScreeNIng System
Introducing Opera PheNIx™, a next-generation, confocal, high content screeNIng system designed for high-throughput, phenotypic screeNIng and assays using complex disease models, such as primary cells and microtissues. Find out more at http://bit.ly/1eZM4Ok.
Together we can help RevolutioNIzing Medicine
Together we can help RevolutioNIzing Medicine
罗氏NImbleGen序列捕获技术在农业研究中的应用
近几年来，高通量测序技术在现代农业研究中得到了越来越多的应用，为新品种选育和品质改良带来了新的科研方法和解决方案。然而，研究者想要对农作物基因组进行深入分析依然面临诸多挑战。一方面，植物的基因组比较复杂，往往都是多倍体；另一方面，我们目前掌握的大多数植物的基因组信息并不完整，这都给我们的前期实验和后续分析带来了各种问题。庞大的植物基因组包含的大多数信息都属于重复序列，有用的信息往往位于编码区域。采用传统的测序方法对植物全基因组测序带来的大量信息既增加了测序成本，也加大了后续数据分析的难度。罗氏NImbleGen针对农业相关研究推出了一系列的靶向富集产品，可以帮助研究者把测序的目标精确定位在植物的外显子区域或者其他任意感兴趣区段。
NImbleGen特有的探针设计算法和超高密度探针合成技术，将使您对任意植物基因组的靶向富集达到前所未有的精度，大大节省后续的测序和分析成本。本次讲座将围绕罗氏NImbleGen针对农业相关研究推出的序列捕获产品进行应用介绍，并为大家详细分析各种实际应用案例，希望能为您的研究提供一个全新的思路。
PureBlu™ Hoechst 33342 Nuclear StaiNIng Dye for Live Cells - A Fast Approach to StaiNIng Nuclei
This brief tutorial demonstrates the use of the PureBlu Hoechst 33342 Dye with the ZOE™ Fluorescent Cell Imager for routine nuclear staiNIng in fluorescence microscopy and cell imaging applications.
Where are proteins folded by chaperoNIns?
见习了伴侣辅助蛋白质折叠那蛋白质折叠的分子伴侣在哪里？ 在机器内部和外部的解决方案？在这个讲座中Art Horwich分享道：确定顺式拓扑的加成的关键顺序 ，从格罗尔的生产力的释放，从格罗尔的生产力的释放，一种单环三维结构的版本4的同步替换建设同时代入使用一个GroES陷阱识别生产多肽释放网站，生产折叠格罗尔发生在一个GroES封装顺室。
Ronald Germain（NIAID/NIH）：美国国立卫生研究院的资助应该支持的人，没有项目
Ronald Germain链接许多与美国国立卫生研究院资助的生物医学研究的当前问题，如增加资金竞争，拒绝科学的职业生涯中的有才华的学生，和抑制创造力，到R01资助制度。他提出了一个系统，取代R01资助支持个人的系统，没有项目，并持有通过回顾性修订负责人。他的建议是由美国国立卫生研究院在成功资助计划的影响NIH校内程序和HHMI。有关这项建议的更多细节，读他的评论“愈合的NIH资助的生物医学研究企业”2015年6月18日在细胞中发表。