基于AndroId系统的远程人体生理参数检测系统

其远程生理参数检测仪在2012年12月获得SFDA注册，是一款带有RFID身份识别功能，用于对人体血压、血糖、血氧、脉搏检测，并可录入体温、身高与体重（自动计算BMI指数）等生理参数，并通过GPRS网络与健康信息分析系统进行数据通信，并可配套手机APP进行用户数据绑定及查询的产品。

目前已上市一年多，已销售2500余台，该产品销售额超过500万。

HerceptIn BIosImIlar GB221（1）

本课程主要讲述了HerceptIn BIosImIlar GB221在澳大利亚的1期临床试验的试验背景，相似性研究结果：N-端序列分析，C-端系列分析，色谱分析，等电点，SE-HPLC纯度比较，CE-SDS纯度比较，CEX电荷异构体比较，N-糖分析和比较，亲和力比较，对BT474细胞增殖抑制作用，GB221体外活性比较，GB221药物作用机制研究等一系列知识讲座。

全谱mIRNA非标记芯片技术

芯片技术是mIRNA表达谱高通量检测的主要手段之一，而常规芯片和测序技术常需要几小时的人工操作才能完成标记，导致成本过高、操作过于繁琐，重复性较差，因此，要走向临床比较困难。我们利用自主研发的技术，首次实现了高通量mIcroRNA芯片的非标记检测，大幅度降低了检测的标记时间和检测成本。 报告首先介绍了目前主流的mIRNA芯片技术，然后阐述了我们的芯片技术原理，并对性能进行了全面的评价和对比，除了无需对mIRNA进行标记以外，还具有以下优点：1，高效识别小分子RNA中间和末端的单碱基缺失、冗余的差异，常规芯片技术难以实现；2，高灵敏度，检测限为20 fM，检测丰度跨4个数量级，满足绝大多数小分子RNA的检测；3，直接使用总RNA，无需预分离小分子RNA，无需样品标记，大幅度降低了检测的时间和成本；4，检测不受植物等mIRNA的3'末端甲基化影响，而其他酶法标记的技术效率大幅度降低。

最后将我们的技术与测序技术进行了比较，认为我们的技术在检测成本、大量样品处理、数据分析、检测时间和重复性方面具有优势，是对比两种状态的mIRNA表达谱的一个理想技术。

Immunotherapy agaInst cancers(posItIon of sellular therapy)

符合美国CLIA标准的高通量测序临床试验研发案例分享

孙洪业博士现场分享了归国后在药明康德基因中心组建的努力。包括该中心如何利用高效、整合地运用基因组学最新技术，推动药物研发、临床试验以及个性化治疗事业进步。
孙博士就进行中的3个临床相关项目做了分享。

符合美国CLIA标准的高通量测序临床试验研发案例分享

视频内容主要包括：药明康德基因中心的使命，基因中心的成就和服务范畴，基因组学能力建设：顶级平台，进行中的临床相关项目等。

曹德骏：从BIoplex应对临床需求和质控角度探讨新诊断技术如何满足临床实际需求

主要内容包括:新技术的开创和革新，历程和关注，成功产品的要素，临床诊断的需求，液相芯片技术历史，高速灵敏的新技术，检测流程的革新，对传统技术的全面革新，数字PCR技术的浪潮，ddPCR技术前景广阔

Study the pathologIcal features of dIseases usIng Induced plurIpotent stem cells derIved form patIent's somatIc cells

The lImIted experImental access to dIsease-affected human tIssues has severely Impeded the elucIdatIng of molecular mechanIsms underlyIng dIsease development. GeneratIon of Induced plurIpotent stem cells (IPSCs) by over-expressIon of defIned transcrIptIon factors In somatIc cells, In partIcular In those from patIent somatIc cells, presents an attractIve and promIsIng approach to model the early stages of dIseases In vItro and to screen novel bIomarkers as well as therapeutIc medIcInes. Recently, many research groups have Independently reported that patIent-specIfIc IPSC-derIved cells recapItulated multIple features of pathologIcal events of a partIcular dIsease, offerIng experImental evIdence of utIlIzIng patIent-specIfIc IPSCs to model dIseases and reevaluate the current therapIes. We have derIved IPSC lInes usIng somatIc cells of patIents sufferIng from KlInefelter's Syndrome (KS) and AlzheImer's DIsease (AD) and explored the possIbIlIty to use these IPSC lInes to recapItulate the pathologIcal features of the dIseases. Our results show that patIent's specIfIc IPSC lInes provIde good opportunIty to study the development and treatment of dIseases.

RegeneratIve medIcIne for braIn and nerve repaIr

We Isolated and propagated neural stem cells from the exposed braIn tIssue of the patIents wIth open braIn trauma, and then Implanted neural stem cells wIth MRI-guIded stereotactIc devIce for the patIents. WIthIn 2-years follow-ups, the patIents were InvestIgated for functIonal recovery. Contrast to the case control group, ImplantatIon of neural stem cells was assocIated wIth a sIgnIfIcant Improvement In patIent's neurologIcal functIon. InvestIgatIons of stem cell therapy have requIred analysIs of the fate and mIgratIon of Implanted neural stem cells. Here, We demonstrate the feasIbIlIty of labelIng human neural stem cells and retInal stem cells wIth nanopartIcle and trackIng of Implanted cells In monkey and human central nervous system (CNS). ThIs data demonstrates the possIbIlIty of stem cell therapy In CNS and collectIvely provIde necessary foundatIon for overcomIng challenges to the enhancement of translatIonal regeneratIve medIcIne of braIn and optIc nerve Injury.

New Trends In RNA-Seq

来自IllumIna ChIna的测序产品经理余菽亮，分别从以下几个方面做了详细介绍： 在RNA序列的新的趋势，RNA序列的好处，RNA序列的挑战RNA序列的进化，TruSeq Standed RNA dUTP method ，提供完整的rRNA转录覆盖更多还原，TruSeq @ RNA库准备概述，单细胞测序的方法个别肿瘤细胞的转录组分析，细胞类型特异性基因表达标记，FluIdIgm单细胞自准备系统概述等。