ReGEnerative medicine for brain and nerve repair
We isolated and propagated neural stem cells from the exposed brain tissue of the patients with open brain trauma, and then implanted neural stem cells with MRI-guided stereotactic device for the patients. Within 2-years follow-ups, the patients were investigated for functional recovery. Contrast to the case control group, implantation of neural stem cells was associated with a significant improvement in patient's neurological function. Investigations of stem cell therapy have required analysis of the fate and migration of implanted neural stem cells. Here, We demonstrate the feasibility of labeling human neural stem cells and retinal stem cells with nanoparticle and tracking of implanted cells in monkey and human central nervous system (CNS). This data demonstrates the possibility of stem cell therapy in CNS and collectively provide necessary foundation for overcoming challenGEs to the enhancement of translational reGEnerative medicine of brain and optic nerve injury.
sRNA Induces the LarGE-scale Transdetermination of Mesenchymal Stem Cells into Hematopoietic Stem Cells in Human.
Mesenchymal stem cells (MSCs) can differentiate into cells of bone, endothelium, adipose tissue, cartilaGE, muscle, and brain. However, whether they can transdeterminate into hematopoietic stem cells (HSCs) remains unsolved. We report here that a subpopulation of human MSCs that are CD44+,CD29+, CD105+, CD166+,CD133-,CD34- could differentiate into hematopoietic stem cells (CD150+/CD133+/CD34+) and their descending blood cells in vitro, when transfected with new endoGEnous shRNAs The sRNA was high-effectively delivered into MSCs by a novel peptide means. These induced MSC-HSCs could form different types of hematopoietic colonies as nature-occurring HSCs did. Upon transplantation into sublethally irradiated NOD/SCID mice, these MSC-HSCs engrafted and differentiated into all hematopoietic lineaGEs such as erythrocytes, lymphocytes, myelocytes and thrombocyte. More importantly, these induced HSCs could successfully engraft and effectively function in patients with severe aplastic anemia. Furthermore, we demonstrated the first evidence that the transdetermination of MSCs was induced by acetylation of histone proteins and activation of many transcriptional factors. ToGEther, our findings identify the sRNAs that dictates a directed differentiation of MSCs toward HSCs and open up a new source for HSCs used for the treatment of blood diseases and artificial stem cell-made blood.
GE公司His标签蛋白纯化预装柱HisTrap™ FF Crude使用技巧
主要介绍了His标签蛋白纯化预装柱HisTrap™ FF Crude的实验过程，原理，说明，一般事项，样品制备，纯化操作， 按比例放大， 柱的清洗及保存等。
ReGEnerative medicine for brain and nerve repair
We isolated and propagated neural stem cells from the exposed brain tissue of the patients with open brain trauma, and then implanted neural stem cells with MRI-guided stereotactic device for the patients. Within 2-years follow-ups, the patients were investigated for functional recovery. Contrast to the case control group, implantation of neural stem cells was associated with a significant improvement in patient's neurological function. Investigations of stem cell therapy have required analysis of the fate and migration of implanted neural stem cells. Here, We demonstrate the feasibility of labeling human neural stem cells and retinal stem cells with nanoparticle and tracking of implanted cells in monkey and human central nervous system (CNS). This data demonstrates the possibility of stem cell therapy in CNS and collectively provide necessary foundation for overcoming challenGEs to the enhancement of translational reGEnerative medicine of brain and optic nerve injury.
ImaGE J 快速入门及基本操作
ImaGE J是一个基于java的公共图像处理软件。
1、支持 TIFF, PNG, GIF, JPEG, BMP, DICOM, FITS 等多种格式
2、可打开任意多图像
3、缩放，旋转，扭曲，平滑
4、图片的区域和像素统计，间距，角度计算，创建柱状图和剖面图，傅里叶变换
ImaGE J 应用实例
来自生物谷的孔静同学为大家带来“ImaGE J 应用”，内容包括：
一，多色荧光图片叠加；
二，免疫组化及荧光强度分析；
三，连续照片合成视频
TarGEts-based therapy for leukemia: opportunity and challenGE
优点主要为：能增强患者的免疫力，防止肿瘤的转移和复发，对病人机体的损伤小。 在我国，现在普遍开展的树突状细胞（DC）和细胞因子诱导的杀伤细胞（CIK）的生物疗法被广泛应用。
GEnomics in the “Century of Biology”
完整基因组测序已为包括肺癌在内的若干种癌症类型的突变谱提供了线索。最新测序技术意味着，现在有可能从全基因组范围内来观察突变差异，而且现在研究人员对肺癌已经做到了这一点