RiboZERo和方向RNA库-陈巍学基因（15）
本节课程主要内容：
1.RiboZERo与Poly（T）方法对比的技术优势
2.RiboZERo的技术原理
3.建定向的RNA库的方法：
1）掺入U碱基的方法来标识cDNA的第二条链
2）ScriptSeq方法
金颖：Fox3 suppresses NFAT-mediated diffERentiation to maintain self-renewal of embryonic stem cells
金颖教授为分子发育生物学研究室主任，健康科学中心研究员。金教授介绍了Fox3通过抑制NFAT介导的分化维持了胚胎干细胞的自我更新的机制等前沿发现。
Pluripotency-associated transcription factor Foxd3 is required for maintaining pluripotent cells. HowevER, molecular mechanisms undERlying its function are largely unknown.
HERe, we report that Foxd3 suppresses diffERentiation induced by Calcineurin-NFAT signaling to maintain the ESC identity. Mechanistically, Foxd3 intERacts with NFAT proteins and recruits co-repressor Tle4, a membER of the Tle suppressor family highly expressed in undiffERentiated ESCs, to repress NFATc3’s transcriptional activities.
FurthERmore, global transcriptome analysis shows that Foxd3 and NFATc3 co-regulate a set of diffERentiation-associated genes in ESCs. Collectively, our study establishes a molecular and functional link between a pluripotency-associated factor and an important ESC diffERentiation-inducing pathway.
GenERating B-lymphoblastoid cell lines using Epstein Barr virus transformation.
GenERating immortalized B-lymphoblastoid cell lines via Epstein Barr virus transformation using the B95-8 EBV-infected and producing marmoset cell line.
WestERn Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel System(Aubin Penna.Ph.D)
WestERn Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel System(Aubin Penna.Ph.D)
Immunoblot Analysis Sean GallaghER(UVP,LLC)and Deb Chakravart(Proteomic CentER)
Immunoblot Analysis Sean GallaghER(UVP,LLC)and Deb Chakravart(Proteomic CentER)
细菌交流通过群感效应 - Bonnie BasslER P1
本视频由科普中国和生物医学大讲堂出品
Bonnie BasslER (Princeton) Part 1: BactERial Communication via Quorum Sensing
BactERia, primitive single-celled organisms, communicate with chemical languages that allow them to synchronize their behavior and thEReby act as enormous multi-cellular organisms. This process is called quorum sensing and it enables bactERia to successfully infect and cause disease in plants, animals, and humans. Investigations of the molecular mechanisms undERlying quorum sensing are leading to the development of novel strategies to intERfERe with quorum sensing. These strategies form the basis of new thERapies to be used as antibiotics. See more at http://www.ibioseminars.org
霍乱弧菌群体感应和新型抗生素 - Bonnie BasslER P2
本视频由科普中国和生物医学大讲堂出品
Bonnie BasslER (Princeton) Part 2: Vibrio CholERae Quorum Sensing and Novel Antibiotics
BactERia, primitive single-celled organisms, communicate with chemical languages that allow them to synchronize their behavior and thEReby act as enormous multi-cellular organisms. This process is called quorum sensing and it enables bactERia to successfully infect and cause disease in plants, animals, and humans. Investigations of the molecular mechanisms undERlying quorum sensing are leading to the development of novel strategies to intERfERe with quorum sensing. These strategies form the basis of new thERapies to be used as antibiotics. See more at http://www.ibioseminars.org