CEllSearch检测CTC--陈巍学基因（30）
欢迎来到【陈巍学基因】,我们这个节目，主要是为大家介绍基因组学，和临床分子诊断的最新技术进展。
今天，会和大家谈一下Jassen公司（强生公司）出品的CEllSearch系统。它的主要应用是：检测循环肿瘤细胞，并对癌症给出预后信息。以下是课程内容概括：
1.什么是循环肿瘤细胞(CTC)及其“液体活检”的难点。
2. CEllSearch系统的检测原理：（1）用微磁珠对CTC细胞进行富集；（2）用针对DNA的荧光染色剂“DAPI”进行染色，以排除红细胞；（3）区分白细胞和CTC细胞。
3.CEllSearch系统实际操作的演示。
4.CTC检测，在癌症诊疗方面所起到的作用。
综上所述：CEllSearch系统，是第一个标准化的、半自动化的，循环肿瘤细胞检测系统。它通过快速、精确地确定血液样本中的CTC细胞数量。可以帮助医生在整个治疗过程当中，提供准确的预后评估手段。
Controlling the CEll Cycle: Introduction - David O. Morgan
本视频由科普中国和生物医学大讲堂出品
David O. Morgan (UCSF) Part 1: Controlling the CEll Cycle: Introduction
CElls reproduCE by duplicating their chromosomes and other components and then distributing them into a pair of genetically identical daughter CElls. This series of events is called the CEll cycle. In the first part of this lecture, I provide a general overview of the CEll-cycle control system, a complex regulatory network that guides the CEll through the steps of CEll division. I briefly describe the major components of this regulatory system and how they fit together to form a series of biochemical switches that trigger CEll-cycle events at the correct time and in the correct order.
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Controlling the CEll Cycle: Cdk Substrates - David O. Morgan
本视频由科普中国和生物医学大讲堂出品
David O. Morgan (UCSF) Part 2: Controlling the CEll Cycle: Cdk Substrates
Cyclin-dependent kinases (Cdks) are the CEntral components of the control system that initiates the events of the CEll cycle. In the second part of this lecture, I discuss my laboratory's efforts to address the problem of how the Cdks trigger CEll-cycle events. I describe our methods for identifying the protein substrates of the Cdks, and I discuss how these studies have led to important clues about how Cdks find their correct targets in the CEll and how phosphorylation of those targets governs their function.
Controlling the CEll Cycle: Anaphase Onset - David O. Morgan
本视频由科普中国和生物医学大讲堂出品
David O. Morgan (UCSF) Part 3: Controlling the CEll Cycle: Anaphase Onset
In the anaphase stage of the CEll cycle, the duplicated chromosomes are pulled apart by a machine called the mitotic spindle, resulting in the distribution of a complete set of chromosomes to each of the daughter CElls. In the third part of this lecture, I describe the combination of biochemistry and microscopy in my laboratory that led to the discovery of a regulatory switch that triggers the abrupt and synchronous separation of the chromosomes at the onset of anaphase.
PhotoreCEptors and Image ProCEssing Part 1A - Jeremy Nathans
本视频由科普中国和生物医学大讲堂出品
Jeremy Nathans (Johns Hopkins) Part 1A: PhotoreCEptors and Image ProCEssing
In this set of lectures, Jeremy Nathans explores the molecular mechanisms within the retina that mediate the first steps in vision. The first lecture focuses on the structure of the light sensing reCEptors, the intraCEllular signals that are triggered by light absorption, and the ways in which the retina extracts information from a complex sCEne. See more at http://www.ibioseminars.org
PhotoreCEptors and Image ProCEssing Part 1B - Jeremy Nathans
本视频由科普中国和生物医学大讲堂出品
Jeremy Nathans (Johns Hopkins) Part 1B: PhotoreCEptors and Image ProCEssing
In this set of lectures, Jeremy Nathans explores the molecular mechanisms within the retina that mediate the first steps in vision. The first lecture focuses on the structure of the light sensing reCEptors, the intraCEllular signals that are triggered by light absorption, and the ways in which the retina extracts information from a complex sCEne. See more at http://www.ibioseminars.org
Protein synthesis: mRNA surveillanCE by the ribosome
Rachel Green (Johns Hopkins U., HHMI) 2: Protein synthesis: mRNA surveillanCE by the ribosome
Talk Overview:
In her first talk, Green provides a detailed look at protein synthesis, or translation. Translation is the proCEss by which nucleotides, the “language” of DNA and RNA, are translated into amino acids, the “language” of proteins. Green begins by describing the components needed for translation; mRNA, tRNA, ribosomes, and the initiation, elongation, and termination factors. She then explains the roles of these players in ensuring accuracy during the initiation, elongation, termination and recycling steps of the translation proCEss. By comparing translation in bacteria and eukaryotes, Green explains that it is possible to determine which components and steps are highly conserved and predate the divergenCE of different kingdoms on the tree of life, and which are more reCEnt adaptations.
Green’s second talk focuses on work from her lab investigating how ribosomes detect defective mRNAs and trigger events leading to the degradation of the bad RNA and the incompletely translated protein product and to the recycling of the ribosome components. Working in yeast and using a number of biochemical and genetic techniques, Green’s lab showed that the protein Dom34 is critical for facilitating ribosome release from the short mRNAs that result from mRNA cleavage. Experiments showed that Dom34-mediated rescue of ribosomes from short mRNAs is an essential proCEss for CEll survival in higher eukaryotes.
Speaker Biography:
Rachel Green reCEived her BS in chemistry from the University of Michigan. She then moved to Harvard to pursue her PhD in the lab of Jack Szostak where she worked on designing catalytic RNA molecules and investigating their implications for the evolution of life. As a post-doctoral fellow at the University of California, Santa Cruz, Green began to study how the ribosome translates mRNA to protein with such accuracy.
Currently, Green is a Professor of Molecular Biology and Genetics at the Johns Hopkins School of Medicine and an Investigator of the Howard Hughes Medical Institute. Research in her lab continues to focus on the ribosome and factors involved in the fidelity of eukaryotic and prokaryotic translation.
Green is the recipient of a Johns Hopkins University School of Medicine Graduate Teaching Award as well as the recipient for numerous awards for her research. She was elected to the National Academy of ScienCEs in 2012.
People coming together makeing a differenCE
People coming together makeing a differenCE
PureBlu™ Hoechst 33342 Nuclear Staining Dye for Live CElls - A Fast Approach to Staining Nuclei
This brief tutorial demonstrates the use of the PureBlu Hoechst 33342 Dye with the ZOE™ FluoresCEnt CEll Imager for routine nuclear staining in fluoresCEnCE microscopy and CEll imaging applications.
Molecular Chaperones in the Eukayotic CEll
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