Agilent生物芯片原理--陈巍学基因（29）
欢迎来到【陈巍学基因】，我们这个节目，主要是为大家介绍基因组学，和最新的临床分子诊断的技术进展。
今天，会和大家谈一下Agilent公司（安捷伦公司）的生物芯片，视频主要分为以下几个部分：
1.Agilent生物芯片的扫描仪、合成工艺、大体规格和分析软件等。
2.Agilent芯片的应用主要在“比较基因组杂交”领域；
CGH芯片主要是检测：杂合性缺失（LOH）、单亲二染色体（UPD）、和拷贝数变异（CNV）；
CGH芯片区分SNP位点的方法，是通过“酶切+杂交”。
3.Agilent表达谱芯片的IVT检测原理和三个特点。
美国大学预修课程：生物实验室课程 - part 1
Paul Andersen详细介绍了美国大学预修课程的13个生物学实验。本课题的研究内容包括：人工选择， 哈迪-温伯格平衡定律，用BLAST比对DNA， 扩散和渗透、光合作用、呼吸、有丝分裂和减数分裂的基因。
美国大学预修课程：生物实验室课程 - part 2
Paul Andersen详细介绍了美国大学预修课程的13个生物学实验。本课题的研究内容包括：人工选择， 哈迪-温伯格平衡定律，用BLAST比对DNA， 扩散和渗透、光合作用、呼吸、有丝分裂和减数分裂的基因。
Generating B-lymphoblastoid cell lines using Epstein Barr virus transformation.
Generating immortalized B-lymphoblastoid cell lines via Epstein Barr virus transformation using the B95-8 EBV-infected and producing marmoset cell line.
使用Nanodrop ND-1000的分光光度仪测定DNA
Spectrophotometric evaluation of DNA using a Nanodrop ND-1000. Spectrophotometric evaluation of genomic DNA quality and quantity using a microvolumetric Nanodrop ND-1000 optical fibre UV-Vis spectrophotometer.
使用ficoll-1077分离外周血单核细胞
Isolation of peripheral blood mononuclear cells using ficoll-1077. Isolation of human peripheral blood mononuclear cells (PBMC's) from whole blood using ficoll-1077 density gradient.
Western Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel System(Aubin Penna.Ph.D)
Western Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel System(Aubin Penna.Ph.D)
CellSearch检测CTC--陈巍学基因（30）
欢迎来到【陈巍学基因】,我们这个节目，主要是为大家介绍基因组学，和临床分子诊断的最新技术进展。
今天，会和大家谈一下Jassen公司（强生公司）出品的CellSearch系统。它的主要应用是：检测循环肿瘤细胞，并对癌症给出预后信息。以下是课程内容概括：
1.什么是循环肿瘤细胞(CTC)及其“液体活检”的难点。
2. CellSearch系统的检测原理：（1）用微磁珠对CTC细胞进行富集；（2）用针对DNA的荧光染色剂“DAPI”进行染色，以排除红细胞；（3）区分白细胞和CTC细胞。
3.CellSearch系统实际操作的演示。
4.CTC检测，在癌症诊疗方面所起到的作用。
综上所述：CellSearch系统，是第一个标准化的、半自动化的，循环肿瘤细胞检测系统。它通过快速、精确地确定血液样本中的CTC细胞数量。可以帮助医生在整个治疗过程当中，提供准确的预后评估手段。
克氏锥虫和Chagas病 - Norma Andrews P1
本视频由科普中国和生物医学大讲堂出品
Norma Andrews (U. Maryland) Part 1: Trypanosoma cruzi and Chagas’ Disease
Lecture overview:
Trypanosoma cruzi and Leishmania are closely related intracellular protozoan parasites that cause serious diseases throughout the world. In the first part of this lecture, I will present background material on the biology of Trypanosoma cruzi and the history of its discovery as an important agent of human disease in Latin America. I will also discuss the main characteristics of the disease, and the current efforts to stop human transmission.
In the second part of this lecture, I will present background material on Leishmania, the intracellular protozoan parasites responsible for severe human pathology in several parts of the world. I will discuss the main disease forms, the history of identification of the causative agent and form of transmission, and recent discoveries that established important concepts in our understanding of this increasingly serious infectious disease.
In the third part of this lecture, I will discuss current work from our laboratory on mechanisms used by the intracellular parasites Trypanosoma cruzi and Leishmania to interact with mammalian cells. In addition to clarifying specific molecular strategies used by these parasites to infect and survive within host cells, these studies also led, in some instances, to unexpected insights on novel pathways regulating mammalian cell function.
Speaker bio:
Norma Andrews is currently a Professor and Chair of the Department of Cell Biology and Molecular Genetics at the University of Maryland. She received a B.S. degree in biology (1977) and a Ph.D. degree in biochemistry (1983) from the University of São Paulo, Brazil.
In 1990, after completing postdoctoral studies in the laboratory of Victor Nussenzweig at New York University, she was appointed Assistant Professor at Yale University where she remained until 2010.
Andrews was a Burroughs Wellcome New Investigator, a Burroughs Wellcome Molecular Parasitology Scholar and recipient of a NIH MERIT Award. Her laboratory has made numerous contributions to the cell biology of host-pathogen interactions, and discoveries in this area have led to the identification and functional characterization of a novel pathway of Ca2+-regulated lysosomal exocytosis in mammalian cells.