杨春:体外诊断市场分析与展望
杨春,生物谷联合创始人,BioInsight 首席分析师。 杨总就一下几个方面做了演讲分享: 1.中国医药市场现状及市场驱动因素, 2.中国体外诊断市场现状及产业链 3.2009-2017全球IVD市场规模及预测 4.2009-2017中国IVD市场规模及预测 5.IVD未来发展趋势及各模式的优点及局限性
高峰:浓缩灌流培养方法在细胞培养工艺中的应用案例和应用中的初步成本分析
随着新技术的开发和应用,浓缩灌流培养技术(Concentrated Perfusion)在哺乳动物细胞培养生产中日渐成熟。作为国内第一家CMO公司,我们应用Refine Technology的ATF系统,对生物类似抗体药的工艺进行改造,应用一次性生物反应器和浓缩系统,开发了新的浓缩灌流工艺,细胞密度达到160X10E6/ml,抗体蛋白产量达到20g/L。
生物类似药物未来市场竞争激烈,而生产产量的提升意味着产品成本的降低。对于浓缩灌流培养技术对产品生产成本的影响,我们进行了初步的估算和分析。这项技术在抗体类药物大规模生产,尤其是生物类似药物大规模生产应用方面将具有广阔前景。
表达谱芯片的应用及数据分析方案
表达谱芯片是在基因的表达、调控、功能分析等基因学研究中应用最广泛的芯片。本视频先介绍了表达谱芯片的类型、探针设计及主要产品,随后介绍了芯片分析结果的验证方法。结合上海伯豪最新的服务案例,讲师详细说明了表达谱芯片在不同领域中的应用。最后视频还在表达谱芯片的数据分析及结果展示以及表达谱芯片用于ceRNA (competing endogenous RNA) 研究等方面提供了有价值的信息。
单分子分析方法研究运动蛋白
Molecular motor proteins are fascinating enzymes that power much of the movement performed by living organisms. In the first part of this lecture, I will provide an overview of the motors that move along cytoskeletal tracks (kinesin and dynein which move along microtubules and myosin which moves along actin). The main focus of this lecture is on how motor proteins work. How does a nanoscale protein convert energy from ATP hydrolysis into unidirectional motion and force production? What tools do we have at our disposal to study them? The first part of the lecture will focus on these questions for kinesin (a microtubule-based motor) and myosin (an actin-based motor), since they have been the subject of extensive studies and good models for their mechanisms have emerged. I conclude by discussing the importance of understanding motor proteins for human disease, in particular illustrating a recent biotechnology effort from Cytokinetics, Inc. to develop drugs that activate cardiac myosins to improve cardiac contractility in patients suffering from heart failure. The first part of the lecture is directed to a general audience or a beginning graduate class.
In the second part of this lecture, I will discuss our laboratories current work on the mechanism of movement by dynein, a motor protein about which we still know very little. This is a research story in progress, where some advances have been made. However, much remains to be done in order to understand how this motor works.
The third (last) part of the lecture is on mitosis, the process by which chromosomes are aligned and then segregated during cell division. I will describe our efforts to find new proteins that are important for mitosis through a high throughput RNAi screen. I will discuss how we technically executed the screen and then focus on new proteins that are we discovered that are involved in generating the microtubules that compose the mitotic spindle. I also discuss the medical importance of studying mitosis, including the development of drugs targeted to mitotic motor proteins, which are currently undergoing testing in clinical trials.
qPCR基因表达分析的完整实验流程
在基因表达研究中,real-time quantitative RT-PCR (qRT-PCR) 检测技术带给我们快速、灵敏、精细定量的检测体验。
然而您是否曾留意过:使用不同质量的样品,不同的反转录程序、检测方法(染料或探针)、qPCR平台、试剂及扩增程序等对实验结果造成的不同影响?您是否依然坚信qRT-PCR是基因表达定量的“金标准”?您如何理解不同实验室对某一生物学现象的相悖的研究结果呢?
在qPCR研究领域,已经有很多学者研讨了可能引起qPCR研究结果误差的影响因素,以及相应的质控方法。"MIQE"正是这样一个指导研究人员进行整个实验设计、操作、研究结果分析、报告的参考指南。6月11日,罗氏邀请您一起研习这份指南!
痕量分析,你用对溶剂了吗?
痕量分析的方法及应用; 高纯溶剂对于检测结果准确性的重要性; 杂质干扰检测结果的具体表现; Fisher Chemical 高纯溶剂针对痕量分析的解决方案;
荧光原位杂交和染色体核型分析
A video protocol and description of Fluorescence In Situ Hybridization (FISH) in plants. The following two companion resources contain recipes, probe plasmid data, and other information needed for doing the procedures illustrated in this video. Of further interest are suggestions for developing a karyotyping cocktail for other plant species and making probes from BAC DNA.
从实验到分析——深度解析基于二代测序的LncRNA研究及应用
非编码RNA起初被认为是基因组转录的“垃圾”,而近年来,多个高水平的杂志以封面形式报道长链非编码RNA(long non-coding RNA,LncRNA)的重要性,由此LncRNAs引起了人们广泛的关注,已成为各研究领域的一颗新星。
本次讲座主要将从以下几个方面展开:
①LncRNA的方案设计及研究思路
②LncRNA的实验及分析流程
③LncRNA的案例解析
④LncRNA+mRNA+miRNA联合分析方案解析
⑤一类特殊的ncRNA——circular RNA
电子实验记录本在配方及分析领域应用
用电子实验记录本来记录和管理实验室数据,已是企业确保研发知识积累,促进流程优化的大势所趋。 PerkinElmer 电子实验记录本(前身 Cambridge 电子实验记录本,在化学、生物及配方工艺领域有着非常出色的表现。本次 Webinar 会结合 PerkinElmer 目前已经完成的项目实施,向大家介绍电子实验记录本如何替代纸质记录本,帮助和简化配方部门的工作内容,管理繁复的仪器、药品、原辅料信息,实现部门和部门之间的简单工作流。
主要内容:
1.电子实验记录本如何应用于配方及工艺研究
2.利用电子实验记录本解决实际制剂所研究方案范例
3.如何用电子实验记录本管理内部、外部仪器及产品库存
4.利用电子实验记录本实现简单工作流管理