印度斑马鱼:斑马鱼的介绍
印度斑马鱼是一种小型淡水鱼类,用作生物医学研究中的模式生物。这种鱼类的优点包括它们和人类基因高度保守的相似,以及它们的维护简单、低廉。另外,基因表达在斑马鱼的胚胎中很容易控制,而且它们的透明体征允许我们观察它们的发育过程。
在介绍斑马鱼的优点之前,本视频将首先介绍斑马鱼的基本生物学,包括系统发育、生命周期以及自然环境。本视频还将概述斑马鱼的研究历史,将回顾其中的主要发现,包括从早期建立有效的遗传学筛选方法到为人类疾病发现新型的治疗方法,例如癌症。最后,还将讨论许多斑马鱼实验中的一些,包括免疫学和发育学方面的研究。
RNAscope®技术原理介绍
RNAscope®®原位定量专利技术由美国新兴的分子病理领导者ACD公司(Advanced Cell Diagnostics, Inc., California, USA)开发,通过专利的双“Z”探针设计,使RNA原位杂交具有高度特异性、单分子检测的敏感性并有极高的信噪比,能够在单细胞水平同时定量多个RNA的表达,在获得单细胞中单拷贝RNA表达数据的同时提供完整的组织形态学信息,提高对疾病与标志物之间复杂的生物学相关性的认识,是理想的能够用于NGS和芯片技术后期转化研究技术平台。
自2011年技术推广以来,其应用已在如Nature、Science、NEJM等国际顶级期刊发表超过300篇SCI论文。研究涵盖了感染及免疫、肿瘤、神经生物学、干细胞及发育、非编码RNA、表观遗传学等基础医学领域,以及靶标鉴别和验证、临床前安全性评价和药效评估等药物开发研究。
请访问ACD官网www.acdbio.com。更多中文资料与信息请访问中国官方微信号(ACD_China)咨询。
运动蛋白的介绍
Molecular motor proteins are fascinating enzymes that power much of the movement performed by living organisms. In the first part of this lecture, I will provide an overview of the motors that move along cytoskeletal tracks (kinesin and dynein which move along microtubules and myosin which moves along actin). The main focus of this lecture is on how motor proteins work. How does a nanoscale protein convert energy from ATP hydrolysis into unidirectional motion and force production? What tools do we have at our disposal to study them? The first part of the lecture will focus on these questions for kinesin (a microtubule-based motor) and myosin (an actin-based motor), since they have been the subject of extensive studies and good models for their mechanisms have emerged. I conclude by discussing the importance of understanding motor proteins for human disease, in particular illustrating a recent biotechnology effort from Cytokinetics, Inc. to develop drugs that activate cardiac myosins to improve cardiac contractility in patients suffering from heart failure. The first part of the lecture is directed to a general audience or a beginning graduate class.
In the second part of this lecture, I will discuss our laboratories current work on the mechanism of movement by dynein, a motor protein about which we still know very little. This is a research story in progress, where some advances have been made. However, much remains to be done in order to understand how this motor works.
The third (last) part of the lecture is on mitosis, the process by which chromosomes are aligned and then segregated during cell division. I will describe our efforts to find new proteins that are important for mitosis through a high throughput RNAi screen. I will discuss how we technically executed the screen and then focus on new proteins that are we discovered that are involved in generating the microtubules that compose the mitotic spindle. I also discuss the medical importance of studying mitosis, including the development of drugs targeted to mitotic motor proteins, which are currently undergoing testing in clinical trials.
介绍干细胞及其来源的故事
What are stem cells, where do they come from, and what do we really know about them? An award-winning introduction to the world of stem cell research. Innovative hand-drawn animation, beautiful cell photography and documentary interviews capture the fascination and complexity of this cutting-edge area of science.
RoboSep-S介绍
使用全自动化RoboSep™-S细胞分选平台,简化细胞分选。RoboSep™-S是原热销产品RoboSep™仪器的改进版,实现真正的免操作的同时,保留了EasySep™的快速和简单性,仅需5分钟的手动操作时间。
立迪生物董事长闻丹忆教授详细介绍立迪的一站式肿瘤个性化精准医疗服务平台
上海立迪生物技术股份有限公司董事长兼执行总裁闻丹忆教授以“个性化精准医疗”为主题,向大家介绍了立迪生物的一站式肿瘤个性化精准医疗服务平台。平台可向患者提供的服务包括:患者个体化 PDX 模型保种服务、患者个体化 PDX 模型药物敏感性测试、患者个体化 mini-PDX 药物敏感性测试、患者原代细胞药物敏感性测试(3-D 培养系统)、循环肿瘤细胞分离与检测研究(CTC)、分子靶向药物相关蛋白表达的研究(免疫组化技术)等。更多详细内容,请观看精彩视频。
BaseScope™ 检测技术介绍及应用
参加人员: 对分析任何RNA感兴趣的研究人员,包括具体亚型和新型外显子连接,如可变亚型、高同源性靶标、病毒载体以及密码子优化转基因表达 对验证和定位组织样品中的变异亚型表达感兴趣的qPCR或NGS用户 因抗体对特定靶标(包括高同源性靶标)的可用性而受限制的免疫组化法(IHC)用户 学习目标: BaseScope™检测介绍及其应用概述
单分子超高灵敏度检测技术介绍
默克独创Erenna®单分子免疫检测平台,采用专利单分子检测技术,突破蛋白检测极限,创领生物标志新发现,助力疾病研究再创新。 SMC™技术相较于传统免疫检测技术,信噪比有了很显著的改善,使得在一个系统里可以同时检测低表达和高表达的蛋白靶标,揭示疾病相关生物标志物的微小变化。SMC™技术在经典的三明治免疫夹心原理基础上,通过创见性的洗脱步骤使得荧光素标记的检测抗体从免疫复合物中解离下来。并最终在默克Erenna®系统的毛细管检测空间内以单个分子的形式被激光激发,相应的光学信号被灵敏地记录下来并进行定量计算。检测下限可以下探到1fM。