sRNA Induces the Large-scale Transdetermination of Mesenchymal Stem Cells into Hematopoietic Stem Cells in Human.
Mesenchymal stem cells (MSCs) can differentiate into cells of bone, endothelium, adipose tissue, cartilage, muscle, and brain. However, whether they can transdeterminate into hematopoietic stem cells (HSCs) remains unsolved. We report here that a subpopulation of human MSCs that are CD44+,CD29+, CD105+, CD166+,CD133-,CD34- could differentiate into hematopoietic stem cells (CD150+/CD133+/CD34+) and their descending blood cells in vitro, when transfected with new endogenous shRNAs The sRNA was high-effectively delivered into MSCs by a novel peptide means. These induced MSC-HSCs could form different types of hematopoietic colonies as nature-occurring HSCs did. Upon transplantation into sublethally irradiated NOD/SCID mice, these MSC-HSCs engrafted and differentiated into all hematopoietic lineages such as erythrocytes, lymphocytes, myelocytes and thrombocyte. More importantly, these induced HSCs could successfully engraft and effectively function in patients with severe aplastic anemia. Furthermore, we demonstrated the first evidence that the transdetermination of MSCs was induced by acetylation of histone proteins and activation of many transcriptional factors. Together, our findings identify the sRNAs that dictates a directed differentiation of MSCs toward HSCs and open up a new source for HSCs used for the treatment of blood diseases and artificial stem cell-made blood.
Luminex - xMAP技术优势
该视频通过回答下边两个问题来介绍Luminex技术的优势 Q:Tell us about the luminex technology? Luminex技术简单介绍 Q:How does luminex compare to ELISAs? 与ELISA实验相比Luminex技术具有哪些优势
illumina测序化学原理 - 陈巍学基因（1）
本期课程介绍Illumina测序的化学原理。
包括文库（library）、芯片（flowcell）、桥式PCR、双端测序。
专访Illumina高层：创新引领Illumina前行
生物谷专访了Illumina全球副总裁兼首席商务官Tristan Orpin先生和亚太区总裁Tim Orpin先生，就行业人士广泛关注的罗氏收购Illumina事件、个体化医疗与测序行业发展，Illumina的新产品和技术及市场战略，与这对双胞胎总裁作了深入交流。
Illumina的SNP芯片原理 --陈巍学基因（27）
在高通量SNP检测的市场上，Illumina的生物芯片有着巨大优势。新近很火的美国“23 and ME公司”，就是用的Illumina的Human Omni Express生物芯片来做SNP检测的。
本课程主要介绍了Illumina的SNP生物芯片的工作原理，分以下部分：
1、Illumina的SNP生物芯片的优势、组成、型号分析软件。
2、玻璃基片和微珠的特点和优势。
3、Address序列和 Probe序列的功能与设计。
4、SNP位点的区分方案。
Synthetic Biology & Metabolic Engineering Teaching an Old Bacterium New Tricks
在第一部分，普莱瑟博士解释说，合成生物学涉及工程原理应用到生物的系统建立生物机器。在建筑这些机器的关键材料是合成DNA。合成DNA可以添加在不同的组合，以生物宿主，如细菌，把它们变成化学工厂，能生产小分子的选择。
Synthetic Biology & Metabolic Engineering Teaching an Old Bacterium New Tricks
在2部分，普拉瑟介绍了她的实验室使用的设计原则设计E. coli从葡萄糖生产葡萄糖二酸。葡糖酸不在细菌中自然产生的所以，普拉瑟和她的同事们“bioprospected”从其他生物酶并在大肠杆菌中表达，构建所需的酶途径。普拉瑟走我们通过优化时序的许多步骤，酶表达的定位和水平，以产生最大的产量。
