在染色体分离中有关长度和数量的问题 - Richard McIntosh P1
本视频由科普中国和生物医学大讲堂出品
Richard McIntosh (U. Colorado, Boulder) Part 1: separating Duplicated Chromosomes
The goal of these three talks is to define the problems that a cell faces as it prepares for division and to describe some of the ways it solves them. In Part 1, both the length and amount of DNA are presented as problems for chromosome segregation, particularly in eukaryotic cells. The actions of cohesins and of chromosome condensation are described as solutions. The mitotic machinery is introduced, including its diversity of form across phylogeny, however, the features that appear to be conserved are emphasized. This lecture may be useful for upper level undergraduate and graduate courses discussing mitosis and cell division. see more at www.ibioseminars.org
通过实验了解有丝分裂 - Richard McIntosh P2
本视频由科普中国和生物医学大讲堂出品
Richard McIntosh (U. Colorado, Boulder) Part 2: Understanding Mitosis through Experimentation
The second lecture describes some key experiments showing the dynamics of a formed mitotic spindle and the ways these may contribute to accurate chromosome motion. Experiments that reveal aspects of the processes by which chromosomes attach to the spindle are presented. Mitotic motors are introduced and discussed in the light of what they probably do and do not accomplish to effect chromosome motion, including acting to improve the accuracy of chromosome segregation. see more at http://www.ibioseminars.org
有丝分裂后期：染色体向纺锤体两极移动 - Richard McIntosh P3
本视频由科普中国和生物医学大讲堂出品
Richard McIntosh (U. Colorado, Boulder) Part 3: Moving Chromosome to the spindle Poles: Anaphase A
The third lecture presents evidence, largely from McIntosh's lab, that shows how microtubule depolymerization can move chromosomes in vitro and explores the nature of some of the protein complexes that can couple chromosomes to microtubules and take advantage of this reaction. see more at http://www.ibioseminars.org
基因分裂和RNA剪接 - Melissa Moore P1
本视频由科普中国和生物医学大讲堂出品
Melissa Moore (U. Mass/HHMI) Part 1: split Genes and RNA splicing
In the first part of her talk, Dr. Moore explains that eukaryotic pre-mRNA contains long stretches of non-protein coding sequences interspersed with protein coding regions. By recognizing specific sequences, cellular machinery splices out the non-coding introns leaving just the protein-coding exons in mRNA. Although at first glance this may seem like a wasteful process, it is splicing that facilitates the evolution of new genes, and alternative splicing that allows a limited number of genes to produce a large number of proteins.
病毒包膜的内吞和渗透作用 - Ari Helenius P2
本视频由科普中国和生物医学大讲堂出品
Ari Helenius (ETH Zurich) Part 2: Endocytosis and Penetration
In the second lecture, the next steps in viral infection are described. Endocytosis of plasma membrane bound viruses can occur via a number of mechanisms including caveolar, clathrin, non-clathrin, or lipid raft mediated pathways. The internalized virus is enclosed in an endosome that may undergo increasing acidification resulting in acid mediated fusion between the viral envelope and the vesicle membrane. Following membrane penetration, the virus, once again, makes use of cellular machinery such as microtubules and their motors, to transfer its genome to the nucleus. Helenius describes experiments from his lab and others that have deciphered these complex processes.
用sDs-PAGE技术分离蛋白
十二烷基硫酸钠聚丙烯酰胺凝胶电泳，也称sDs-PAGE，是一种被广泛使用，仅根据分子量大小来分离蛋白质混合物的技术。阴离子去污剂sDs，在变性的线性蛋白表面沿长度均匀分布使其带电。将它们上样到聚丙烯酰胺凝胶后，施加电压，这些表面覆盖sDs的蛋白将被分开。电场作为驱动力，牵引sDs结合的蛋白朝阳极移动，分子量大的蛋白将比小的蛋白移动慢。为了判断蛋白的大小，已知分子量大小的蛋白质标准也会和样品一起上样并在同等条件下跑胶。
本短片介绍sDs-PAGE技术,首先将解释其背后的原理，然后演示每一步的操作过程。视频还将讨论实验中的各种参数，如聚丙烯酰胺浓度，和用于跑胶的电压。还会介绍电泳之后的考马斯亮蓝和银染色方法，以及其他电泳技术，如双向凝胶电泳。
小熊直播第1期：如何赚取Medsci积分
欢迎收看小熊直播课堂节目，今天小熊教大家，如何在“Medsci医学”APP上来赚取积分！
除了购买积分外，总共有以下4种免费获取积分的方式哦~
一.注册即送10个积分
下载打开"Medsci医学"APP，在【我】里面，点击【立即登陆】，在登录页面选择【注册】，完成注册，即可获得10个积分。
官方下载地址：http://www.medsci.cn/m/
二.每日签到领取积分
每天，打开"Medsci医学"APP，在【我】里面，点击【签到+1积分】，即可获得1个积分。
三.资讯评论获得积分
打开"Medsci医学"APP，进入我们的【资讯】页面，评论相关资讯，即可获得2个积分。
四.分享好友送积分
打开"Medsci医学"APP，在【我】里面，点击【邀请好礼】，把里面的【邀请码】分享给好友。好友下载打开APP，输入你的验证码，你即可获得20个积分。
Tagrisso的伴随诊断 - 陈巍学基因(31)
欢迎来到【陈巍学基因】。我们这个节目，主要是介绍基因组学，和临床分子诊断的最新技术进展。
今天，会和大家谈一谈阿斯利康在开发Tagrisso这个新药过程中对4种伴随诊断方法的研究结果。
Tagrisso是阿斯利康公司开发的一个针对EGFR基因有耐药突变的晚期非小细胞肺癌者的药物，是酪氨酸激酶抑制剂类的靶向抗癌药物。
Tagrisso可以选择性地抑制突变型的EGFR，对发生耐药的肿瘤有强抑制作用；而对野生型的EGFR的抑制作用较弱，即对身体正常表皮细胞的生长抑制作用较弱。
下载生物谷APP，观看行云学院视频，让播放更流畅，使用更快捷！
生物谷APP，每天都有新资讯，每天都有好视频！
官方下载地址：http://www.medsci.cn/m/
剪接体的结构和动力学 - Melissa Moore Part 2
本视频由科普中国和生物医学大讲堂出品
本节课Melissa Moore讲述了，剪接体的成分和在每个工作周期中的剪接体。穆尔还解释了如何利用荧光蛋白标签和全反射显微镜的创新，使她和她的同事们能够更好地理解复接机器的有序组装与功能。
Melissa Moore (U. Mass/HHMI) Part 2: spliceosome structure and Dynamics
Moore goes on to describe the cellular splicing machine, the spliceosome, in greater detail in Part 2. she lists the components of the spliceosome and where each works in the spliceosome cycle. Moore also explains how the innovative use of fluorescent protein tags and total internal reflection microscopy has allowed her and her colleagues to better understand the ordered assembly and function of the complex splicing machine.
下载生物谷APP，观看行云学院视频，让播放更流畅，使用更快捷！
生物谷APP，每天都有新资讯，每天都有好视频！
官方下载地址：http://www.medsci.cn/m/
我是如何成为一名科学家的 - Alfredo Quinones-Hinojosa
本视频由科普中国和生物医学大讲堂出品
Alfredo Quinones-Hinojosa（Q博士）（霍普金斯达大学）：我是如何成为一名科学家
出生于墨西哥的Q博士，19岁时翻越美国边界的栅栏，成为加利福尼亚的一名农场工人。由于他自己的决心，努力工作和自律，以及来自家庭和朋友的大量支持，他离开了农场工作，完成了大学和医学院的学业，最终成为一位非常成功的医生、科学家和脑外科医生。
关于讲师：Alfredo Quinones-Hinojosa是约翰霍普金斯大学，神经外科学和肿瘤学，神经系统的细胞与分子医学的副教授，脑肿瘤外科手术的主任。Q博士作为一个专家外科医生，他致力于干细胞，在脑肿瘤的病因和治疗领域的研究。
Alfredo Quinones-Hinojosa (Dr. Q) (Johns Hopkins): How I Became a scientist
At age 19, Quiñones-Hinojosa jumped the fence from Mexico to become a farm worker in California. Thanks to his own determination, hard work and discipline and a lot of support from family and friends, he left farm work, completed university and medical school, and ultimately became a highly successful physician-scientist and brain surgeon.
About the speaker: Alfredo Quiñones-Hinojosa is an Associate Professor of Neurosurgery and Oncology, Neuroscience and Cellular and Molecular Medicine and Director of the Brain Tumor surgery Program at Johns Hopkins University. As well as being an expert surgeon, he researches the role of stem cells both in causing brain tumors and potentially in fighting them.
This talk was first released in iBioMagazine Issue 5.
下载生物谷APP，观看行云学院视频，让播放更流畅，使用更快捷！
生物谷APP，每天都有新资讯，每天都有好视频！
官方下载地址：http://www.medsci.cn/m/