Capp移液器
Capp移液器,中文音译为“凯博”系隶属于丹麦Capp Aps 公司,拥有世界上唯一的48道、64道移液器,其单道移液成为移液器领域内唯一一款可以在固定量程和可变量程之间任意转换的移液器。
Targets-based therapy for leukemia: opportunity and challenge
优点主要为:能增强患者的免疫力,防止肿瘤的转移和复发,对病人机体的损伤小。 在我国,现在普遍开展的树突状细胞(DC)和细胞因子诱导的杀伤细胞(CIK)的生物疗法被广泛应用。
Simultaneous quantification of 47 gene expression in FFPE samples by a novel PCR-free approach
基因表达(gene expression)是指细胞在生命过程中,把储存在DNA顺序中遗传信息经过转录和翻译,转变成具有生物活性的蛋白质分子。生物体内的各种功能蛋白质和酶都是同相应的结构基因编码的。差别基因表达(differential gene expression)指细胞分化过程中,奢侈基因按一定顺序表达,表达的基因数约占基因总数的5%~10%。
金颖:Fox3 suppresses NFAT-mediated differentiation to maintain self-renewal of embryonic stem cells
金颖教授为分子发育生物学研究室主任,健康科学中心研究员。金教授介绍了Fox3通过抑制NFAT介导的分化维持了胚胎干细胞的自我更新的机制等前沿发现。
Pluripotency-associated transcription factor Foxd3 is required for maintaining pluripotent cells. However, molecular mechanisms underlying its function are largely unknown.
Here, we report that Foxd3 suppresses differentiation induced by Calcineurin-NFAT signaling to maintain the ESC identity. Mechanistically, Foxd3 interacts with NFAT proteins and recruits co-repressor Tle4, a member of the Tle suppressor family highly expressed in undifferentiated ESCs, to repress NFATc3’s transcriptional activities.
Furthermore, global transcriptome analysis shows that Foxd3 and NFATc3 co-regulate a set of differentiation-associated genes in ESCs. Collectively, our study establishes a molecular and functional link between a pluripotency-associated factor and an important ESC differentiation-inducing pathway.
GE:CIpp蛋白纯化策略三部曲
小改变,大不同!主动出击,科学设计层析流程,革命性优化试验结果。本期网络讲堂我们将介绍CIpp:CIpp是经过证实的可靠蛋白纯化策略。我们将层析过程分为三个不同阶段:捕获( Capture)、中度纯化( intermediate Purification)、精细纯化( Polishing),即CIpp。不同阶段目的是什么?不同阶段如何选择层析技术?各种层析技术如何衔接?我们都将一一阐述。
PureBlu™ Hoechst 33342 Nuclear Staining Dye for Live Cells - A Fast Approach to Staining Nuclei
This brief tutorial demonstrates the use of the PureBlu Hoechst 33342 Dye with the ZOE™ Fluorescent Cell Imager for routine nuclear staining in fluorescence microscopy and cell imaging applications.
李晓涛:蛋白酶体激活因子REGγ在实验性肠炎中调控NF-KappaB的作用与机制
介绍了去乙酰化酶在细胞代谢中相关调控活性。提到了SENP1、SUMOylation and De- SUMOylation等