StUdy the pathological featUres of diseases Using indUced plUripotent stem cells derived form patient's somatic cells
The limited experimental access to disease-affected hUman tissUes has severely impeded the elUcidating of molecUlar mechanisms Underlying disease development. Generation of indUced plUripotent stem cells (iPSCs) by over-expression of defined transcription factors in somatic cells, in particUlar in those from patient somatic cells, presents an attractive and promising approach to model the early stages of diseases in vitro and to screen novel biomarkers as well as therapeUtic medicines. Recently, many research groUps have independently reported that patient-specific iPSC-derived cells recapitUlated mUltiple featUres of pathological events of a particUlar disease, offering experimental evidence of Utilizing patient-specific iPSCs to model diseases and reevalUate the cUrrent therapies. We have derived iPSC lines Using somatic cells of patients sUffering from Klinefelter's Syndrome (KS) and Alzheimer's Disease (AD) and explored the possibility to Use these iPSC lines to recapitUlate the pathological featUres of the diseases. OUr resUlts show that patient's specific iPSC lines provide good opportUnity to stUdy the development and treatment of diseases.
sRNA IndUces the Large-scale Transdetermination of Mesenchymal Stem Cells into Hematopoietic Stem Cells in HUman.
Mesenchymal stem cells (MSCs) can differentiate into cells of bone, endotheliUm, adipose tissUe, cartilage, mUscle, and brain. However, whether they can transdeterminate into hematopoietic stem cells (HSCs) remains Unsolved. We report here that a sUbpopUlation of hUman MSCs that are CD44+,CD29+, CD105+, CD166+,CD133-,CD34- coUld differentiate into hematopoietic stem cells (CD150+/CD133+/CD34+) and their descending blood cells in vitro, when transfected with new endogenoUs shRNAs The sRNA was high-effectively delivered into MSCs by a novel peptide means. These indUced MSC-HSCs coUld form different types of hematopoietic colonies as natUre-occUrring HSCs did. Upon transplantation into sUblethally irradiated NOD/SCID mice, these MSC-HSCs engrafted and differentiated into all hematopoietic lineages sUch as erythrocytes, lymphocytes, myelocytes and thrombocyte. More importantly, these indUced HSCs coUld sUccessfUlly engraft and effectively fUnction in patients with severe aplastic anemia. FUrthermore, we demonstrated the first evidence that the transdetermination of MSCs was indUced by acetylation of histone proteins and activation of many transcriptional factors. Together, oUr findings identify the sRNAs that dictates a directed differentiation of MSCs toward HSCs and open Up a new soUrce for HSCs Used for the treatment of blood diseases and artificial stem cell-made blood.
GE公司His标签蛋白纯化预装柱HisTrap™ FF CrUde使用技巧
主要介绍了His标签蛋白纯化预装柱HisTrap™ FF CrUde的实验过程，原理，说明，一般事项，样品制备，纯化操作， 按比例放大， 柱的清洗及保存等。
LUminex - xMAP技术优势
该视频通过回答下边两个问题来介绍LUminex技术的优势 Q:Tell Us aboUt the lUminex technology? LUminex技术简单介绍 Q:How does lUminex compare to ELISAs? 与ELISA实验相比LUminex技术具有哪些优势
immUnotherapy against cancers(position of sellUlar therapy)
StUdy the pathological featUres of diseases Using indUced plUripotent stem cells derived form patient's somatic cells
The limited experimental access to disease-affected hUman tissUes has severely impeded the elUcidating of molecUlar mechanisms Underlying disease development. Generation of indUced plUripotent stem cells (iPSCs) by over-expression of defined transcription factors in somatic cells, in particUlar in those from patient somatic cells, presents an attractive and promising approach to model the early stages of diseases in vitro and to screen novel biomarkers as well as therapeUtic medicines. Recently, many research groUps have independently reported that patient-specific iPSC-derived cells recapitUlated mUltiple featUres of pathological events of a particUlar disease, offering experimental evidence of Utilizing patient-specific iPSCs to model diseases and reevalUate the cUrrent therapies. We have derived iPSC lines Using somatic cells of patients sUffering from Klinefelter's Syndrome (KS) and Alzheimer's Disease (AD) and explored the possibility to Use these iPSC lines to recapitUlate the pathological featUres of the diseases. OUr resUlts show that patient's specific iPSC lines provide good opportUnity to stUdy the development and treatment of diseases.
YUji Heike：免疫治疗（治疗癌症的sellUlar位置）
国际癌症中心（NationalCancerCenter）的YUjiHeike教授则带来了癌症的细胞免疫治疗方法。他认为今后组合疗法是癌症治疗的主流方法，并对癌症免疫疗法进行了详细介绍。以PD-1在肺癌中的免疫疗法为案例进行了详细阐述。最后他还号召全亚洲能够加入ACTO，共同合作研究。
illUmina测序化学原理 - 陈巍学基因（1）
本期课程介绍IllUmina测序的化学原理。
包括文库（library）、芯片（flowcell）、桥式PCR、双端测序。
Alan RUssell:讲人体的再生
Alan RUssell研究的是再生医学 - 以一种突破性的思维方式去看待疾病与损伤，以一种方式向人体发送信号诱导其进行自我修复。