GE公司His标签蛋白纯化预装柱HiSTRap™ FF Crude使用技巧
主要介绍了His标签蛋白纯化预装柱HiSTRap™ FF Crude的实验过程,原理,说明,一般事项,样品制备,纯化操作, 按比例放大, 柱的清洗及保存等。
NanoSTRing--陈巍学基因(19)
本节课程向大家谈一下NanoSTRing的分析方法。
从以下方面进行阐述:
NanoSTRing的优点
NanoSTRing的化学原理
Controlling the Cell Cycle: Cdk SubSTRates - David O. Morgan
本视频由科普中国和生物医学大讲堂出品
David O. Morgan (UCSF) Part 2: Controlling the Cell Cycle: Cdk SubSTRates
Cyclin-dependent kinases (Cdks) are the central components of the control system that initiates the events of the cell cycle. In the second part of this lecture, I discuss my laboratory's efforts to address the problem of how the Cdks trigger cell-cycle events. I describe our methods for identifying the protein subSTRates of the Cdks, and I discuss how these studies have led to important clues about how Cdks find their correct targets in the cell and how phosphorylation of those targets governs their function.