InterPLAys between Mesenchymal Stem Cells and Immune Responses
Mesenchymal stem cells (MSCs) exist in almost all tissues and are crucial in maintaining the cellular homeostasis of multicellular organisms. They provide the ultimate cell source for tissue repair and regeneration. Under pathological conditions, these cells are awakened, activated, and mobilized to damaged tissue sites. Since tissue damages are often accompanied by inflammatory factors, from both innate immune response and adaptive immune response, it is sensible that MSCs delicately interact with inflammatory factors at the sites of tissue damages.
Depending on the type and persistence of the inflammatory factors, the activated MSCs could lead either to complete or partial tissue repair, or to chronic inflammation and further tissue damage, such as cancer and fibrosis. Indeed, recent studies have shown that there is a bidirectional interaction between MSCs and inflammatory cells and cytokines. However, much information of this information remains to be elucidated. Further investigations in this newly emerging exciting research area will undoubtedly lead to better understanding of pathogenesis of various diseases and novel treatment strategies.
Duolink® PLA® 技术——加速信号通路发现过程
Duolink® 实验基于近位连接技术(Proximity Ligation Assay, PLA)，当一对PLA probes足够接近时(<40 nm)会产生PLA信号，信号的强弱直接反映了蛋白表达量水平或蛋白互作的强度。Duolink® 实验可在细胞生理状态下，定位检测蛋白-蛋白互作、蛋白表达或蛋白翻译后修饰，检测结果更精确、真实、可靠。
Duolink实验——利用PLA技术检测Smad复合体
BMP4_Smad信号通路具有非常重要的作用，研究表明BMP4通过Smad1/5/8信号通路可促进哺乳动物卵原干细胞分化。该课程详细讲述了通过原位PLA技术可检测到BMP4信号转导下游效应因子Smad1/5/8与Smad4形成复合体，进而激活一系列基因的转导。
Introducing the EnSight™ Multimode PLAte Reader from PerkinElmer
PerkinElmer's EnSight Multimode PLAte Reader is the first benchtop system to offer well-imaging alongside label-free and labeled detection technologies - for a whole new perspective on your research. For more information, please visit the EnSight website - http://bit.ly/T4IDPh
CytoPLAsm是什么？
Margaret Gardel探讨细胞质的物理性质，一个复杂的流体内的细胞，是挤满了蛋白质，细胞器和丝状网络。她沉思，例如，运动蛋白如何克服身体携带的货物的阻力，以及如何细胞进行形状的变化，以应对不同的刺激。为了帮助解决这些问题，她提出了一个“新物理学”。
看得见的蛋白互作-Duolink PLA实验操作视频
Duolink® 实验基于邻位连接技术(Proximity Ligation Assay, PLA),可将蛋白信号放大1000倍，实现单分子级别检测灵敏度。即使是微量样本、微弱互作、极罕见低丰度表达，也能在细胞生理水平下，获得基于图像的可视化蛋白检测结果，真实反映内源表达水平蛋白的互作、定位和定量，结果可靠有说服力。该视频详细介绍了Duolink实验的每步操作，请跟随Tracy Adair-Kirk博士，一起开启Duolink® PLA®实验之旅。