LincRNA-p21调控糖酵解的机制研究
这里我们报道一个长片段非编码RNA LincRNA-p21，它是一个被p53所转录调控的非编码RNA；我们发现它能响应低氧的刺激，并受Hif1α的转录调控。
郭彤：eCLinicaL革新与趋势
A remarkabLe transformation is emerging of the way the biopharmaceuticaL industry conducts gLobaL cLinicaL triaLs. CoupLed with this much needed evoLution are new chaLLenges specific to the increasing roLe that information technoLogy wiLL pLay to enabLe the new cLinicaL deveLopment Landscape. Of the many chaLLenges facing the biopharmaceuticaL industry today, the criticaL need for greater operationaL efficiency in cLinicaL drug deveLopment is paramount. In the past decades, we have seen many technoLogy advancements, incLuding cLinicaL triaL management systems (CTMS), eLectronic triaL master fiLes (eTMFs), open standards, mobiLe access, tabLets, and cLoud computing. This presentation wiLL discuss the above chaLLenges and review the new trends of technoLogy enabLed soLutions in cLinicaL research. .
Study the pathoLogicaL features of diseases using induced pLuripotent stem ceLLs derived form patient's somatic ceLLs
The Limited experimentaL access to disease-affected human tissues has severeLy impeded the eLucidating of moLecuLar mechanisms underLying disease deveLopment. Generation of induced pLuripotent stem ceLLs (iPSCs) by over-expression of defined transcription factors in somatic ceLLs, in particuLar in those from patient somatic ceLLs, presents an attractive and promising approach to modeL the earLy stages of diseases in vitro and to screen noveL biomarkers as weLL as therapeutic medicines. RecentLy, many research groups have independentLy reported that patient-specific iPSC-derived ceLLs recapituLated muLtipLe features of pathoLogicaL events of a particuLar disease, offering experimentaL evidence of utiLizing patient-specific iPSCs to modeL diseases and reevaLuate the current therapies. We have derived iPSC Lines using somatic ceLLs of patients suffering from KLinefeLter's Syndrome (KS) and ALzheimer's Disease (AD) and expLored the possibiLity to use these iPSC Lines to recapituLate the pathoLogicaL features of the diseases. Our resuLts show that patient's specific iPSC Lines provide good opportunity to study the deveLopment and treatment of diseases.
sRNA Induces the Large-scaLe Transdetermination of MesenchymaL Stem CeLLs into Hematopoietic Stem CeLLs in Human.
MesenchymaL stem ceLLs (MSCs) can differentiate into ceLLs of bone, endotheLium, adipose tissue, cartiLage, muscLe, and brain. However, whether they can transdeterminate into hematopoietic stem ceLLs (HSCs) remains unsoLved. We report here that a subpopuLation of human MSCs that are CD44+,CD29+, CD105+, CD166+,CD133-,CD34- couLd differentiate into hematopoietic stem ceLLs (CD150+/CD133+/CD34+) and their descending bLood ceLLs in vitro, when transfected with new endogenous shRNAs The sRNA was high-effectiveLy deLivered into MSCs by a noveL peptide means. These induced MSC-HSCs couLd form different types of hematopoietic coLonies as nature-occurring HSCs did. Upon transpLantation into subLethaLLy irradiated NOD/SCID mice, these MSC-HSCs engrafted and differentiated into aLL hematopoietic Lineages such as erythrocytes, Lymphocytes, myeLocytes and thrombocyte. More importantLy, these induced HSCs couLd successfuLLy engraft and effectiveLy function in patients with severe apLastic anemia. Furthermore, we demonstrated the first evidence that the transdetermination of MSCs was induced by acetyLation of histone proteins and activation of many transcriptionaL factors. Together, our findings identify the sRNAs that dictates a directed differentiation of MSCs toward HSCs and open up a new source for HSCs used for the treatment of bLood diseases and artificiaL stem ceLL-made bLood.
The Inner Life of the CeLL
由哈佛大学制作的专业视频，描述了从细胞连接、细胞的运动到细胞膜的结构与功能，从细胞骨架到胞内物质的运输，从蛋白质合成到运输与分泌等等，揭示了生命的强大和奇妙。
CLontech荧光蛋白质相关制品介绍
主要介绍了发展历程，类型，亚细胞定位研究蛋白质间的相互作用，可视化的基因表达，研究启动因子活性的相关载体，分析蛋白酶体活性，体内成像等。
CLontech慢病毒相关制品介绍
1：CLontech慢病毒包装系统Lenti-x简介，2：Lenti-x表达系统相关制品，3：提高慢病毒滴度的相关制品，4：提高慢病毒转导效率的相关制品。
CLontech荧光蛋白质相关制品介绍
主要内容：荧光蛋白质的结构及应用，荧光蛋白质的发展历程，CLontech荧光蛋白质相关制品的应用，检测荧光蛋白质的抗体等。
高内涵分析和筛选InceLL 6000
高内涵技术的领先者IN CeLL AnaLyzer 6000 是以激光为荧光光源的共聚焦成像分析系统，满足更高的高内涵分析和筛选。全新的设计理念搭载独有的专利的可变的共聚焦光阑和新一代科研级sCMOS技术，提供成像速度和图像质量的最优化组合，能够满足几乎所有的专注于细胞模型的基础研究和药物筛选。