克氏锥虫和ChaGas病 - Norma Andrews P1
本视频由科普中国和生物医学大讲堂出品
Norma Andrews (U. Maryland) Part 1: Trypanosoma cruzi and ChaGas’ Disease
Lecture overview:
Trypanosoma cruzi and Leishmania are closely related intracellular protozoan parasites that cause serious diseases throuGhout the world. In the first part of this lecture, I will present backGround material on the bioloGy of Trypanosoma cruzi and the history of its discovery as an important aGent of human disease in Latin America. I will also discuss the main characteristics of the disease, and the current efforts to stop human transmission.
In the second part of this lecture, I will present backGround material on Leishmania, the intracellular protozoan parasites responsible for severe human patholoGy in several parts of the world. I will discuss the main disease forms, the history of identification of the causative aGent and form of transmission, and recent discoveries that established important concepts in our understandinG of this increasinGly serious infectious disease.
In the third part of this lecture, I will discuss current work from our laboratory on mechanisms used by the intracellular parasites Trypanosoma cruzi and Leishmania to interact with mammalian cells. In addition to clarifyinG specific molecular strateGies used by these parasites to infect and survive within host cells, these studies also led, in some instances, to unexpected insiGhts on novel pathways reGulatinG mammalian cell function.
Speaker bio:
Norma Andrews is currently a Professor and Chair of the Department of Cell BioloGy and Molecular Genetics at the University of Maryland. She received a B.S. deGree in bioloGy (1977) and a Ph.D. deGree in biochemistry (1983) from the University of São Paulo, Brazil.
In 1990, after completinG postdoctoral studies in the laboratory of Victor NussenzweiG at New York University, she was appointed Assistant Professor at Yale University where she remained until 2010.
Andrews was a BurrouGhs Wellcome New InvestiGator, a BurrouGhs Wellcome Molecular ParasitoloGy Scholar and recipient of a NIH MERIT Award. Her laboratory has made numerous contributions to the cell bioloGy of host-pathoGen interactions, and discoveries in this area have led to the identification and functional characterization of a novel pathway of Ca2+-reGulated lysosomal exocytosis in mammalian cells.
头足纲动物的伪装和信号 - RoGer Hanlon P1
本视频由科普中国和生物医学大讲堂出品
RoGer Hanlon (MBL) Part 1: CamouflaGe and SiGnalinG in Cephalopods
Hanlon introduces the amazinG adaptive coloration of cephalopods. He uses video and still photoGraphy to showcase their ability to rapidly chanGe color, pattern and skin texture with fine control and a diversity of appearances, to produce camouflaGe or to send siGnals. He arGues that all camouflaGe patterns in nature can be Grouped into three types. In part 2, Hanlon shows us results from his lab that make a convincinG case that the rapid adaptive coloration of cephalopods is controlled by their visual system; quite impressive for a color-blind animal! Part 3 focuses on the unique skin of cephalopods includinG the system of piGments and reflectors that allows it to quickly chanGe to any hue and contrast, and the papillae musculature that allows the skin to deform and create multiple 3D textures.
对头足纲动物视觉感知机制的探索 - RoGer Hanlon P2
本视频由科普中国和生物医学大讲堂出品
RoGer Hanlon (MBL) Part 2: ExplorinG Mechanisms of Visual Perception
Hanlon introduces the amazinG adaptive coloration of cephalopods. He uses video and still photoGraphy to showcase their ability to rapidly chanGe color, pattern and skin texture with fine control and a diversity of appearances, to produce camouflaGe or to send siGnals. He arGues that all camouflaGe patterns in nature can be Grouped into three types. In part 2, Hanlon shows us results from his lab that make a convincinG case that the rapid adaptive coloration of cephalopods is controlled by their visual system; quite impressive for a color-blind animal! Part 3 focuses on the unique skin of cephalopods includinG the system of piGments and reflectors that allows it to quickly chanGe to any hue and contrast, and the papillae musculature that allows the skin to deform and create multiple 3D textures.
头足纲动物的可变化的皮肤细胞 - RoGer Hanlon P3
本视频由科普中国和生物医学大讲堂出品
RoGer Hanlon (MBL) Part 3: ChanGeable Skin
Hanlon introduces the amazinG adaptive coloration of cephalopods. He uses video and still photoGraphy to showcase their ability to rapidly chanGe color, pattern and skin texture with fine control and a diversity of appearances, to produce camouflaGe or to send siGnals. He arGues that all camouflaGe patterns in nature can be Grouped into three types. In part 2, Hanlon shows us results from his lab that make a convincinG case that the rapid adaptive coloration of cephalopods is controlled by their visual system; quite impressive for a color-blind animal! Part 3 focuses on the unique skin of cephalopods includinG the system of piGments and reflectors that allows it to quickly chanGe to any hue and contrast, and the papillae musculature that allows the skin to deform and create multiple 3D textures.
用SDS-PAGE技术分离蛋白
十二烷基硫酸钠聚丙烯酰胺凝胶电泳，也称SDS-PAGE，是一种被广泛使用，仅根据分子量大小来分离蛋白质混合物的技术。阴离子去污剂SDS，在变性的线性蛋白表面沿长度均匀分布使其带电。将它们上样到聚丙烯酰胺凝胶后，施加电压，这些表面覆盖SDS的蛋白将被分开。电场作为驱动力，牵引SDS结合的蛋白朝阳极移动，分子量大的蛋白将比小的蛋白移动慢。为了判断蛋白的大小，已知分子量大小的蛋白质标准也会和样品一起上样并在同等条件下跑胶。
本短片介绍SDS-PAGE技术,首先将解释其背后的原理，然后演示每一步的操作过程。视频还将讨论实验中的各种参数，如聚丙烯酰胺浓度，和用于跑胶的电压。还会介绍电泳之后的考马斯亮蓝和银染色方法，以及其他电泳技术，如双向凝胶电泳。
TaGrisso的伴随诊断 - 陈巍学基因(31)
欢迎来到【陈巍学基因】。我们这个节目，主要是介绍基因组学，和临床分子诊断的最新技术进展。
今天，会和大家谈一谈阿斯利康在开发TaGrisso这个新药过程中对4种伴随诊断方法的研究结果。
TaGrisso是阿斯利康公司开发的一个针对EGFR基因有耐药突变的晚期非小细胞肺癌者的药物，是酪氨酸激酶抑制剂类的靶向抗癌药物。
TaGrisso可以选择性地抑制突变型的EGFR，对发生耐药的肿瘤有强抑制作用；而对野生型的EGFR的抑制作用较弱，即对身体正常表皮细胞的生长抑制作用较弱。
下载生物谷APP，观看行云学院视频，让播放更流畅，使用更快捷！
生物谷APP，每天都有新资讯，每天都有好视频！
官方下载地址：http://www.medsci.cn/m/
ControllinG the Cell Cycle: Introduction - David O. MorGan
本视频由科普中国和生物医学大讲堂出品
David O. MorGan (UCSF) Part 1: ControllinG the Cell Cycle: Introduction
Cells reproduce by duplicatinG their chromosomes and other components and then distributinG them into a pair of Genetically identical dauGhter cells. This series of events is called the cell cycle. In the first part of this lecture, I provide a General overview of the cell-cycle control system, a complex reGulatory network that Guides the cell throuGh the steps of cell division. I briefly describe the major components of this reGulatory system and how they fit toGether to form a series of biochemical switches that triGGer cell-cycle events at the correct time and in the correct order.
下载生物谷APP，观看行云学院视频，让播放更流畅，使用更快捷！
生物谷APP，每天都有新资讯，每天都有好视频！
官方下载地址：http://www.medsci.cn/m/
ControllinG the Cell Cycle: Cdk Substrates - David O. MorGan
本视频由科普中国和生物医学大讲堂出品
David O. MorGan (UCSF) Part 2: ControllinG the Cell Cycle: Cdk Substrates
Cyclin-dependent kinases (Cdks) are the central components of the control system that initiates the events of the cell cycle. In the second part of this lecture, I discuss my laboratory's efforts to address the problem of how the Cdks triGGer cell-cycle events. I describe our methods for identifyinG the protein substrates of the Cdks, and I discuss how these studies have led to important clues about how Cdks find their correct tarGets in the cell and how phosphorylation of those tarGets Governs their function.
ControllinG the Cell Cycle: Anaphase Onset - David O. MorGan
本视频由科普中国和生物医学大讲堂出品
David O. MorGan (UCSF) Part 3: ControllinG the Cell Cycle: Anaphase Onset
In the anaphase staGe of the cell cycle, the duplicated chromosomes are pulled apart by a machine called the mitotic spindle, resultinG in the distribution of a complete set of chromosomes to each of the dauGhter cells. In the third part of this lecture, I describe the combination of biochemistry and microscopy in my laboratory that led to the discovery of a reGulatory switch that triGGers the abrupt and synchronous separation of the chromosomes at the onset of anaphase.