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GE：2016第一弹之蛋白样品制备

蛋白样品制备是进行样品分析前的第一步，制备的质量对于成功进行蛋白分析来说至关重要。本次内容主要从蛋白收集提取、亲和富集、准确定量到脱盐除杂进行讲解，努力为在蛋白质分析研究领域入门或探索阶段的科研人员提供完整的解决方案。

从古希腊到21世纪，对多细胞生物中的再生能力的研究

Alejandro Sanchez-Alvarado (Stowers Institute for Medical Research) Part 1: History of Regeneration

Regeneration has fascinated philosophers and scientists since the beginning of history. The wide but uneven distribution of regenerative capacities among multicellular organisms is puzzling, and the permissive/inhibitory mechanisms regulating this attribute in animals remain a mystery. In the first part of this lecture, I will provide a general history of regeneration research from ancient Greece to the beginning of the 20th century. Key concepts will be introduced in their appropriate historical context, and many of the unanswered questions put forward by the problem of regeneration will be discussed.

Alejandro Sánchez Alvarado moved from the University of Utah to the Stowers Institute for Medical Research in 2011.

2016第二弹之细胞治疗中病毒载体的下游纯化

近年来，以CAR-T为代表的的特异性细胞免疫治疗为攻克癌症带来了一丝曙光，越来越多的创业公司及科研单位纷纷投身于该领域，方兴未艾！在细胞治疗整个流程中涉及细胞分选，培养，质粒及病毒包装纯化等，高品质的病毒载体在CAR-T疗法中至关重要。病毒载体的纯化方式可分为：超滤法，超高速离心法以及层析法等。其中，层析法对设备要求低，具有时间短，通量高，纯度高和易于放大的特点，是一种非常重要的病毒载体纯化手段。在本次报告中，我们将讨论如何利用不同的层析介质以及合理的衔接步骤对病毒载体进行纯化。

10种能够永生的动物

科学家们研究这10种长寿的动物，打算让人类也能获得它们的能力。其中，包括了水熊虫和灯塔水母，这些bug一样的生物。试想，一个可以返老还童，而且是无限循环；另一个抗辐射、耐高温，能在几乎一切恶劣的环境中生存。如果我能揭露了这些生物存在的秘密，我将完爆苹果、谷歌、微软、etc，这些曾今的寡头企业。算了，洗洗睡吧，明早起来还要去搬砖呢~

Western Blot 第1阶段：样品制备

Novus Biologicals Visual Protocols: In phase 1 of the western blot procedure, you will learn how to prepare your samples before loading them into a gel. Here we isolate protein from cultured cells, quantify total protein concentrations with a BCA assay, add loading buffer to the sample, and heat the sample. Additional help can be found in the support section of http://www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.

荧光免疫细胞化学检测法（ICC）- 第1部分

In this video you will learn how to clean and coat coverslips, prepare cell culture plates, and plate cells for ICC.

PKI Mantra Intro 2015

The Mantra™ quantitative pathology workstation lets you visualize, quantify, and phenotype multiple types of immune cells simultaneously in intact FFPE tissue sections for cancer immunology research. Mantra allows you to maintain tissue morphology to help better understand the types and roles of cells within the tumor and tumor microenvironment. The system comes with powerful inForm® Tissue Finder™ advanced image analysis software that features user-trainable algorithms for automatic recognition of specific tissue and cell types, as well as per-cell analysis of biomarker expression and phenotyping to differentiate among cell types. Pathology Views™ creates familiar-looking brightfield (H&E, DAB, hematoxylin) renderings from original multi-label fluorescence images. PerkinElmer’s cancer immunology workflow has been validated using Opal™ reagent kits. Opal methodology enables specific staining of multiple tissue biomarkers reaching up to 6-plex and beyond in a single tissue section. Learn more: http://www.perkinelmer.com/catalog/pr...

【GE技术大咖秀】2016第三弹之重组标签蛋白纯化策略及方法

本次讲解将与大家一起分享关于重组标签蛋白的纯化策略以及可能会涉及到的一些常用的技术方法。内容主要分为两部分：首先与大家一起了解一下什么是重组标签蛋白，他的表达和纯化的基本流程有哪些，一般会采用什么样的方式进行纯化。接下来，与大家分享一些重组标签蛋白纯化方面的新的解决方案。