大脑是怎样帮我们定位的
你是如何回忆起将车停在了什么地方呢?你怎么判断自己是否走对了方向呢?神经系统科学家尼尔•伯吉斯(Neil Burgess)研究了神经系统的定位功能原理,以及该原理与记忆和想象的关系。
啮齿动物的立体定位手术
立体定向(或立体)手术是一种用来操作活体动物大脑的方法。该技术使得研究人员能够通过使用立体定位图谱准确地定位大脑内的深层结构。立体定位图谱提供了每个脑区相对于颅骨上的解剖学标志的三维坐标。将颅骨暴露后,麻醉的动物被固定在被称为立体定位仪的专门仪器上,该仪器能使实验工具精确放置在定好的坐标上。立体定位手术是一项用途广泛的技术,可用来产生损伤,操纵基因表达,或将实验试剂施加到大脑里。
本视频文章概述了立体定位手术的原理,包括使用立体定位图谱和立体定位仪的说明,并介绍了读取测量探头移动的游标刻度的方法。随后的讨论概括了手术过程所需的步骤。最后,展示了该技术的广泛应用,如插入电极探针以测量大脑活动以及脑组织的遗传操作方法。
蛋白激酶的调控与定位- Susan Taylor
In this lecture, I have given an overview of protein kinase structure and function using cyclic AMP dependent kinase (PKA) as a prototype for this enzyme superfamily. I have demonstrated what we have learned from the overall structural kinome which allows us to compare many protein kinases and also to appreciate how the highly regulated eukaryotic protein kinase has evolved. By comparing many protein kinase structures, we are beginning to elucidate general rules of architecture. In addition, I have attempted to illustrate how PKA is regulated by cAMP and how it is localized to specific macromolecular complexes through scaffold proteins.