Study the pathological features of diseases using induced pluripotent stem cells derived form patient's somatic cells
The limited experimental access to disease-affected human tissues has severely impeded the elucidating of molecular mechanisms underlying disease development. Generation of induced pluripotent stem cells (iPSCs) by over-expression of defined transcription factors in somatic cells, in particular in those from patient somatic cells, presents an attractive and promising approach to model the early stages of diseases in vitro and to screen novel biomarkers as well as therapeutic medicines. Recently, many research groups have independently reported that patient-specific iPSC-derived cells recapitulated multiple features of pathological events of a particular disease, offering experimental evidence of utilizing patient-specific iPSCs to model diseases and reevaluate the current therapies. We have derived iPSC lines using somatic cells of patients suffering from Klinefelter's Syndrome (KS) and Alzheimer's Disease (AD) and explored the possibility to use these iPSC lines to recapitulate the pathological features of the diseases. Our results show that patient's specific iPSC lines provide good opportunity to study the development and treatment of diseases.
SIMDol健康云服务及可穿戴医疗设备
自主知识产权、技术领先、专业门槛；创新的医疗健康管理模式：团队二三十年行业经验，稀缺资源；开放的服务平台；适用亿万级用户市场规模；持续增值的专业管理方法（模型）。中国至少有3~5亿适用人群，若以20%的市场购买率作为目标市场预测，将会有上亿规模的客户市场。本项目保守预测增速在60%以上，达产后的营收可达数亿元。
Study the pathological features of diseases using induced pluripotent stem cells derived form patient's somatic cells
The limited experimental access to disease-affected human tissues has severely impeded the elucidating of molecular mechanisms underlying disease development. Generation of induced pluripotent stem cells (iPSCs) by over-expression of defined transcription factors in somatic cells, in particular in those from patient somatic cells, presents an attractive and promising approach to model the early stages of diseases in vitro and to screen novel biomarkers as well as therapeutic medicines. Recently, many research groups have independently reported that patient-specific iPSC-derived cells recapitulated multiple features of pathological events of a particular disease, offering experimental evidence of utilizing patient-specific iPSCs to model diseases and reevaluate the current therapies. We have derived iPSC lines using somatic cells of patients suffering from Klinefelter's Syndrome (KS) and Alzheimer's Disease (AD) and explored the possibility to use these iPSC lines to recapitulate the pathological features of the diseases. Our results show that patient's specific iPSC lines provide good opportunity to study the development and treatment of diseases.
Eric Topol:未来医疗的无线化
EricTopol说，我们很快就可以通过智能手机来监控我们的生命体征并监测一些慢性疾病。在TEDMED上，他着重介绍了应用在未来医疗上的一些重要无线装置，这些装置可以帮助我们远离医院的病床。
Ben Goldacre：医生开药的盲点所在
当一种新型药物被测试的时候，试验结果应该对其余医药界开放 — 但很多时候， 负面或者无效的结论未被上报，导致医生和研究人员对此一无所知。在这激情洋溢的演讲中，Ben Goldacre讲述了为什么这些未上报的负面的案例会误导大众，并有潜在的危险。
Duolink® PLA® 技术——加速信号通路发现过程
Duolink® 实验基于近位连接技术(Proximity Ligation Assay, PLA)，当一对PLA probes足够接近时(<40 nm)会产生PLA信号，信号的强弱直接反映了蛋白表达量水平或蛋白互作的强度。Duolink® 实验可在细胞生理状态下，定位检测蛋白-蛋白互作、蛋白表达或蛋白翻译后修饰，检测结果更精确、真实、可靠。
Duolink实验——利用PLA技术检测Smad复合体
BMP4_Smad信号通路具有非常重要的作用，研究表明BMP4通过Smad1/5/8信号通路可促进哺乳动物卵原干细胞分化。该课程详细讲述了通过原位PLA技术可检测到BMP4信号转导下游效应因子Smad1/5/8与Smad4形成复合体，进而激活一系列基因的转导。
Cell and chemical biology of mitosis