微芯片上的生化室
要阻止撒哈拉南部非洲各国的疾病流行，仅靠药品是不够的。我们还需要与之配套的诊断工具。TED资深会员Frederick Balagadde向我们展示如何通过微型化技术把笨重昂贵的化验室集成至一个芯片大小，从而大大提高它的诊断能力和普及性。
Frederick Balagadde:微芯片上的生化室
要阻止撒哈拉南部非洲各国的疾病流行，仅靠药品是不够的。我们还需要与之配套的诊断工具。TED资深会员Frederick Balagadde向我们展示如何通过微型化技术把笨重昂贵的化验室集成至一个芯片大小，从而大大提高它的诊断能力和普及性。
囊泡运输的生化重组
Randy Schekman (Berkeley) Part 2: Biochemical Reconstitution of Transport Vesicle Budding
Secretion mutants that block protein exit from the endoplasmic reticulum define genes involved in the formation, targeting and fusion of a small vesicle intermediate. SEC genes corresponding to the mutants defective in vesicle budding define the cytoplasmic machinery responsible for transport vesicle morphogenesis. A biochemical reaction that reproduces ER vesicle budding was reconstituted with gently-broken yeast cells and pure recombinant Sec proteins required in vivo for this budding event. The Sec proteins assemble on the ER membrane in the presence of GTP which activates a small GTPase, Sar1, initiating the formation of a coat protein complex called COPII. See more athttp://www.ibioseminars.org