杜弈奇:益生菌在IBS功能性胃肠病治疗上的研究-第3段
本短片讨论了IBS功能性胃肠病的发病率,使用婴儿双歧杆菌35624、植物乳酸杆菌299v(DSM 9843)的治疗效果,以及益生菌疗效不确定性的研究。
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益生菌在抗生素相关性腹泻(ADD)治疗上的研究、以及粪便微生态移植(FMT)治疗艰难梭菌感染(CDI)的研究介绍。
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在幽门螺旋杆菌感染(HP),消化性溃疡等疾病上,益生菌如何提高治愈率,降低副作用,以及益生菌种类和数量对HP治疗效果的影响。
Western Blot 第1阶段:样品制备
Novus Biologicals Visual Protocols: In phase 1 of the western blot procedure, you will learn how to prepare your samples before loading them into a gel. Here we isolate protein from cultured cells, quantify total protein concentrations with a BCA assay, add loading buffer to the sample, and heat the sample. Additional help can be found in the support section of http://www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.
Western Blot 第2阶段:蛋白电泳(SDS-PAGE)
Novus Biologicals Visual Protocols: In phase 2 of the western blot procedure, you will learn how to load a gel and separate the proteins through electrophoresis, based upon protein weight. Additional help can be found in the support section of http://www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.
Western Blot 第3阶段:膜转移
Novus Biologicals Visual Protocols: In phase 3 of the western blot procedure, you will learn how to transfer your proteins from the SDS-PAGE gel onto a PVDF or nitrocellulose membrane. Here we remove the gel from the cassette, and stack it in a sandwich comprised of a sponge, filter paper, the gel, membrane, filter paper, and sponge. The negatively charged proteins will then transfer onto the membrane toward the positive current. Additional help can be found in the support section of http://www.novusbio.com, through our live chat service, or by calling us directly to talk with our elite customer and technical service scientists.