SynVivo的应用
SynVivo是一款模拟体内血管环境的微流体芯片，可以模拟仓鼠、大鼠和小鼠的不同血管。提供形态和生物学仿真微环境，可以对药物等微粒与细胞的相互作用进行实时观察研究，其中一些特殊芯片可以培养特定组织类型的细胞，如神经元、肝细胞或肿瘤细胞等。
SynVivo在体外条件下，利用微循环网络模拟在体条件下细胞/微粒粘附、细胞-细胞或细胞-微粒相互作用。研究流体和形态对药物研发和细胞研究的影响.。得到剪切力-粘附效应图，分叉与分支粘附效应等。研究人员只需用适当的底物覆盖微芯片的通道（如纤连蛋白），然后在其中培养细胞，就可以建立人工的体内环境。这一系统可通过流动的液体来模拟天然的血液。
SynVivo在细胞行为、药物粒子粘附和吸收机制、药物输送、药物发现、药物毒性分析等领域有广泛应用。
化学糖生物学 - Carolyn Bertozzi P1
本视频由科普中国和生物医学大讲堂出品
Carolyn Bertozzi (UC Berkeley) Part 1: Chemical Glycobiology
Part 1 A large part of an organism's complexity is not encoded by its genome but results from post-translational modification. Glycosylation, or the addition of sugar molecules to a protein is an example of such a modification. These sugars, or glycans, are often complex, branched molecules specific to particular cells. Cell surface glycans determine human blood types, allow viral infections and play a key role in tissue inflammation. See more at http://www.ibioseminars.org
生物糖组成像方法 - Carolyn Bertozzi P2
本视频由科普中国和生物医学大讲堂出品
Carolyn Bertozzi (UC Berkeley) Part 2: Imaging the Glycome
Since glycans cannot be labeled with genetically-encoded reporters such as GFP, bioorthoganal reactions have been developed to allow their labeling and imaging. In this lecture, Bertozzi describes the chemistry and imaging methodology used to view glycoproteins in cells and whole organisms. See more at http://www.ibioseminars.org
控制老化的基因 - Cynthia Kenyon P1
本视频由科普中国和生物医学大讲堂出品
Cynthia Kenyon (UCSF) Part 1: Genes that Control Aging
Once it was thought that aging was just a random and haphazard process. Instead, the rate of aging turns out to be subject to regulation by transcription factors that respond to hormones and other signals. In the nematode C. elegans, in which many key discoveries about aging were first made, the aging process is subject to regulation by food intake, sensory perception, and signals from the reproductive system. Changing genes and cells that affect aging can lengthen lifespan by six fold, and can also delay age-related disease, such as the growth of tumors.
来自生殖系统的信号显示衰老的规律 - Cynthia Kenyon P2
本视频由科普中国和生物医学大讲堂出品
Cynthia Kenyon (UCSF) Part 2: The Regulation of Aging by Signals from the Reproductive System
Once it was thought that aging was just a random and haphazard process. Instead, the rate of aging turns out to be subject to regulation by transcription factors that respond to hormones and other signals. In the nematode C. elegans, in which many key discoveries about aging were first made, the aging process is subject to regulation by food intake, sensory perception, and signals from the reproductive system. Changing genes and cells that affect aging can lengthen lifespan by six fold, and can also delay age-related disease, such as the growth of tumors.
Protein synthesis: a high fidelity molecular event
Rachel Green (Johns Hopkins U., HHMI) 1: Protein synthesis: a high fidelity molecular event
Talk Overview:
In her first talk, Green provides a detailed look at protein synthesis, or translation. Translation is the process by which nucleotides, the “language” of DNA and RNA, are translated into amino acids, the “language” of proteins. Green begins by describing the components needed for translation; mRNA, tRNA, ribosomes, and the initiation, elongation, and termination factors. She then explains the roles of these players in ensuring accuracy during the initiation, elongation, termination and recycling steps of the translation process. By comparing translation in bacteria and eukaryotes, Green explains that it is possible to determine which components and steps are highly conserved and predate the divergence of different kingdoms on the tree of life, and which are more recent adaptations.
Green’s second talk focuses on work from her lab investigating how ribosomes detect defective mRNAs and trigger events leading to the degradation of the bad RNA and the incompletely translated protein product and to the recycling of the ribosome components. Working in yeast and using a number of biochemical and genetic techniques, Green’s lab showed that the protein Dom34 is critical for facilitating ribosome release from the short mRNAs that result from mRNA cleavage. Experiments showed that Dom34-mediated rescue of ribosomes from short mRNAs is an essential process for cell survival in higher eukaryotes.
Speaker Biography:
Rachel Green received her BS in chemistry from the University of Michigan. She then moved to Harvard to pursue her PhD in the lab of Jack Szostak where she worked on designing catalytic RNA molecules and investigating their implications for the evolution of life. As a post-doctoral fellow at the University of California, Santa Cruz, Green began to study how the ribosome translates mRNA to protein with such accuracy.
Currently, Green is a Professor of Molecular Biology and Genetics at the Johns Hopkins School of Medicine and an Investigator of the Howard Hughes Medical Institute. Research in her lab continues to focus on the ribosome and factors involved in the fidelity of eukaryotic and prokaryotic translation.
Green is the recipient of a Johns Hopkins University School of Medicine Graduate Teaching Award as well as the recipient for numerous awards for her research. She was elected to the National Academy of Sciences in 2012.
Protein synthesis: mRNA surveillance by the ribosome
Rachel Green (Johns Hopkins U., HHMI) 2: Protein synthesis: mRNA surveillance by the ribosome
Talk Overview:
In her first talk, Green provides a detailed look at protein synthesis, or translation. Translation is the process by which nucleotides, the “language” of DNA and RNA, are translated into amino acids, the “language” of proteins. Green begins by describing the components needed for translation; mRNA, tRNA, ribosomes, and the initiation, elongation, and termination factors. She then explains the roles of these players in ensuring accuracy during the initiation, elongation, termination and recycling steps of the translation process. By comparing translation in bacteria and eukaryotes, Green explains that it is possible to determine which components and steps are highly conserved and predate the divergence of different kingdoms on the tree of life, and which are more recent adaptations.
Green’s second talk focuses on work from her lab investigating how ribosomes detect defective mRNAs and trigger events leading to the degradation of the bad RNA and the incompletely translated protein product and to the recycling of the ribosome components. Working in yeast and using a number of biochemical and genetic techniques, Green’s lab showed that the protein Dom34 is critical for facilitating ribosome release from the short mRNAs that result from mRNA cleavage. Experiments showed that Dom34-mediated rescue of ribosomes from short mRNAs is an essential process for cell survival in higher eukaryotes.
Speaker Biography:
Rachel Green received her BS in chemistry from the University of Michigan. She then moved to Harvard to pursue her PhD in the lab of Jack Szostak where she worked on designing catalytic RNA molecules and investigating their implications for the evolution of life. As a post-doctoral fellow at the University of California, Santa Cruz, Green began to study how the ribosome translates mRNA to protein with such accuracy.
Currently, Green is a Professor of Molecular Biology and Genetics at the Johns Hopkins School of Medicine and an Investigator of the Howard Hughes Medical Institute. Research in her lab continues to focus on the ribosome and factors involved in the fidelity of eukaryotic and prokaryotic translation.
Green is the recipient of a Johns Hopkins University School of Medicine Graduate Teaching Award as well as the recipient for numerous awards for her research. She was elected to the National Academy of Sciences in 2012.
Assembly-Line Biosynthesis of Polyketide Antibiotics:Part 1
Chaitan Khosla从进化生物学、化学、结构研究装配线polyketid抗生素生物合成，介绍装配线的机制，分析一个模块的SAXS和连续两模块SAXS。
Assembly-Line Biosynthesis of Polyketide Antibiotics:Part2
从装配线酶的工具和特异性研究体内的重构，纯化的蛋白质的装配线重建。讲解核心反应分析、 定向性、下游延伸单位的特异性酶、装配线的扩展单元的特异性、反式互补的高活性酶。
Synthetic Biology & Metabolic Engineering Teaching an Old Bacterium New Tricks
在第一部分，普莱瑟博士解释说，合成生物学涉及工程原理应用到生物的系统建立生物机器。在建筑这些机器的关键材料是合成DNA。合成DNA可以添加在不同的组合，以生物宿主，如细菌，把它们变成化学工厂，能生产小分子的选择。