study the pathological features of diseases using induced pluripotent stem cells derived form patient's somatic cells
The limited experimental access to disease-affected human tissues has severely impeded the elucidating of molecular mechanisms underlying disease development. Generation of induced pluripotent stem cells (iPSCs) by over-expression of defined transcription factors in somatic cells, in particular in those from patient somatic cells, presents an attractive and promising approach to model the early stages of diseases in vitro and to screen novel biomarkers as well as therapeutic medicines. Recently, many research groups have independently reported that patient-specific iPSC-derived cells recapitulated multiple features of pathological events of a particular disease, offering experimental evidence of utilizing patient-specific iPSCs to model diseases and reevaluate the current therapies. We have derived iPSC lines using somatic cells of patients suffering from Klinefelter's Syndrome (KS) and Alzheimer's Disease (AD) and explored the possibility to use these iPSC lines to recapitulate the pathological features of the diseases. Our results show that patient's specific iPSC lines provide good opportunity to study the development and treatment of diseases.
sRNA Induces the Large-scale Transdetermination of Mesenchymal stem Cells into Hematopoietic stem Cells in Human.
Mesenchymal stem cells (MSCs) can differentiate into cells of bone, endothelium, adipose tissue, cartilage, muscle, and brain. However, whether they can transdeterminate into hematopoietic stem cells (HSCs) remains unsolved. We report here that a subpopulation of human MSCs that are CD44+,CD29+, CD105+, CD166+,CD133-,CD34- could differentiate into hematopoietic stem cells (CD150+/CD133+/CD34+) and their descending blood cells in vitro, when transfected with new endogenous shRNAs The sRNA was high-effectively delivered into MSCs by a novel peptide means. These induced MSC-HSCs could form different types of hematopoietic colonies as nature-occurring HSCs did. Upon transplantation into sublethally irradiated NOD/SCID mice, these MSC-HSCs engrafted and differentiated into all hematopoietic lineages such as erythrocytes, lymphocytes, myelocytes and thrombocyte. More importantly, these induced HSCs could successfully engraft and effectively function in patients with severe aplastic anemia. Furthermore, we demonstrated the first evidence that the transdetermination of MSCs was induced by acetylation of histone proteins and activation of many transcriptional factors. Together, our findings identify the sRNAs that dictates a directed differentiation of MSCs toward HSCs and open up a new source for HSCs used for the treatment of blood diseases and artificial stem cell-made blood.
GE公司His标签蛋白纯化预装柱Histrap™ FF Crude使用技巧
主要介绍了His标签蛋白纯化预装柱Histrap™ FF Crude的实验过程，原理，说明，一般事项，样品制备，纯化操作， 按比例放大， 柱的清洗及保存等。
immunotherapy against cancers(position of sellular therapy)
study the pathological features of diseases using induced pluripotent stem cells derived form patient's somatic cells
The limited experimental access to disease-affected human tissues has severely impeded the elucidating of molecular mechanisms underlying disease development. Generation of induced pluripotent stem cells (iPSCs) by over-expression of defined transcription factors in somatic cells, in particular in those from patient somatic cells, presents an attractive and promising approach to model the early stages of diseases in vitro and to screen novel biomarkers as well as therapeutic medicines. Recently, many research groups have independently reported that patient-specific iPSC-derived cells recapitulated multiple features of pathological events of a particular disease, offering experimental evidence of utilizing patient-specific iPSCs to model diseases and reevaluate the current therapies. We have derived iPSC lines using somatic cells of patients suffering from Klinefelter's Syndrome (KS) and Alzheimer's Disease (AD) and explored the possibility to use these iPSC lines to recapitulate the pathological features of the diseases. Our results show that patient's specific iPSC lines provide good opportunity to study the development and treatment of diseases.
免疫印迹（Western Blot）操作流程
Cst科学家所使用的用于验证抗体的详细的Western Blot操作步骤和注意事项，从样本制备直至检测显影。详细展示了关键操作步骤的改变将对实验结果造成不同程度的影响，因此，遵循Cst经验证的操作步骤非常重要。
免疫印迹（Western Blot）的问题诊断和解决对策
针对Western Blot的常见问题，给出了一些发现问题的小技巧和相应的解决方案，以保证您可以在最短的时间内得到期望的结果。
一支Cst 最佳抗体是如何炼成的？
以一个兔单克隆抗体 Vimentin (D21H3) XP® Rabbit mAb #5741“千里挑一”的故事为例，讲述Cst 抗体的构思、开发、生产和在生物医学研究领域的实际应用。
stefan Larsson:医生们可以互相学习
不同医院的不同手术有着不同的结果。但是病人不知道数据, 所以使得选外科医生成为了一个高风险的猜测游戏。
史帝芬·拉森（stefan Larsson）研究了当医生开始衡量并分享他们的髋关节手术的结果时（比如说什么是最有效的方法）会发生的情况。
如果医生们可以相互学习并形成一个反馈循环，医疗保健会不会变得更好、更便宜？
Ernest Madu向人们展示世界一流的医疗保健服务（1）
Ernest Madu医生在牙买加的金斯敦开了家加勒比海心脏研究所，通过精心的设计，高明的技术选择，秉持着一颗真诚为民服务的心，Ernest Madu向世人证明——发展中国家也能提供世界一流的医疗保健服务。