Simultaneous quantification of 47 gene expression in FFpe samples by a novel PCR-free approach
基因表达(gene expression)是指细胞在生命过程中，把储存在DNA顺序中遗传信息经过转录和翻译，转变成具有生物活性的蛋白质分子。生物体内的各种功能蛋白质和酶都是同相应的结构基因编码的。差别基因表达(differential gene expression)指细胞分化过程中，奢侈基因按一定顺序表达，表达的基因数约占基因总数的5%～10%。
Erich Gnaiger：Life Style and Mitochondrial Competence – Modern Drugs for T2 Diabetes in Aging and Degenerative Diseases.
D. Swarovski Research Laboratory (Mitochondrial Physiology), Dept. General, Visceral and Transplant Surgery, Innsbruck Medical University; and OROBOROS INSTRUMENTS, Innsbruck, Austria. - Email: erich.gnaiger@oroboros.at
The contribution of mitochondrial dysfunction to the etiology of T2 diabetes and a range of preventable metabolic diseases is the subject of intensive current research with world-wide health implications.
Recently these investigations gained depth and scope by technological advances for diagnosis of mitochondrial function by comprehensive OXPHOS analysis using high-resolution respirometry [1,2]. Fundamental questions of a causal relationship, however, between compromised mitochondrial function and development of T2 diabetes remain to be resolved [3,4] to optimize prevention and treatment of insulin resistance.
For preventable diseases such as T2 diabetes, the evolutionary background of mitochondrial competence provides a solid basis for improved and broad application of a well established modern drug, mtLSD.
Post-industrial societies are characterized by a high-energy input lifestyle with diminished physical activity and high incidence of non-transmittable diseases, in comparison to human populations where physical work is essentially important for sustaining life and in which degenerative diseases (T2 diabetes, various cancers, Alzheimer's) are essentially absent [5]. The capacity of oxidative phosphorylation (OXPHOS) is increased or maintained high by a life style involving endurance exercise and strength training [6].
Life style changes from the age of 20-30 years to the elderly, but is subject to change and intervention. Depending on group selection in cross-sectional studies, OXPHOS capacity declines from the age of 20-30 years [7,8], or is independent of age up to 80 years [9,10].
Independent of age, there is a strong decline of OXPHOS capacity in human vastus lateralis from BMI of 20 to 30 [1]. At a BMI >30, a threshold OXPHOS capacity is reached in human v. lateralis that may be characteristic of a low-grade inflammatory state (‘mitochondrial fever’).
Onset of degenerative diseases (T2 diabetes, neuromuscular degeneration, various cancers) and mitochondrial dysfunction interact in an amplification loop progressing slowly with age, such that cause and effect of mitochondrial dysfunction cannot be distinguished. Diminished antioxidant capacity at low mitochondrial density is an important mechanistic candidate in the state of mitochondrial fever.
For implementing a life style supporting mitochondrial competence and preventing degenerative diseases in modern societies, we need (1) extended research programmes focused on the causative link between mitochondrial competence and effective prevention of degenerative diseases, (2) educational programmes on mitochondrial physiology targeted at general practitioners, teachers and the society at large, (3) cooperation of health care and insurance organizations to support preventive life style activities, and (4) do not miss any opportunity in taking the lead in living the mtLife Style Drug (mtLSD).
ACD RNAscope®原位定量技术在精准医疗研究中的应用
RNAscope®原位定量专利技术由美国新兴的分子病理领导者ACD公司(Advanced Cell Diagnostics, Inc., California, USA)开发，通过专利的双“Z”探针设计，使RNA原位杂交具有高度特异性、单分子检测的敏感性并有极高的信噪比，能够在单细胞水平同时定量多个RNA的表达，在获得单细胞中单拷贝RNA表达数据的同时提供完整的组织形态学信息，提高对疾病与标志物之间复杂的生物学相关性的认识，是理想的能够用于NGS和芯片技术后期转化研究技术平台。
自2011年技术推广以来,其应用已在如Nature、Science、NEJM等国际顶级期刊发表超过300篇SCI论文。研究涵盖了感染及免疫、肿瘤、神经生物学、干细胞及发育、非编码RNA、表观遗传学等基础医学领域，以及靶标鉴别和验证、临床前安全性评价和药效评估等药物开发研究。
本课程详细介绍RNAscope®技术的基本原理，技术特点以及在肿瘤、非编码RNA、病毒及免疫等研究领域的应用实例。由于课程内容全英文，更多中文资料与信息请访问ACD中国官方微信号（ACD_China）或发送邮件info_china@acdbio.com咨询。
Western Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel System(Aubin penna.Ph.D)
Western Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel System(Aubin penna.Ph.D)
RNAscope®技术原理介绍
RNAscope®®原位定量专利技术由美国新兴的分子病理领导者ACD公司(Advanced Cell Diagnostics, Inc., California, USA)开发，通过专利的双“Z”探针设计，使RNA原位杂交具有高度特异性、单分子检测的敏感性并有极高的信噪比，能够在单细胞水平同时定量多个RNA的表达，在获得单细胞中单拷贝RNA表达数据的同时提供完整的组织形态学信息，提高对疾病与标志物之间复杂的生物学相关性的认识，是理想的能够用于NGS和芯片技术后期转化研究技术平台。
自2011年技术推广以来,其应用已在如Nature、Science、NEJM等国际顶级期刊发表超过300篇SCI论文。研究涵盖了感染及免疫、肿瘤、神经生物学、干细胞及发育、非编码RNA、表观遗传学等基础医学领域，以及靶标鉴别和验证、临床前安全性评价和药效评估等药物开发研究。
请访问ACD官网www.acdbio.com。更多中文资料与信息请访问中国官方微信号（ACD_China）咨询。
将RNAscope®技术应用于病毒学的研究
Advanced Cell Diagnostics邀请了美国加利福尼亚大学（Davis）微生物、免疫及病理学系教授Patricia pesavento，为大家介绍应用RNAscope®技术在研究新发现的病毒及致病病因、病理方面的研究。引起疾病的病毒可以跨越种族感染，通过测序发现新型病毒，利用RNAscope®进行病毒病理学分析，以准确判断疾病及复杂的宿主病毒相互影响。在这一讲座中，pesavento教授以乳头瘤病毒等举例，对病毒的潜伏、致瘤和急性裂解细胞分析，分享了病毒学的研究进展。
RNAscope®技术可以针对任一病毒（或亚型）序列设计特异性探针，通过原位定量检测获得病毒感染、潜伏及与宿主反应的研究信息。详细信息请访问ACD官网www.acdbio.com。更多中文资料请关注中国官方微信号（ACD_China）咨询。
如何成功的开展RNAscope®实验：操作步骤指南
该视频由美国Advanced Cell Diagnostics公司的高级科学家讲解如何开展RNAscope®实验。内容包括：RNAscope®技术原理、实验所需的准备工作、实验过程中的注意事项和技巧、常见问题解答。
RNAscope®®原位定量专利技术由美国ACD公司(Advanced Cell Diagnostics, Inc., California, USA)开发，通过专利的双“Z”探针设计，使RNA原位杂交具有高度特异性、RNA单分子检测的敏感性并兼容自动化高通量分析，能够在单细胞水平同时定量多个RNA的表达。该视频由ACD公司制作，详细信息请访问ACD官网www.acdbio.com。更多中文资料请关注中国官方微信号（ACD_China）咨询。
如何在斑马鱼完整胚胎样本中使用RNAscope®技术进行研究
整胚原位杂交是在完整胚胎和组织中研究基因时空表达模式的有力工具。但现有的实验方法无法准确的直接检测RNA，而且操作耗时，结果和蛋白表达水平不一致。新一代原位定量杂交技术RNAscope®可以在斑马鱼整胚上实现快速高效、精准定量、特异性的多重RNA原位检测。该webinar由美国ACD公司(Advanced Cell Diagnostics, Inc., California, USA)邀请德国Münster大学细胞生物学研究所Erez Raz教授实验室两位研究员Azadeh Paksa 和 Theresa Gros介绍他们利用RNAscope®技术实现斑马鱼整胚上同时原位检测3个RNA分子进行三维荧光分析。详细介绍了实验操作过程，如何优化条件，降低信噪比以及RNAscope®技术相比传统原位杂交技术的绝对优势。
ACD公司提供RNAscope®原位定量杂交专利技术和产品，详细信息请访问ACD官网www.acdbio.com。更多中文资料请关注中国官方微信号（ACD_China）咨询。
文章题目: Simultaneous high-resolution detection of multiple transcripts combined with localization of proteins in whole-mount embryos. Gross-Thebing T, Paksa A, Raz E. BMC Biol. 2014 Aug 15;12(1):55.
RNAscope原位杂交技术助力新冠病毒检测及初步结果展示
RNAscope原位杂交技术助理新冠病毒检测及初步结果展示 1. RNAscope技术原理介绍 2. 在Zika，MERs-Cov，HIV等病毒研究中的应用实例 3. RNAscope应对新冠病毒研究的相关产品及结果展示