sRNA Induces the Large-scale Transdetermination of Mesenchymal Stem Cells into Hematopoietic Stem Cells in Human.
Mesenchymal stem cells (MSCs) can differentiate into cells of bone, endothelium, adipose tissue, cartilage, muscle, and brain. However, whether they can transdeterminate into hematopoietic stem cells (HSCs) remains unsolved. We report here that a subpopulation of human MSCs that are CD44+,CD29+, CD105+, CD166+,CD133-,CD34- could differentiate into hematopoietic stem cells (CD150+/CD133+/CD34+) and their descending blood cells in vitro, when transfected with new endogenous shRNAs The sRNA was high-effectively delivered into MSCs by a novel peptide means. These induced MSC-HSCs could form different types of hematopoietic colonies as nature-occurring HSCs did. Upon transplantation into sublethally irradiated NOD/SCID mice, these MSC-HSCs engrafted and differentiated into all hematopoietic lineages such as erythrocytes, lymphocytes, myelocytes and thrombocyte. More importantly, these induced HSCs could successfully engraft and effectively function in patients with severe aplastic anemia. Furthermore, we demonstrated the first evidence that the transdetermination of MSCs was induced by acetylation of histone proteins and activation of many transcriptional factors. Together, our findings identify the sRNAs that dictates a directed differentiation of MSCs toward HSCs and open up a new source for HSCs used for the treatment of blood diseases and artificial stem cell-made blood.
immunotherapy against cancers(position of sellular therapy)
Interplays between Mesenchymal Stem Cells and Immune Responses
Mesenchymal stem cells (MSCs) exist in almost all tissues and are crucial in maintaining the cellular homeostasis of multicellular organisms. They provide the ultimate cell source for tissue repair and regeneration. Under pathological conditions, these cells are awakened, activated, and mobilized to damaged tissue sites. Since tissue damages are often accompanied by inflammatory factors, from both innate immune response and adaptive immune response, it is sensible that MSCs delicately interact with inflammatory factors at the sites of tissue damages.
Depending on the type and persistence of the inflammatory factors, the activated MSCs could lead either to complete or partial tissue repair, or to chronic inflammation and further tissue damage, such as cancer and fibrosis. Indeed, recent studies have shown that there is a bidirectional interaction between MSCs and inflammatory cells and cytokines. However, much information of this information remains to be elucidated. Further investigations in this newly emerging exciting research area will undoubtedly lead to better understanding of pathogenesis of various diseases and novel treatment strategies.
Discovery: Innovations with Ed Begley Jr
美国著名的《Discovery》频道创新系列节目《Innovations with Ed Begley Jr》全球播出了艾森生物最新研发的新一代实时心肌功能评价系统（xCELLigence RTCA CardioECR）。
本期节目主要关注在医疗健康领域的突破性进展。节目用七分多钟时间报道了艾森生物实时心肌细胞检测这项突破性的技术发明，并通过目前用药安全方面的问题调研及业内知名专家的采访，阐明了该发明在药物早期心脏毒性评价、用药安全、高通量新药筛选、心脏基础研究方面的价值和意义。
Sigma® Life Science——Where bio begins
生命是一个不断扩大、不断进化的宇宙，充满着发现问题和探索未知的机会。每天，突破性的想法重新调整我们的认识，不断打破我们对生物学的认知边界。Sigma® Life Science正在改变世人关于生物学的思维方式，让研究者以独特的视角去探索科学，鼓舞他们去不懈挑战，这里是生物学开始的地方。
Generating B-lymphoblastoid cell lines using Epstein Barr virus transformation.
Generating immortalized B-lymphoblastoid cell lines via Epstein Barr virus transformation using the B95-8 EBV-infected and producing marmoset cell line.
Controlling the Cell Cycle: Anaphase Onset - David O. Morgan
本视频由科普中国和生物医学大讲堂出品
David O. Morgan (UCSF) Part 3: Controlling the Cell Cycle: Anaphase Onset
In the anaphase stage of the cell cycle, the duplicated chromosomes are pulled apart by a machine called the mitotic spindle, resulting in the distribution of a complete set of chromosomes to each of the daughter cells. In the third part of this lecture, I describe the combination of biochemistry and microscopy in my laboratory that led to the discovery of a regulatory switch that triggers the abrupt and synchronous separation of the chromosomes at the onset of anaphase.
Photoreceptors and Image Processing Part 1A - Jeremy Nathans
本视频由科普中国和生物医学大讲堂出品
Jeremy Nathans (Johns Hopkins) Part 1A: Photoreceptors and Image Processing
In this set of lectures, Jeremy Nathans explores the molecular mechanisms within the retina that mediate the first steps in vision. The first lecture focuses on the structure of the light sensing receptors, the intracellular signals that are triggered by light absorption, and the ways in which the retina extracts information from a complex scene. See more at http://www.ibioseminars.org
Photoreceptors and Image Processing Part 1B - Jeremy Nathans
本视频由科普中国和生物医学大讲堂出品
Jeremy Nathans (Johns Hopkins) Part 1B: Photoreceptors and Image Processing
In this set of lectures, Jeremy Nathans explores the molecular mechanisms within the retina that mediate the first steps in vision. The first lecture focuses on the structure of the light sensing receptors, the intracellular signals that are triggered by light absorption, and the ways in which the retina extracts information from a complex scene. See more at http://www.ibioseminars.org