sRNA Induces the Large-scale Transdetermination of Mesenchymal Stem Cells into Hematopoietic Stem Cells in Human.
Mesenchymal stem cells (MSCs) can differentiate into cells of bone, endothelium, adipose tissue, cartilage, muscle, and brain. However, whether they can transdeterminate into hematopoietic stem cells (HSCs) remains unsolved. We report here that a subpopulation of human MSCs that are CD44+,CD29+, CD105+, CD166+,CD133-,CD34- could differentiate into hematopoietic stem cells (CD150+/CD133+/CD34+) and their descending blood cells in vitro, when transfected with new endogenous shRNAs The sRNA was high-effectively delivered into MSCs by a novel peptide means. These induced MSC-HSCs could form different types of hematopoietic colonies as nature-occurring HSCs did. Upon transplantation into sublethally irradiated NOD/SCID mice, these MSC-HSCs engrafted and differentiated into all hematopoietic lineages such as erythrocytes, lymphocytes, myelocytes and thrombocyte. More importantly, these induced HSCs could successfully engraft and effectively function in patients with severe aplastic anemia. Furthermore, we demonstrated the first evidence that the transdetermination of MSCs was induced by acetylation of histone proteins and activation of many transcriptional factors. Together, our findings identify the sRNAs that dictates a directed differentiation of MSCs toward HSCs and open up a new source for HSCs used for the treatment of blood diseases and artificial stem cell-made blood.
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Chaperone-assisted protein folding
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许俊泉：CelSee-新一代CTC平台助力临床与科研
介绍了一些肿瘤治疗相关方面的数据，演讲内容主要包括ＣｌＳｅｅ－新一代ＣＴＣ平台。ＣＴＣ项目合作方案。产品发展展望。博奥晶典介绍。
黄志伟：CRISPR-Cpf1识别CRISPR RNA （crRNA）以及Cpf1剪切pre-crRNA成熟的分子机制
从结构生物学的角度讲解了CRISPR-Cpf1识别CRISPR RNA （crRNA）以及Cpf1剪切pre-crRNA成熟的分子机制。发现Cpf1并不是之前人们推测的二聚体状态，而是一个呈三角形的单体，位于该结构中间是一个带有正电荷的凹槽
Livecyte-定量非标记活细胞成像及分析系统
Livecyte是一款长时间非标记活细胞分析系统，其独特的QPI技术使得在细胞实验中不仅可以研究细胞群体的变化，也可以研究其中单个细胞的动力学和形态学变化，得到比其他实验方法更精确的结论。传统的划痕实验通常使用终点法，通过设置不同实验组来间接监测划痕愈合的过程。这种方式引入了不同实验组带来的实验误差，同时荧光的引入也对细胞生长状态带来了干扰。在观测细胞群体的情况下，通过这些手段我们往往还是不能很清楚的了解到划痕的愈合是由于药物增加了细胞增殖还是细胞迁移，并且一些低浓度药物对细胞的影响也不能确定。英国Phasefocus公司携手英国Hull大学(Univerisity of Hull)利用Liveycyte研究了在原代成纤维细胞的划痕实验中，划痕愈合的速度，细胞的运动轨迹，迁移速度，数量和细胞本身面积的变化，从而得出细胞在愈合过程中已经开始分化的结论。