Study the pathological featurES of diseasES using induced pluripotent stem cells derived form patient's somatic cells
The limited experimental accESs to disease-affected human tissuES has severely impeded the elucidating of molecular mechanisms underlying disease development. Generation of induced pluripotent stem cells (iPSCs) by over-exprESsion of defined transcription factors in somatic cells, in particular in those from patient somatic cells, prESents an attractive and promising approach to model the early stagES of diseasES in vitro and to screen novel biomarkers as well as therapeutic medicinES. Recently, many rESearch groups have independently reported that patient-specific iPSC-derived cells recapitulated multiple featurES of pathological events of a particular disease, offering experimental evidence of utilizing patient-specific iPSCs to model diseasES and reevaluate the current therapiES. We have derived iPSC linES using somatic cells of patients suffering from Klinefelter's Syndrome (KS) and Alzheimer's Disease (AD) and explored the possibility to use thESe iPSC linES to recapitulate the pathological featurES of the diseasES. Our rESults show that patient's specific iPSC linES provide good opportunity to study the development and treatment of diseasES.
sRNA InducES the Large-scale Transdetermination of MESenchymal Stem Cells into Hematopoietic Stem Cells in Human.
MESenchymal stem cells (MSCs) can differentiate into cells of bone, endothelium, adipose tissue, cartilage, muscle, and brain. However, whether they can transdeterminate into hematopoietic stem cells (HSCs) remains unsolved. We report here that a subpopulation of human MSCs that are CD44+,CD29+, CD105+, CD166+,CD133-,CD34- could differentiate into hematopoietic stem cells (CD150+/CD133+/CD34+) and their dEScending blood cells in vitro, when transfected with new endogenous shRNAs The sRNA was high-effectively delivered into MSCs by a novel peptide means. ThESe induced MSC-HSCs could form different typES of hematopoietic coloniES as nature-occurring HSCs did. Upon transplantation into sublethally irradiated NOD/SCID mice, thESe MSC-HSCs engrafted and differentiated into all hematopoietic lineagES such as erythrocytES, lymphocytES, myelocytES and thrombocyte. More importantly, thESe induced HSCs could succESsfully engraft and effectively function in patients with severe aplastic anemia. Furthermore, we demonstrated the first evidence that the transdetermination of MSCs was induced by acetylation of histone proteins and activation of many transcriptional factors. Together, our findings identify the sRNAs that dictatES a directed differentiation of MSCs toward HSCs and open up a new source for HSCs used for the treatment of blood diseasES and artificial stem cell-made blood.
Study the pathological featurES of diseasES using induced pluripotent stem cells derived form patient's somatic cells
The limited experimental accESs to disease-affected human tissuES has severely impeded the elucidating of molecular mechanisms underlying disease development. Generation of induced pluripotent stem cells (iPSCs) by over-exprESsion of defined transcription factors in somatic cells, in particular in those from patient somatic cells, prESents an attractive and promising approach to model the early stagES of diseasES in vitro and to screen novel biomarkers as well as therapeutic medicinES. Recently, many rESearch groups have independently reported that patient-specific iPSC-derived cells recapitulated multiple featurES of pathological events of a particular disease, offering experimental evidence of utilizing patient-specific iPSCs to model diseasES and reevaluate the current therapiES. We have derived iPSC linES using somatic cells of patients suffering from Klinefelter's Syndrome (KS) and Alzheimer's Disease (AD) and explored the possibility to use thESe iPSC linES to recapitulate the pathological featurES of the diseasES. Our rESults show that patient's specific iPSC linES provide good opportunity to study the development and treatment of diseasES.
JSR Life SciencES诊断试剂原料
诊断试剂原料类产品包括：诊断试剂用原料（乳胶微球、磁性粒子、封闭剂）、研究用材料试剂（超常磁珠、粒子和功能配体）、和标准离子。主要用于诊断试剂的研发与生产、蛋白质与核酸的分离、微粒子计数器的校正等。
JSR Life SciencES业务介绍
捷时雅（上海）商贸有限公司是JSR 集团在中国的全资子公司，其生物技术部全面负责JSR Life SciencES公司及其控股子公司的生物工艺器材、诊断试剂原料和医用高分子材料等产品在中国的市场推广、销售和技术支持工作。生物工艺器材类产品包括：亲和层析填料（protein A 亲和填料）、离子交换层析膜（NatriFlo HD-Q)、三维细胞培养耗材及试剂（SCIVAX)等，主要用于新药候选物筛选、药物毒性评估、生物医药工业的下游分离和纯化及大规模制备生产等领域；
JSR Life SciencES生物工艺器材
JSR Life SciencES给中国的抗体药物研发及生产单位，提供一套独有的下游工艺解决方案。
Amspere JWT203 Protein A 填料用于抗体药物的捕获，NatriFlo HD-Q 离子交换膜用于抗体药物精细 纯化步骤，有效降低用户的生产成本，缩短工艺时间，提高工艺的稳定性。ChromaCon 的连续层析系统为抗体药物的质控分析提供高精度高分辨率的工具，有效分离和分析抗体酸碱变体。
免疫印迹（WEStern Blot）操作流程
CST科学家所使用的用于验证抗体的详细的WEStern Blot操作步骤和注意事项，从样本制备直至检测显影。详细展示了关键操作步骤的改变将对实验结果造成不同程度的影响，因此，遵循CST经验证的操作步骤非常重要。
免疫印迹（WEStern Blot）的问题诊断和解决对策
针对WEStern Blot的常见问题，给出了一些发现问题的小技巧和相应的解决方案，以保证您可以在最短的时间内得到期望的结果。
AndrES Lozano帕金森氏综合症、抑郁症和关闭它们的开关
大脑深部电击术变得相当精准。借由这项科技，外科医生几乎能将电极置入大脑的任何区域,像电台或恒温器的调节旋钮一项,增强或减弱回路信号。一位罹患帕金森氏综合症的妇女，在电击后立即停止肢体抖动，以及老年痴呆症患者脑部功能恢复，我们看到这项科技带来的巨大改变。
ErnESt Madu向人们展示世界一流的医疗保健服务（1）
ErnESt Madu医生在牙买加的金斯敦开了家加勒比海心脏研究所，通过精心的设计，高明的技术选择，秉持着一颗真诚为民服务的心，ErnESt Madu向世人证明——发展中国家也能提供世界一流的医疗保健服务。