Cell and chEMical biology of mitosis
纳米生物效应与安全性实验室在2004年首次发现内含Gd原子的金属富勒烯三明治纳米结构颗粒可以直接作为肿瘤的高效低毒化疗药物以来，已经从分子免疫、神经调控、干细胞分化、血管生成等诸多方面对纳米颗粒直接作为高效低毒化疗药物的药效和机制，进行了长达6年多的研究，在国际学术刊物上连续发表了一系列的研究成果，逐渐形成较大的国际影响力。
金颖：Fox3 suppresses NFAT-mediated differentiation to maintain self-renewal of EMbryonic stEM cells
金颖教授为分子发育生物学研究室主任，健康科学中心研究员。金教授介绍了Fox3通过抑制NFAT介导的分化维持了胚胎干细胞的自我更新的机制等前沿发现。
Pluripotency-associated transcription factor Foxd3 is required for maintaining pluripotent cells. However, molecular mechanisms underlying its function are largely unknown.
Here, we report that Foxd3 suppresses differentiation induced by Calcineurin-NFAT signaling to maintain the ESC identity. Mechanistically, Foxd3 interacts with NFAT proteins and recruits co-repressor Tle4, a mEMber of the Tle suppressor family highly expressed in undifferentiated ESCs, to repress NFATc3’s transcriptional activities.
Furthermore, global transcriptome analysis shows that Foxd3 and NFATc3 co-regulate a set of differentiation-associated genes in ESCs. Collectively, our study establishes a molecular and functional link between a pluripotency-associated factor and an important ESC differentiation-inducing pathway.
Western Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel SystEM(Aubin Penna.Ph.D)
Western Blot Using The invitrogen NuPAGE Novex Bis-Tris MiniGel SystEM(Aubin Penna.Ph.D)
Photoreceptors and Image Processing Part 1A - JerEMy Nathans
本视频由科普中国和生物医学大讲堂出品
JerEMy Nathans (Johns Hopkins) Part 1A: Photoreceptors and Image Processing
In this set of lectures, JerEMy Nathans explores the molecular mechanisms within the retina that mediate the first steps in vision. The first lecture focuses on the structure of the light sensing receptors, the intracellular signals that are triggered by light absorption, and the ways in which the retina extracts information from a complex scene. See more at http://www.ibiosEMinars.org
Photoreceptors and Image Processing Part 1B - JerEMy Nathans
本视频由科普中国和生物医学大讲堂出品
JerEMy Nathans (Johns Hopkins) Part 1B: Photoreceptors and Image Processing
In this set of lectures, JerEMy Nathans explores the molecular mechanisms within the retina that mediate the first steps in vision. The first lecture focuses on the structure of the light sensing receptors, the intracellular signals that are triggered by light absorption, and the ways in which the retina extracts information from a complex scene. See more at http://www.ibiosEMinars.org
Stability of Morphogen Gradients & MovEMent of Molecules
In my second lecture I describe experiments using EGFP tagged Bicoid to follow Bcd gradient establishment in living EMbryos, and to test various aspects of the simple model. Despite continuous synthesis of new Bcd protein at the anterior end of the egg, we find that the concentration of Bcd in nuclei at any given point along the anterior posterior axis is constant over time and is reproducible from EMbryo to the next. This reproducibility means that the gradient is sufficiently robust to provide positional information and thus can accurately direct gene activities. One the other hand, quantitative imaging experiments point to several features of the gradient that are hard to explain - how target genes activated by Bcd distinguish relatively subtle differences in low concentrations, and how Bcd molecules move from the anterior site of their synthesis to the site of their transcriptional activity. See more at http://www.ibiosEMinars.org
Introducing the Opera Phenix™ High Content Screening SystEM
Introducing Opera Phenix™, a next-generation, confocal, high content screening systEM designed for high-throughput, phenotypic screening and assays using complex disease models, such as primary cells and microtissues. Find out more at http://bit.ly/1eZM4Ok.
AssEMbly-Line Biosynthesis of Polyketide Antibiotics:Part 1
Chaitan Khosla从进化生物学、化学、结构研究装配线polyketid抗生素生物合成，介绍装配线的机制，分析一个模块的SAXS和连续两模块SAXS。
AssEMbly-Line Biosynthesis of Polyketide Antibiotics:Part2
从装配线酶的工具和特异性研究体内的重构，纯化的蛋白质的装配线重建。讲解核心反应分析、 定向性、下游延伸单位的特异性酶、装配线的扩展单元的特异性、反式互补的高活性酶。
Rescuing US bIomedical Research From Its SystEMic Flaws
这些在生物医学研究领域的领导者已经把他们的注意力转向问题这是面临的实践和可持续发展的基础研究美国的企业。他们的关切和想法应对这些挑战已发表在诉讼程序中的美国国家科学院（2014年4月22日）。在谈话中，Alberts，Kirschner，蒂尔曼和斯讨论本文的成因，需要关注的核心问题，以及需要涉及更大的科学社区在保障活力的过程中生物医学研究企业。