《免疫学杂志》发布2018年下载量**的20篇文章

《免疫学杂志》（Journal of Immunology）近日发布了“Top Downloads for 2018”目录。质谱流式技术作为免疫学研究中越来越不可或缺的方法，共有3篇文章名列其中，且听小编为您一一介绍。

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文章可说是质谱流式初学者的福音，对数据分析和可视化进行了基础、详尽的描述。

在质谱流式的数据分析中，基于Cytobank软件和R语言包的viSNE, SPADE, X-shift, PhenoGraph, Citrus等分析方法使用非常广泛，在这篇文章中，作者向广大读者详细介绍了如何使用这些方法对数据进行分析。（下载数量超过4000次）

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另一篇文章中，作者利用质谱流式技术对健康人血液中的Th和Treg进行了精细的分群研究。通过同时对CD3+CD4+ Th, Treg和Tr1细胞表面23个标志物的检测分析发现，除了CD3+CD4+ Th、Treg和Tr1细胞群之外，共有11个细胞、4个Treg和1个Tr1细胞亚群，进一步说明了T细胞的多样性。这一研究为不同亚群在免疫性病变中的异常提供了重要的对照参考数据。（下载数量超过2000次）

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第三篇文章是一篇关于CAR-T的综述。文章详细阐述了CAR-T的原理、当前的研究进展以及挑战。同时指出，CyTOF®作为一项新技术，在灵敏度、系统分析等方面较传统方法都有提升，对全面了解CAR-T细胞疗法在癌症治疗中的免疫反应来说是一种非常准确、高效的方法，是CAR-T治疗效果和耐药性研究的有效工具。

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附录：Top Downloads For 2018

1.Oral NaHCO Activates a Splenic Anti-Inflammatory Pathway: Evidence That Cholinergic Signals Are Transmitted via Mesothelial Cells

Sarah C. Ray, et al., J Immunol 2018 200: 3568-3586

2. Peptide–MHC Class I Tetramers Can Fail To Detect Relevant Functional T Cell Clonotypes and Underestimate Antigen-Reactive T Cell Populations

Cristina Rius, et al., J Immunol 2018 200: 2263-2279

3. Highly Efficient and Versatile Plasmid-Based Gene Editing in Primary T Cells

Mara Kornete, Romina Marone and Lukas T. Jeker, J Immunol 2018 200: 2489-2501

4. A Beginner’s Guide to Analyzing and Visualizing Mass Cytometry Data

Abigail K. Kimball, et al., J Immunol 2018 200: 3-22

5. Functional Anti-TIGIT Antibodies Regulate Development of Autoimmunity and Antitumor Immunity

Karen O. Dixon, et al., J Immunol 2018 200: 3000-3007

6. Chimeric Antigen Receptor T Cell Therapy: Challenges to Bench-to-Bedside Efficacy

Shivani Srivastava and Stanley R. Riddell, J Immunol 2018 200: 459-468

7. Inhibitors of the PD-1 Pathway in Tumor Therapy

Martin W. LaFleur, et al., J Immunol 2018 200: 375-383

8. Myeloid-Derived Suppressor Cells: Immune-Suppressive Cells That Impair Antitumor Immunity and Are Sculpted by Their Environment

Suzanne Ostrand-Rosenberg and Catherine Fenselau, J Immunol 2018 200: 422-431

9. CCR8 Expression Defines Tissue-Resident Memory T Cells in Human Skin

Michelle L. McCully, et al., J Immunol 2018 200: 1639-1650

10. Cutting Edge: Critical Roles for Microbiota-Mediated Regulation of the Immune System in a Prenatal Immune Activation Model of Autism

Catherine R. Lammert, et al., J Immunol 2018 201: 845-850

11. A Believer’s Overview of Cancer Immunosurveillance and Immunotherapy

Olivera J. Finn, J Immunol 2018 200: 385-391

12. Metabolic Barriers to T Cell Function in Tumors

Ayaka Sugiura and Jeffrey C. Rathmell, J Immunol 2018 200: 400-407

13. Dendritic Cell–Based Cancer Vaccines

Patricia M. Santos and Lisa H. Butterfield, J Immunol 2018 200: 443-449

14. Immune Cell Infiltration and Tertiary Lymphoid Structures as Determinants of Antitumor Immunity

Victor H. Engelhard, et al., J Immunol 2018 200: 432-442

15. Discovery of the IL-23/IL-17 Signaling Pathway and the Treatment of Psoriasis

Jason E. Hawkes, et al., J Immunol 2018 201: 1605-1613

16. Hitting the Target: How T Cells Detect and Eliminate Tumors

Anthony E. Zamora, Jeremy Chase Crawford and Paul G. Thomas, J Immunol 2018 200: 392-399

17. Unlocking the Complexities of Tumor-Associated Regulatory T Cells

Jaime L. Chao and Peter A. Savage, J Immunol 2018 200: 415-421

18. Eight-Color Multiplex Immunohistochemistry for Simultaneous Detection of Multiple Immune Checkpoint Molecules within the Tumor Microenvironment

Mark A. J. Gorris, et al., J Immunol 2018 200: 347-354

19. Critical Interactions between Immunogenic Cancer Cell Death, Oncolytic Viruses, and the Immune System Define the Rational Design of Combination Immunotherapies

Jacob P. van Vloten, et al., J Immunol 2018 200: 450-458

20. Identity and Diversity of Human Peripheral Th and T Regulatory Cells Defined by Single-Cell Mass Cytometry

Matthew A. Kunicki, et al., J Immunol 2018 200: 336-346