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J Nutr:一滴血检测维生素B12

来源:生物谷 2014-10-29 10:01

2014年10月29日 讯 /生物谷BIOON/ --近日,来自英属哥伦比亚大学的研究人员开发了一种新方法来检测个体机体是否缺失维生素B12,该方法对任何一个个体都非常敏感,包括新生儿及大面积的普通人群,相关研究刊登于国际杂志Journal of Nutrition上。

文章中,研究者表示,检测维生素B12缺失仅需要一滴血就可以进行,研究者将血液滴在包含滤纸的卡片上过夜使血液成为干点,随后对血液干点的敏感性进行分析来测定机体甲基丙二酸(MMA)的含量,MMA是个体维生素B12水平的指示器。

研究者Yvonne Lamers说道,这种微创方法(仅仅刺破个体的手指取血)可以帮助我们轻而易举地测定个体的维生素B12是否缺失,尤其是对于大量样本来讲非常方面快捷,这种新技术也是首次利用干燥的血斑点来分析其敏感性,足以对一位维生素B12缺失的健康个体进行检测。

这种新型技术的开发简化了偏远地区个体血液样本的收集,其目前正在印度尼西亚的农村广泛使用,而且其具有明显的临床意义,可以加入到新生儿的筛查体系中来帮助改善新生儿的健康。维生素B12的缺失如果没有被及时检测到或及时进行治疗,很容易引发大脑延迟发育,减缓婴儿的学习及消化功能。

最后研究者Hilary Vallance表示,目前我们对这种新方法非常有兴趣而且有信心,这种新型技术可以利用一滴血就实现对个体机体维生素B12的缺失进行敏感性地检测。(生物谷Bioon.com)

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Methylmalonic Acid Quantified in Dried Blood Spots Provides a Precise, Valid, and Stable Measure of Functional Vitamin B-12 Status in Healthy Women1,2,3

Theresa H. Schroder, Teo A. W. Quay, and Yvonne Lamers*

Methylmalonic acid (MMA) is a sensitive and specific functional biomarker of vitamin B-12 status, commonly assessed in plasma or serum. Dried blood spots (DBSs) allow simpler and more cost-efficient blood sampling than plasma. To facilitate convenient testing for vitamin B-12 deficiency in large-scale surveys and in population groups from remote areas, we developed a method for MMA quantification in DBSs and tested its applicability as well as the long-term stability of MMA in DBSs at various temperatures. MMA was extracted from an 8-mm DBS punch with water:methanol (95:5, v:v) and methyl-d3-malonic acid as the internal standard. After sample cleanup by ultrafiltration and hexane extraction, MMA was quantified by using reversed-phase LC-tandem mass spectrometry. Extraction conditions were optimized to maximize the detection signal and achieve DBS extract concentrations above the lowest limit of quantification (signal-to-noise ratio ≥ 10) of 10 nmol/L. Recovery was between 93% and 96%. Intra- and interassay variation (CV%) for DBS MMA was 0.49% and 2.3%, respectively. Calibrators showed linearity (R2 = 0.998) between 10 and 10,000 nmol/L. In 94 healthy women, MMA concentrations in DBS extract (min-max: 10.2–80.5 nmol/L) and plasma (min-max: 68–950 nmol/L) were correlated (ρ = 0.90) (P < 0.001). MMA concentrations in DBSs were stable at room temperature for 1 wk, in the refrigerator for 8 wk, and at −80°C for at least 1 y. This simple and robust method allows quantification of MMA in DBSs of healthy individuals. The linear relation between plasma and DBS MMA suggests that DBS MMA could predict plasma MMA, the current reference indicator for functional vitamin B-12 deficiency. With the advantages of minimally invasive specimen collection and no need for laborious blood processing steps, this method has the potential to be a reliable, convenient, and field-applicable alternative for assessment of vitamin B-12 status.

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