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首页 » PLoS One:上海兽医所miRNA参与抗血吸虫侵染机制研究进展

PLoS One:上海兽医所miRNA参与抗血吸虫侵染机制研究进展

来源:生物谷 2014-02-14 13:35

血吸虫病是最严重的人畜共患疾病之一,在热带和亚热带地区影响超过200百万个生物个体。该疾病由血吸虫属的吸虫侵染宿主导致。据报道,在中国有超过46个哺乳动物物种会被日本血吸虫(Schistosomajaponicum)自然侵染。东方田鼠(Microtusfortis)是在中国流行病区中已知的唯一可以自然防止S.japonicum在其体内发育成熟的哺乳动物。来自中国农业科学院上海兽医研究所林矫矫教授和扬州大学兽医学院陶建平教授领衔的团队从microRNA(miRNA)调控网络的角度对M.fortis抵抗S.japonicum侵染的机制进行了深入探讨,研究成果发表在近期出版的PLoS ONE上。

在此项研究中,研究人员首先采用苏木精伊红染色观察比较了在S. japonicum侵染后M.fortis和小鼠(Musmusculus)的不同组织的病理变化。然后使用μParaflo®微流体miRNA芯片(芯片检测由联川生物完成)鉴定同一组织在S. japonicum侵染前后差异表达的miRNAs,以期找到在这两种不同品种的宿主中miRNA在血吸虫侵染过程中发挥的潜在作用。组织学分析表明,在S. japonicum侵染后M. fortis中发生了比M.musculus更为强烈的炎症反应和病理变化。微阵列芯片分析显示,162个miRNAs在两个物种中均有表达,其中在M. fortis的肝脏,脾脏,和肺中分别有12,32个,和34个miRNAs差异表达。这些差异表达的miRNAs主要参与营养代谢,免疫调节等过程。进一步的分析表明,在S. japonicum侵染后在M. fortis而非M.musculus上触发了一系列重要的信号转导通路。

上述研究成果提供了非允许性血吸虫宿主M. fortis调控机制的新视角,也探索了潜在的miRNA调控网络。这些信息也有助于增进当前对血吸虫发育和宿主-寄生虫相互作用的认识。(生物谷Bioon.com)

生物谷推荐英文摘要:

PLoS One     DOI: 10.1371/journal.pone.0085080    

Differential Expression of microRNAs in the Non-Permissive Schistosome Host Microtusfortis under Schistosome Infection

Hongxiao Han, Jinbiao Peng, Yanhui Han,et al.

The reed vole Microtus fortis is the only mammal known in China in which the growth, development and maturation of schistosomes (Schistosoma japonicum) is prevented. It might be that the anti-schistosomiasis mechanisms of M. fortis associate with microRNA-mediated gene expression, given that the latter has been found to be involved in gene regulation in eukaryotes. In the present study, the difference between pathological changes in tissues of M. fortis and of mice (Mus musculus) post-schistosome infection were observed by using hematoxylin-eosin staining. In addition, microarray technique was applied to identify differentially expressed miRNAs in the same tissues before and post-infection to analyze the potential roles of miRNAs in schistosome infection in these two different types of host. Histological analyses showed that S. japonicum infection in M. fortis resulted in a more intensive inflammatory response and pathological change than in mice. The microarray analysis revealed that 162 miRNAs were expressed in both species, with 12 in liver, 32 in spleen and 34 in lung being differentially expressed in M. fortis. The functions of the differentially expressed miRNAs were mainly revolved in nutrient metabolism, immune regulation, etc. Further analysis revealed that important signaling pathways were triggered after infection by S. japonicum in M. fortis but not in the mice. These results provide new insights into the general mechanisms of regulation in the non-permissive schistosome host M. fortis that exploits potential miRNA regulatory networks. Such information will help improve current understanding of schistosome development and host–parasite interactions.

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