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Planta:番木瓜miRNA研究获进展

来源:中科院版纳植物园 2013-10-16 10:18

番木瓜(Carica papaya)又称木瓜,是十字花目番木瓜科水果。番木瓜不仅可以作为水果食用,还有重要的药用价值。作为一种重要的热带植物,番木瓜的产量和质量常常受到病虫害的严重影响。其中为害最普遍及最严重的首推由病毒引起的番木瓜环斑花叶病,除此之外,还有10多种真菌病害,如炭疽病、叶斑病。为了减少损失,目前已经培育了抗特异类型病毒的转基因番木瓜品种。尽管如此,单一的转基因品种仍然无法抵御其他病害的威胁。为了更好从分子水平研究番木瓜,南开大学和美国的研究机构进行的一项大型联合研究,于2008年获得了番木瓜基因组的首个草图。这个草图拼出了90%的番木瓜基因编码序列。

miRNA是一类21个核苷酸左右的小分子,它可以直接负调控其靶基因的表达。研究表明,植物miRNA参与了生长发育,营养代谢,生物和非生物逆境等多方面的功能。结合已有的小RNA深度测序结果于基因组序列,利用生物信息学技术手段,中科院西双版纳热带植物园植物分子研究组鉴定了75个保守的miRNA和11个新的miRNA,并预测了这些miRNA对应的靶基因。这些miRNA直接调控植物生长发育和抗逆等方面的关键基因。同时,该研究还鉴定了几个番木瓜特有的miRNA调控途径,这些途径可能直接参与番木瓜的抗病功能。

该研究以Identification of miRNAs and miRNAs mediated regulatory pathways in Carica papaya为题发表在Planta上(生物谷Bioon.com)。

生物谷推荐的英文摘要

Planta                         DOI:10.1007/s00425-013-1929-6

Identification of miRNAs and miRNA-mediated regulatory pathways in Carica papaya

Gang Liang, Yang Li, Hua He, Fang Wang, Diqiu Yu

Plant microRNAs (miRNAs) post-transcriptionally regulate target gene expression to modulate growth and development and biotic and abiotic stress responses. By analyzing small RNA deep sequencing data in combination with the genome sequence, we identified 75 conserved miRNAs and 11 novel miRNAs. Their target genes were also predicted. For most conserved miRNAs, the miRNA-target pairs were conserved across plant species. In addition to these conserved miRNA-target pairs, we also identified some papaya-specific miRNA-target regulatory pathways. Both miR168 and miR530 target the Argonaute 1 gene, indicating a second autoregulatory mechanism for miRNA regulation. A non-conserved miRNA was mapped within an intron of Dicer-like 1 (DCL1), suggesting a conserved homeostatic autoregulatory mechanism for DCL1 expression. A 21-nt miRNA triggers secondary siRNA production from its target genes, nucleotide-binding site leucine-rich repeat protein genes. Certain phased-miRNAs were processed from their conserved miRNA precursors, indicating a putative miRNA evolution mechanism. In addition, we identified a Carica papaya-specific miRNA that targets an ethylene receptor gene, implying its function in the ethylene signaling pathway. This work will also advance our understanding of miRNA functions and evolution in plants.

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