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Neur. Rege. Rese.:小型猪缺血性脑卒中模型的异网栓塞法

  1. 缺血性脑卒

来源:EurekAlert!中文 2013-07-12 15:38

目前国内外文献报道的缺血性脑卒中常用模型动物为啮齿类动物,常用的建模方法为大脑中动脉闭塞法。但因啮齿类动物解剖结构与人类相去甚远。近些年来人们积极探索与优化和人脑解剖结构接近的大动物脑卒中模型的建立方法。因为猪脑体积和质量与人接近,多脑回脑皮质,灰白质比例与人非常相近,便于利用常规的脑成像技术对其进行评价,同时还可以模拟人体进行常规手术操作,是一种较理想的模型动物。

目前国内外文献报道的缺血性脑卒中常用模型动物为啮齿类动物,常用的建模方法为大脑中动脉闭塞法。但因啮齿类动物解剖结构与人类相去甚远。近些年来人们积极探索与优化和人脑解剖结构接近的大动物脑卒中模型的建立方法。因为猪脑体积和质量与人接近,多脑回脑皮质,灰白质比例与人非常相近,便于利用常规的脑成像技术对其进行评价,同时还可以模拟人体进行常规手术操作,是一种较理想的模型动物。但因为猪脑颈内动脉与咽升动脉之间有一个特征性毛细血管网(异网),这使得常规介入栓塞大脑中动脉的方法不适用于小型猪脑卒中模型的建立。

利用新型可降解材料海藻酸钠微球介入栓塞小型猪颈内动脉起始端的毛细血管网(异网)的方法成功建立了小型猪缺血性脑卒中模型,这有望突破一直阻碍小型猪成为缺血性脑卒中疾病常用模型动物的瓶颈问题。为脑缺血的病理生理机制研究,以及治疗新药开发提供一种相对经济、操作简便的新模型。(生物谷 Bioon.com)

生物谷推荐的英文摘要

Neural Regeneration Research  DOI: 10.3969/j.issn.1673-5374.2013.16.004

Application of sodium alginate microspheres in ischemic stroke modeling in miniature pigs

Cui YC, Tian Y, Tang Y, Jia LJ, Wu AL, Peng P, Yang JZ, Du H, Wang XJ, Wu LK.

The miniature pig is an optimal animal model for studying nervous system disease because of its physiologic and pathologic features. However, the rete mirabile composed of arteries and veins at the skull base limits their application as a model of ischemic stroke by middle cerebral artery occlusion. The present study investigated the possibility of establishing an ischemic stroke model in the miniature pig by blocking the skull base retia with sodium alginate microspheres. Three Bama miniature pigs were used. Using the monitor of C-arm X-ray machine, sodium alginate microspheres (100–300 μm), a novel embolic material, were injected through the femoral artery, aortic arch, common carotid artery, ascending pharyngeal artery and the retia. Results were evaluated using carotid arteriography, MRI, behavior observation and histology. The unilateral rete mirabile was completely blocked, resulting in disturbance in blood supply to the basal ganglia, astasia of the right hind limb and salivation. MRI and hematoxylin-eosin staining showed an evident infarction focus in the basal ganglia. These findings indicate that sodium alginate microspheres are a suitable embolic material for blocking the skull base retia in miniature pigs to establish an ischemic stroke models.

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