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Plant Physiol:王秀杰等揭示miRNA在油菜油份积累和胚胎发育中的作用

来源:中科院遗传与发育生物学研究所 2011-12-12 21:00

12月2日,国际著名杂志Plant Physiology刊登了中国科学院遗传与发育生物学研究所等相关机构研究人员的最新研究成果“Small RNA profiling in two Brassica napus cultivars identifies microRNAs with oil production and developmental correlated expressions and new small RNA classes 。”

越来越多的证据表明,miRNA与siRNA这些小分子RNA在植物的生长发育和种子形成等过程中起着重要的调控作用。但目前在油料作物油菜中关于小分子RNA的研究还很少,尤其关于miRNA与siRNA在油菜胚胎发育过程和种子油份积累中表达与功能的研究更少。

中国科学院遗传与发育生物学研究所王秀杰课题组与江苏农业科学院经济作物研究所戚存扣课题组合作,通过选取种植在南京与拉萨两种不同环境的高油和低油油菜品系作为实验材料,系统分析了其胚胎发育早期表达的miRNA及其表达变化情况。该研究发现了50个保守的miRNA和11新的miRNA,并且鉴定了一些miRNA的靶基因。

实验研究显示,部分miRNA在不同的油份含量品系中或胚胎发育过程中呈现出不同的表达水平,提示其可能参与调控油菜油份的积累和胚胎的早期发育。此外,该研究还发现油菜种子中表达大量长度为23 nt的small RNA,并且这些small RNA在序列组成上具有一定的核苷酸偏好性,预示着它们可能代表一种新类型的功能small RNA。

王秀杰课题组的博士研究生赵营涛和助理研究员王猛博士为该论文的共同第一作者。该项研究得到国家自然科学基金委和科技部973项目的资助。(生物谷Bioon.com)

Small RNA profiling in two Brassica napus cultivars identifies microRNAs with oil production and developmental correlated expressions and new small RNA classes

Ying-Tao Zhao1 (ytzhao@genetics.ac.cn), Meng Wang1 (mengwang@genetics.ac.cn), San-Xiong Fu2 (naufsx@163.com), Wei-Cai Yang1 (wcyang@genetics.ac.cn), Cun-Kou Qi2 (qck@jaas.ac.cn) and Xiu-Jie Wang13 (xjwang@genetics.ac.cn)

MicroRNAs and siRNAs are important regulators of plant development and seed formation, yet their population and abundance in the oil crop Brassica napus are still less understood, especially at different developmental stages and among cultivars with varied seed oil contents. Here, we systematically analyzed the small RNA expression profiles of Brassica napus seeds at early embryonic developmental stages in a high oil content and a low oil content Brassica napus cultivars, both cultured in two environments. A total of 50 conserved miRNAs and 11 new miRNAs were identified, together with some new miRNA targets. Expression analysis revealed some miRNAs with varied expression levels in different seed oil content cultivars or at different embryonic developmental stages. A large amount of 23-nt small RNAs with specific nucleotide composition preference were also identified, which may present new classes of functional small RNAs.

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