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Lab Chip:玻璃芯片中的微米级肺泡

来源:厦门大学 2009-09-19 11:50

英国皇家化学会“芯片实验室”杂志于2009年9月16日发表了厦门大学彭兴跃副教授细胞芯片研究的最新进展。

该研究成功地在玻璃材质的芯片中制作了微米级的肺泡,可用于芯片中的气体交换及气体梯度控制。长期以来,由于玻璃等非透气性材质的芯片无法实现气体交换,科学家门不得不转而采用其他透气性材料,如PDMS。但玻璃材质的光学性能优越,其他材料却不仅光学性能不如玻璃,其可能的毒性也使得细胞在芯片内的环境受到干扰。彭兴跃副教授抛弃了转而求其次的想法,巧妙利用表面张力在硬质非透气材料中形成了微米级的肺泡,不仅能够进行气体交换,还能够控制形成显微镜下可视的微米级梯度,从而攻克了这一个细胞芯片的难题。研究生吴兰亲、张娜、胡丽丹以及07级本科生李游和李文娟也参与了部分实验和数据处理工作。(生物谷Bioon.com)

生物谷推荐原始出处:

Lab Chip, 2009 DOI: 10.1039/b818738j

A vast-range speed control microchip for retention of all cell types

Xing Yue (Larry) Peng, Ling Qiao Li and Hua Sheng Hong

This Technical Note is the first description of a large-scale logarithmic flow-rate damping system designed to retain cells of different adherence, different suspensibility and different motility. The chamber, which can easily retain and cultivate many types of cells, including high-motility cells and swimming cells, via a series of speed bumps, readily facilitates cell retention for complex heterogeneous cultures. Yeast cells, red blood cells, rabbit bone marrow aspirate and dinoflagellate swimming cells were introduced into the chip for multi-cell retention, multi-cell culture and observation. Here, we show that the chamber creates a flow field with a ratio of end/start speeds as low as 0.01. The logarithmic distribution of flow-rate within the chamber is controlled precisely by pressure, all of the cell types that we tested were retained easily within the chamber. Many cell–cell interactions were observed, predicting a high potential for the success of on-chip heterogeneous cell experiments.

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