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PNAS:RNA干扰研究新进展

来源:生物谷 2007-06-14 09:06

   

    生物谷报道:在6月11日的PNAS网络版上,来自哈佛大学分子和细胞生物学系的研究人员发表的论文证实秀丽隐杆线虫(Caenorhabditis  elegans)SID-2是环境RNA干扰(RNAi)所必须的。

    在植物和线虫(秀丽隐杆线虫)中,一种RNAi信号能够引发细胞中远距离位点的基因沉默。而且,在线虫中,环境中或其吞食的细菌中表达的dsRNA足以音符RNAi,即环境RNAi  dsRNA的摄入和吞食在其他无脊椎动物中也曾有过描述。在哈佛的这项新研究中,研究人员确定并分析了SID-2(一种肠道luminal跨膜蛋白质)是线虫中发生环境RNAi所必须的。当在不发生环境RNAi的线虫种(Caenorhabditis  briggsae)中表达SID-2时,能够使这种线虫拥有了环境RNAi。

原始出处:

Published online before print June 11, 2007
Proc. Natl. Acad. Sci. USA, 10.1073/pnas.0611282104
Genetics

Caenorhabditis elegans SID-2 is required for environmental RNA interference

( dsRNA | transmembrane | intestine lumen )

William M. Winston *, Marie Sutherlin , Amanda J. Wright , Evan H. Feinberg , and Craig P. Hunter ¶

Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138

Edited by Iva S. Greenwald, Columbia University, New York, NY, and approved March 6, 2007 (received for review December 19, 2006)

Abstract

In plants and in the nematode Caenorhabditis elegans, an RNAi signal can trigger gene silencing in cells distant from the site where silencing is initiated. In plants, this signal is known to be a form of dsRNA, and the signal is most likely a form of dsRNA in C. elegans as well. Furthermore, in C. elegans, dsRNA present in the environment or expressed in ingested bacteria is sufficient to trigger RNAi (environmental RNAi). Ingestion and soaking delivery of dsRNA has also been described for other invertebrates. Here we report the identification and characterization of SID-2, an intestinal luminal transmembrane protein required for environmental RNAi in C. elegans. SID-2, when expressed in the environmental RNAi defective species Caenorhabditis briggsae, confers environmental RNAi.

 

Fig. 1. sid-2 worms are capable of spreading transgene-initiated RNAi. (A)Wild-type expression of GFP in the pharynx (ph) (myo-2::gfp) and body-wall muscle nuclei (bm) (myo-3::gfp) of an HC46 worm. (B) Expression of dsRNA (myo-2::gfp) in the pharynx causes incomplete silencing of pharynx and body-wall muscle GFP in the HC57 strain. (C)Asid-2(qt13)wormexpressing the same transgenes as HC57 is capable of spreading transgene-initiated RNAi from the pharynx to the body-wall muscle. GFP images were taken at equal exposures. Anterior is upper left. (Scale bar, 0.1 mm.)

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