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Cytotherapy:婴儿脐血干细胞培育出肺泡细胞

来源:生物通 2006-11-06 11:26

        明尼苏达大学研究人员首次将脐血干细胞(umbilical  cord  blood  stem  cells)转化为II型肺泡细胞(type  II  alveolar  cell)。研究结果刊登于11月7日《Cytotherapy》杂志。

        II  型肺泡细胞分泌的表面活性剂(surfactant)使肺部气囊(air  sacs)保持开启状态,空气能够进出气囊。II  型肺泡细胞也能帮助受损气道复原。

        “将来,我们能够通过检测患有囊肿性纤维化(cystic  fibrosis)等肺部疾病的婴儿的脐血,研究这些疾病的成因,研制更好的医疗措施。”  

        明尼苏达大学医学中心临床细胞治疗实验室医学主任、医学和病例学实验室副教授David  McKenna说。

        II  型肺泡细胞出现于胚胎发育晚期,因此一些早产婴儿的肺部发育不全。婴儿出生的头几天,II型肺泡细胞和气囊作为一个整体将继续发育、成熟。

        目前,研究人员对脐血转化的II型肺泡细胞进一步观察,希望这些细胞有朝一日能够作为一种研究工具用于研究肺部发育和肺部疾病,检测新药的途径。

        为了将肺细胞从脐血细胞中分离出来,McKenna等首先将脐血干细胞转化为多系祖细胞(Multi-Lineage  Progenitor  Cell™  ,MLPC™,生物通编者译)。BioE®,  Inc的St.  Paul首次分离和鉴定出的一种干细胞,能够在培养基中扩大培养,然后分化为构成胚胎外胚层、中胚层、内胚层的各种组织类型。

        McKenna及其同事在培养基中培养MLPC,将其转化为肺细胞(一种内胚层细胞),再利用多种方法寻找携带II型肺泡细胞关键标记的细胞,经过这一系列步骤最终得到II  型肺泡细胞。

英文原文:

Researchers Grow a Lung from Stem Cells

Recently we covered how researchers are using stem cells to grow new livers, breasts, and even faces. Now scientists at the University of Minnesota have teamed up with BioE Inc., to grow type II alveolar cells from stem cells.

The cord blood cells differentiated into a type of lung cell called type II alveolar cells. These cells are responsible for secreting surfactant, a substance which allows the air sacs in the lungs to remain open, allowing air to move in and out of the sacs. The cells are also responsible for helping to repair the airway after injury.

"In the future, we may be able to examine cord blood from babies who have lung diseases, such as cystic fibrosis, to do more research to understand how these diseases evolve as well as to develop better medical treatments," said David McKenna, M.D., assistant professor of lab medicine and pathology and medical director of the Clinical Cell Therapy Lab at the University of Minnesota Medical Center, Fairview.

The research paper is currently available online, and will be published in the Nov. 7, 2006, issue of the journal Cytotherapy.

Type II alveolar cells develop late in fetal development, which is why some premature babies are born with underdeveloped lungs. The cells and the air sacs as a whole continue to mature and develop through a child's first few years of life.

Now the researchers will try to better characterize the cells, so that in the future, the cells could be used as a research tool to better understand lung development and disease. The cells may also be useful as a way to test potential new drugs.

To differentiate the lung cells from the cord blood, McKenna and his team first derived the Multi-Lineage Progenitor CellTM (MLPCTM) from umbilical cord blood. This stem cell, which was first isolated and characterized by BioE, Inc., St. Paul, is a precursor cell that can be expanded in culture, then differentiated into different types of tissue representative of all three embryonic lineages, endoderm, mesoderm and ectoderm.

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