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杨清——南京农业大学——植物分子生物学、植物基因组学与基因工程

  1. 杨清,南京农业大学

来源:生物谷 2016-07-26 17:34

植物分子生物学、植物基因组学与基因工程

导师姓名:杨清         导师类别:博士生导师  

  • 姓名: 杨清       性别: 男       出生年月:
  • 所在院校: 南京农业大学       所在院系: 生命科学学院
  • 职称: 教授       招生专业: 植物学
  • 研究领域: 植物分子生物学、植物基因组学与基因工程
  • 联系方式

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  • 个人简介

      一、个人信息
      杨清,男,留法博士, 2000年江苏省政府海外引进人才、南京农业大学特聘教授、博士生导师, 分子生物学实验室、马铃薯研究室负责人。
      二、主要学历
      1986.1-1989.1: 法国雷恩国立农业大学(ENSAR)植物科学系,植物生物工程专业博士;
      1984.2-1985.12: 法国雷恩国立农业大学农学系,植物遗传改良专业硕士;
      1978.2-1982.2: 南京农业大学园艺系,蔬菜专业学士。
      三、工作经历
      2002年进入生命科学学院工作至今;
      2000-02年,在南京农业大学生物科技学院工作,任特聘教授、博士生导师、研究生分子生物学实验室主任,副院长;
      1998-2000年, 在加拿大蒙特利尔大学(University of Montreal)植物生物学研究所(IRBV)工作、研究科学家;
      1992-97年, 在加拿大魁北克大学蒙特利尔分校(UQAM), 博士后、研究科学家;
      1989-91年, 在法国国家农业研究院(INRA)雷恩研究中心做博士后研究。
      四、研究领域
      1.植物分子生物学
      2.植物基因组学与基因工程
      3.马玲薯分子生物学、分子改良和生物技术
      五、研究概述
      1984-2000年,在法国和加拿大留学、工作,从事植物雄性生殖、单倍体、生物技术、分子生物学等研究。
      2000年至今,在南京农业大学工作,从事植物基因组学、基因工程、马玲薯生物技术和分子改良研究。回国后,先后承担国家教育部、科技部、外专局和省科技厅课题多项。
      主要成果:提出花椰菜离体雄性生殖的模型与机理; 建立了花椰菜小孢子单倍体再生体系,首次成功获得单倍体植株; 建立了高效花药悬浮培养体系; 建立了单倍体快速检测鉴定方法; 创立了花蕾培养诱导单倍体新方法; 建立了落叶松成熟材料原生质体再生体系和再生潜力细胞筛选的数学模型; 利用不同倍体材料分析并提出马铃薯自交不亲和性的新证据,进一步完善了自交不亲和性的分子机理; 克隆了包括自交不亲和等位基因在内7个功能基因,并在GenBank上登录; 建立了马铃薯高效离体快繁体系; 建立了不同倍体不同生态型马铃薯微型薯诱导方法; 利用T-DNA突变体,研究拟兰芥抗旱性,分离了若干个与抗旱相关的基因; 研究水稻耐盐性遗传,克隆了两个与耐盐相关的新基因; 建立了农杆菌介导的马铃薯转基因体系,获得了MSI-99转基因植株; 建立烟草叶绿体基因工程体系,将中长链羟基脂肪酸聚酯合成基因导入烟草; 研究多枝赖草耐盐机理,克隆了耐盐相关基因的cDNA片段; 研究与阐明了洋葱CMS不育机理; 研究马铃薯花青苷生物合成,克隆了4个关键酶基因; 研究茄子黄萎病抗性,克隆了两个相关基因; 建立了野生马铃薯微型薯诱导技术体系; 分离到马铃薯两个光敏色素基因; 2004-05年在Genbank上登录的克隆基因:DQ020574、DQ025757、AY942199、AY942200、AY328087、 AY360145、AY781786、AY954034、AY954033、AY954034、AY954035。

    著作及论文

      近期论文
      张易,杨清,王全逸,郭建林,梁峰 (2007), 野生种马铃薯SpPHYB基因的克隆与分析.植物生理学通讯,(in press)
      沈丽珍, 梁峰, 杨清 (2007), 马铃薯内生细菌stCB1的鉴定与促生作用. 江苏农业科学,(in press)
      J. L Guo,Q. Yang,F. Liang, Y. J. Xing and Z. Wang, (2007), Molecular Cloning and Expression Analysis of a Novel CONSTANS-like From Potato. Biochemistry, (in press)
      Feng LIANG, Li-Zhen SHEN, Min CHEN and Qing YANG (2007), Formation of intercellular gas space in the diaphragm during the development of aerenchyma in the leaf petiole of Sagittaria trifolia. Aquatic Botany, (in press)
      LIANG Feng, WANG Zhong, GUO Jian-lin, SHEN Li-Zhen, YANG Qing (2007), Dynamic changes of mineral element in the cell wall of growth cells detected by CSEM-EDX*. Pregress in Biochemistry and biophysics, (in press)
      Song-He Zhang, Qing Yang and Rong-Cai Ma (2007), Erwinia carotovora ssp. carotovora Infection Induced "Defense Lignin" Accumulation and Lignin Biosynthetic Gene Expression in Chinese Cabbage (Brassica rapa L. ssp. pekinensis). Journal of Integrative Plant Biology, 49 (7): 993-1002
      Feng Qiao, Qing Yang, Chun-Lian Wang, Ying-Lun Fan, Xue-Feng Wu and Kai-Jun Zhao, (2007) Modification of plant height via RNAi suppression of OsGA20ox2 gene in rice. Euphytica, 158 (1-2): 35-45
      卢其能,杨清(2007), 马铃薯花色苷种类,含量和稳定性初步研究. 安徽农业科学, 35(16) : 4811 - 4813
      蒋敏华,杨清,李丽,黄先群,(2006),马铃薯抗晚疫病转基因研究进展, 种子, 25(12):46-50
      Song-He Zhang, Qing Yang and Rong-Cai Ma (2006), Isolation, characterization and expression analysis of BrMyb from Erwinia carotovora subsp. Carotovora diseased Chinese cabbage. African Journal of Biotechnology, 6 (5): 516-522
      Zhang Y, Guo JL, Lu QN, Yang Q* (2006), Cloning and expression analysis of PHYA cDNA from wild potato (Solanum pinnatisectum). Plant Physiology Communications, 42(6): 1081-1086
      XIAO Xue, ZHANG Xiu-hai, YANG Qing, ZOU Hua-wen, WU Zhong-yi, YU Rong, CAO Ming-qing, HUANG Cong-lin (2006), Subcellular Localization of ZmSPK1, A Protein Kinase from Maize, in Transgenic Arabidopsis. ACTA AGRICULTURAE BOREALI-SINICA, 21(3): 1-4
      Li YY, Wang Z, Liang F, Shen LZ, Yan JY, Yang Q* (2006), RAPD analysis of the genomic DNA of CMS line 70 and its maintainer line 71 in onion. Biotechnology Bulletin, 6: 100-102
      Yang Q* and Zhang Y (2006), In vitro induction of microtubers in Solanum cardiophyllum. 11th IAPTC&B congress, Beijing, China, August 13-18
      Wang Z, Yin Y, Yang Q* and Chen M (2006), Cloning and analysis of cDNA fragment of PGIP gene from Solanum torvum. 11th IAPTC&B congress, Beijing, China, August 13-18
      Lu QN,Yang Q* and Zou HW(2006), Effects of cerium on the accumulation of anthocyanins and expression of anthocyanin biosynthetic genes in in-vitro potato tissue . Journal of rare earths 24(4):479-484
      Lu QL and Yang Q* (2006), Cloning and expression of anthociaynin biosynthetic genes in wild potato. African Journal of Biotechnology 5(10):811-818
      Zou Huawen, Wu Zhongyi, Xiao Xue, Zhang Xiuhai, Cao Mingqing, Jia Wensuo, Huang Conglin* and Yang Qing* (2006), Gene expression analyses of ZmPti1, encoding a maize Pti-like kinase, suggest a role in stress signaling. Plant Science 171:99-105
      Zou Huawen, Yang Qing, Zhang Xiuhai, Zhao JiuRan, Wu Zhongyi, Wang Fengge and Huang Conglin(2006), Cloning and Characterization of Maize ZmSPK1, a Homologue to Nonfermenting1-Related Protein Kinase2. African Jounal of Biotechnology 5(6):490-496
      Shi RJ, Yin Y, Wang Z, Chen M and Yang Q* (2006), Cloning and analysis of full-length cDNA of StoVe1 gene from Solanum torvum. Plant Physiology Communication 44(4):638-642 (in Chinese)
      Xu LL, Lu D, Lu W, Zhang RX and Yang Q* (2006), Establishment of suspension cell line of rice (Oryza sativa L.) and effects of different media on biomass. Plant Physiology Communication 44(4):612-616 (in Chinese)
      Li Y Y, Yang Q* and Yan J Y and Chen M (2006), Cytoplasmic Male-Sterility of Onion (Allium cepa L.) 63A Is Associated with Premature Degeneration of the Tapetum. Acta Agronomica Sinica 32(3):369-372
      Zhang y, Yang Q* and Chen M (2006), In-vitro production of microtubers from wild potato. Acta Horticulturae Sinica 33(2):369
      Wang YH, Wu ZY, Zhang XH, Huang CL* and Yang Q* (2006), Stable transfortion of phaC2 gene in tobacco chloroplast genome and maternal inheritance of transgenes. Journal of Agricultural Biotechnology 14(2):213-218(in Chinese)
      Wang YH, Wu ZY, Zhang XH, Chen GQ, Wu Q, Huang CL* and Yang Q* (2005), Synthesis of medium-chain-length-polyhydroxyalkanoates in tobacco via chloroplast genetic engineering. Chinese Science Belletin 50 (11): 1113-1120
      Shi RJ, Zhao ML* and Yang Q* (2006), Cloning and analysis of glutathione reductase gene from Leymus multicaulis.Journal of Northwest Sci-tech University of Agriculture and Forestry 34(2):61-67(in Chinese)
      Qiao F, Fan YL, Yang Q and Zhao KJ (2006), RNAi molecular mechanism and its application in plant functional genomics research. Biotechnology belletin 4: 20-24
      Xiao X , Zhang X , Yang Q , Zou HW , Wu ZY , Yu R , Cao MQ and Huang CL (2006), Subcellular Localization of ZmSPK1 , A Protein Kinase from Maize , in Transgenic Arabidopsis. Acta Agriculturae Boreali-Sinica 21(3):1-4
      Shi RJ, Zhao ML* and Yang Q* (2005), Cloning and Analysis of cDNA segment of glutathione reductase in Leymus multicaulis. Acta Bot. Boreal.-Occcident. Sin. 25(5): 881-886 (in Chinese)
      Zhao CL, Guo WM, Yang Q and Chen JY (2005), Cloning of full-length F3’H of Nanjing hongxu (Prunus Mume) by gDNA-based TAIL-PCR. Acta Bot. Boreal.-Occcident. Sin.25(12): 2378-2385
      HAO G P, WU Z Y, CAO M Q, Pelletier G, HUANG C L*, and YANG Q* (2004),Nucleotide polymorphism in the drought induced transcription factor CBF4 locus region of Arabidopsis thaliana and its molecular evolution analysis. Acta Genetica Sinica 31(12): 1415-1425 (in Chinese)
      HAO G P, WU Z Y, CHEN M S, CAO M Q, Pelletier G, HUANG C L*, and YANG Q *(2004), ATHK1 gene regulates signal transduction of osmotic stress in Arabidopsis thaliana. Journal of plant physiology and molecular biology 30(5): 553-560 (in Chinese)
      ZHAO W F , YANG Q* and CHEN M (2004), Host cell factors influencing the transfer of T-DNA to plant. Plant Physiology Communication 40(4): 521-526 (in Chinese)
      WANG Y H, YANG Q* and CHEN M (2004), Suger sensing and signaling in plants. Chinese Bulletin of Botany 21(3): 273-279 (in Chinese)
      HAO G P, WU Z Y, CHEN M S, CAO M Q, BRUNNEL D, Pelletier G, HUANG C L*, and YANG Q* (2004) Single nucleotide polymorphisms of CBF4 locus region of Arabidopsis thaliana correspond to drought tolerance. Journal of Agricultural Biotechnology 12(2): 122-131 (in Chinese)
      HAO G P, YANG Q, WU Z Y, CAO M Q and HUANG C L (2004) Single nuleotide polymorphism (SNP) and its applications in crop genetics and breeding. Chinese Bulletin of Botany 21(5): 618-624 (in Chinese)
      HAO G P,DU X H,SHI R J,ZHANG S H,ZHANG H J and YANG Q*,(2003) Calli induction from in vitro culture of tender stem segments in Ginkgo biloba and study on its cytology. Acta Bot. Boreal.-Occcident. Sin. 23(4):648-652 (in Chinese)
      HAO G P,CHEN M and YANG Q*,(2003) Progress on study of plant mitochondria and cytoplasmic male sterility. Chinese Bulletin of Botany 20(5):549-557 (in Chinese)
      HAO G P and YANG Q*,(2002) Progress in molecular mechanism of cytoplasmic male sterility and fertility restoration in plants. Subtropical Plant Science 31 (3): 78-84 (in Chinese)
      HAO G P and YANG Q*,(2002) Advances in intein. Life Science Research 6(4): 136-139 (in Chinese)
      QIN X, LUU D T, YANG Q, MAES O, MATTON D, MORSE D and CAPPADOCIA M, (2001) Differences in S12-RNase expression in Solanum chacoense correlate with pseudo-self-incompatibility. Plant Molecular Biology 45: 295-305
      LUU D T, QIN X, LAUBLIN G, YANG G, MORSE D, CAPPADOCIA M, (2001) Rejection of diploid S-heteoallelic pollen by a dual specific S-RNase in Solanum chacoense. Genetics 159 (1): 329-335

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